Line 38: | Line 38: | ||
<br> | <br> | ||
<h1>Current from Induction</h1> | <h1>Current from Induction</h1> | ||
+ | |||
+ | <div class="w3-cell-row w3-pale-green" style="width:100%"> | ||
+ | <div class="w3-container w3-cell"> | ||
+ | <div class="w3-card-4 w3-pale-blue" style="width:100%"> | ||
+ | <div class="w3-container w3-center"> | ||
+ | <h3>Current correlating to YouTube video</h3> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/e/e2/MSU-Michigan_current.png" style="width:100%;max-width:600px"> | ||
+ | <p>Current graph correlating to the YouTube video above. This current is also displayed under Measurement to compare current as a reporter for protein expression to GFP fluorescence under anaerobic conditions.</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
<div class="w3-cell-row w3-pale-green" style="width:100%"> | <div class="w3-cell-row w3-pale-green" style="width:100%"> |
Revision as of 21:52, 1 November 2017
Demonstrate
MSU iGEM has succeeded in inducing current from induction of a selected chemical.
We measured the results using both IPTG and current.
Below is a video showing the working bioreactors outputting current.
Current from Induction
Current correlating to YouTube video
![](https://static.igem.org/mediawiki/2017/e/e2/MSU-Michigan_current.png)
Current graph correlating to the YouTube video above. This current is also displayed under Measurement to compare current as a reporter for protein expression to GFP fluorescence under anaerobic conditions.
deltamtrB_GFPmtrB Induced V Uninduced
![](https://static.igem.org/mediawiki/2017/b/be/T--MSU-Michigan--currentmtrB170725.png)
Same full vector strain but induction at point shown displaying the actuality of IPTG inducing current increase. This shows the difference between background expression and actual induction very distinctly.
deltamtrB_GFPmtrB Induced V Uninduced
![](https://static.igem.org/mediawiki/2017/b/be/T--MSU-Michigan--currentdeltamrtBGFPinduced.png)
The earlier graph compared to the one above. Shows how viability of cells earlier on produces a larger current increase compared to background expression.
Multiple Strains
![](https://static.igem.org/mediawiki/2017/d/d7/T--MSU-Michigan--current170714.png)
Induction occurring at around 22 hours showing a doubling in current. The background control strains are shown here which display how little current is produced when mtrB is not present.
Sponsors | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
![]() |
![]() |
![]() |
![]() |
![]() |
![]() |
![]() |
![]() |
![]() |
![]() |
Contact us:
![]() ![]() ![]() |