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− | <h4>1.pSBC3- | + | <h4>1.pSBC3-pTDH3+ceas2+tPFK1(BBa_K2347005)<br><h4> |
− | <h4>In this part, the promoter of | + | <h4>In this part, the promoter of ceaS2 is pTDH3 promoter, and the terminator is tPFK1 terminator. pTDH3 promoter is a kind of promoter which has strong expression and tPFK1 terminator is a terminator with rather strong expression ability. So they can both increase the expression of genes. At the same time they are constitutive promoters and terminators, which have the ability to integrate gene fragment, ceaS2, into the chromosome of yeast, thereby reducing the burden of plasmid expression in yeast. We linked this part with the YCplac33 plasmid vector to form intact plasmid with LEU deficient. |
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</h4><br> | </h4><br> | ||
− | <h4>2.pSBC3- | + | <h4>2.pSBC3-ADH1+gld+tGPD1(BBa_K2347006)<br><h4> |
− | <h4>In this part, the promoter of | + | <h4>In this part, the promoter of gld is ADH1 promoter, and the terminator is tGPD1 terminator. ADH1 promoter is a kind of promoter which has strong expression and tGPD1 terminator is a terminator with rather strong expression ability. So they can both increase the expression of genes. At the same time they are constitutive promoters and terminators, which have the ability to integrate gene fragment, gld, into the chromosome of yeast, thereby reducing the burden of plasmid expression in yeast. We linked this part with part pSBC3-PGK1 + DAK + TPFK1 and the YCplac33 plasmid vector to form intact plasmid with URA deficient. |
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</h4><br> | </h4><br> | ||
− | <h4>3.pSBC3- | + | <h4>3.pSBC3-PGK1+DAK+tPFK1(BBa_K2347007)<br><h4> |
<h4>In this part, the promoter of ceaS2 is pTDH3 promoter, and the terminator is tPFK1 terminator. pTDH3 | <h4>In this part, the promoter of ceaS2 is pTDH3 promoter, and the terminator is tPFK1 terminator. pTDH3 | ||
promoter is a kind of promoter which has strong expression and tPFK1 terminator is a terminator with | promoter is a kind of promoter which has strong expression and tPFK1 terminator is a terminator with | ||
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with the YCplac33 plasmid vector to form intact plasmid with LEU deficient. | with the YCplac33 plasmid vector to form intact plasmid with LEU deficient. | ||
</h4><br> | </h4><br> | ||
− | <h4>4.pSBC3-TEF2+NOX+tRPS2<br><h4> | + | <h4>4.pSBC3-TEF2+NOX+tRPS2(BBa_K2347008)<br><h4> |
− | <h4>In this part, the promoter of NOX is TEF2 promoter, and the terminator is tRPS2 terminator. TEF2 promoter is | + | <h4>In this part, the promoter of NOX is TEF2 promoter, and the terminator is tRPS2 terminator. TEF2 promoter is a kind of promoter which has strong expression and tRPS2 terminator is a terminator with rather strong expression ability. So they can both increase the expression of genes. At the same time they are constitutive promoters and terminators, which have the ability to integrate gene fragment, NOX, into the chromosome of yeast, thereby reducing the burden of plasmid expression in yeast. We linked this part with part pSBC3-pTDH3+ceas2+tPFK1and the YCplac33 plasmid vector to form intact plasmid with LEU deficient. |
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</h4><br> | </h4><br> | ||
Revision as of 22:02, 1 November 2017