Difference between revisions of "Team:UCopenhagen/Attributions"

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                         <h1> A T T R I B U T I O N S</h1>
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                     <h2 class="section-heading">Introduction </h2>
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                     <p class="lead">Our team believes that establishing a stable platform for scientists to create naïve orthogonal living compartments, would allow for an unpredictable advancement in the field of synthetic biology. Our project will not attempt to create an endosymbiont, but instead investigate the mechanisms in free-living cells in a bottom-up approach to endosymbiosis.
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The endosymbiotic theory, formulated in the early years of the previous century, outlines that the organelles of the eukaryotic cell, such as the mitochondria, have their origin in free-living prokaryotes engulfed by bigger cells. These incorporated cells then co-evolved with their host conferring to it novel emergent properties which ultimately helped fuel the development of more complex multicellular biological systems such as plants and animals (Archibald, 2015). </p>
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The team worked together to come up with the idea for the project, design and perform the laboratory experiments, write the funding applications, plan and do our human practice activities and code and write the wiki. <br><br>
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But we couldn’t have done it alone. The following lists all the people who has helped us, and how they have supported us.
 +
<br><br>
 +
<br>
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<strong>General support</strong>
 +
Professor Sotirios Kampranis is our PI and helped us during the vital brainstorming process. He aided throughout the project with his expertise from the UCPH-course “Synthetic Biology”, which he teaches.
 +
<br><br>
 +
PhD Konstantinos Vanvitas was also deeply involved in converting our ideas into a project. Centre coordinator Nanna Heinz and PhD student Cecilie Hansen, who both previously have been involved in iGEM, also advised us throughout the project.
 +
<br><br>
 +
The team members of UCopenhagen team 2016 Cosmocrops advised us on the crucial initial steps of shaping a successful iGEM project.
 +
<br><br>
 
<br>
 
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<p>We have identified three mechanisms we believe to be mandatory for the development of a stable endosymbiotic relationship, which we will be trying to replicate in free-living cells. First of all, in order for the relationship to be stable, the two organisms must  be mutually dependent on each other; there must be a mutually beneficial interaction between host and symbiont. Secondly, there has to be some sort of control and synchronization of symbiont replication. If the symbiont were to be replicating freely we could end up with way too many or not enough symbionts in the host.  Finally, a common feature of the endosymbiotic organelles we have looked at, is the transfer of genes from the symbiont to the host. Because of this transfer, the gene and protein expression is taking place in the nucleus and the proteins and metabolites are transported to the organelle. This import of proteins is interesting not just for understanding endosymbiosis, but also for the potential applications in synthetic biology.</p>
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<strong>Fundraising support<strong>
 +
Centre coordinator Nanna Heinz advised our funding team on how to approach companies professionally both verbally and in writing.
 +
<br><br><br>
  
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<br>
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<strong>Laboratory support</strong>
 +
Immensely valuable support was provided by Postdoc Simon Dusséaux and Anastasia Anthanasakoglou who aided in protocol design as well as supervised our laboratory techniques.
 +
<br><br>
 +
<ul style="text-align:left; color:white;">
 +
<li><strong>Yeast work</strong><br>
 +
Simon provided us a modification (AM94) of the yeast strain S288C.</li>
 +
<li><strong>Cells and vectors</strong><br>Anastasia and Simon provided us several <i>E.coli</i> strains; mach1 for cloning,
 +
BL21 competent cells for expression and MG1655 for amplification of genes from
 +
gDNA. Anastasia also kindly provided the pRSET vector, the pET102 vector as well
 +
as His antibodies.</li>
 +
<li><strong>Protocol design</strong><br>
 +
Simon, Anastasia and Sotirios aided us thoroughly in protocol design and
 +
experimental procedures.
 +
</li>
 +
</ul>
 +
</p>
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<p class="lead">
 +
<br><br>
 +
Laboratory technician Lene Dansten runs the mandatory safety tours of the laboratories and also gives practical support to everyone at PLEN.
 +
<br><br><br>
 +
<strong>Difficult technique support</strong><br>
 +
Associate Professor Henning Jørgensen in the Plant and Soil Science section at the Department of Plant and Environmental Sciences kindly allowed us access their HPLC-MS facilities.<br><br>
 +
PhD student Rasmus Dahl-Lassen and laboratory coordinator Anja Hect Ivø assisted in our HPLC-MS experiments and amino acid quantification analysis.<br><br>
 +
Postdoc Simon Dusséaux aided us in the use of the departments fluorescence microscope.<br><br><br>
  
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<p>Based on these considerations, we decided to work on three distinct, but intertwined, projects pertaining to endosymbiosis, namely Interdependence, Number Control, and Protein import. We believe that by combining these three projects, a key step towards the understanding of endosymbiosis and its employment in synthetic biology will be obtained. </p>
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<strong>Wiki support</strong><br>
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                </div>
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MSc in biology Oluf Sonne Georg with a talent in drawing and art design made most of our illustrations and all of our animations. He also gave us the tools and building blocks to create our poster. <br><br><br>
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<strong>Presentation coaching support</strong><br>
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Annette Bjerre Ryhede from ARTIKULATION provided our presentation team her vital expertise on coaching and the art of presenting in front of a crowd.<br><br><br>
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<strong>Human Practices support</strong><br>
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Nanna Heinz helped us gather our thoughts and plans for human practices and outreach.<br><br>
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<ul style="text-align:left; color:white;">
 +
<li><strong>NiC</strong>
 +
Journalist Torill Kornfeldt gave a presentation on communicating with media
 and how to sell an iGEM project, which proved to be of great value in our
 interaction with PharmaDanmark
. A former iGEM’er from UCopenhagen, Will Wright, now involved in biotech business development, hosted a workshop on how to pitch business ideas. 
 +
Cand.phil Sune Holm gave a presentation on ethics in SynBio and provided important information on how to communicate science to the broad public.


 +
PhD Konstantinos Vanvitas, Assoc. professor Chris Workman, PostDoc
 James Behrendorff and Kenneth Jensen of Novozymes gave valuable
 feedback to all teams during the judging of NiC.</li>
 +
 
 +
<li><strong>Vartovs Videnskab</strong>
 +
The founders of Vartovs Videnskab Jamila Lilja and Anna Øhlenschlæger
 kindly helped us establish a venue for our public presentation of our project, synthetic biology and helped us facilitate a public debate on synthetic biology.<br>
 +
As a part of our panel discussion Cand.phil Sune Holm and Postdoc Thomas Laursen participated were featured and they gave invaluable insights into ethical applications and democratic uses of synthetic biology. They also provided an introduction to the field of synthetic biology and its applications to our listeners.<li>
 +
 
 +
<li><strong>Synapse - Life Science Connect</strong>
 +
The student driven life sciences organisation Synapse kindly featured us in their event “Synapse Lecture Session: synthetic biology”. <li>
 +
</ul>
 +
</p>
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<br><br><br>
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<p class="lead">
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Thanks to everyone involved in making Incell a successful project for us!</p>
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                    <h2 class="section-heading">Applications and Implications</h2>
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                    <<p>By understanding the basic principles behind the creation of stable endosymbiotic events we hope that in the future it will be possible to use artificial endosymbiosis as a new technology in synthetic biology, and we believe that value can be created in the foundational track of the iGEM competition. History has shown that great scientific advances has followed the implementation of new revolutionary technologies (Gershon 2003). </p>
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<p>We envision that artificial endosymbiosis could be applied in a broad range of fields, including agriculture, medicine and production of valuable compounds. A deeper understanding of the relationships intertwining endosymbionts and their hosts could unravel new knowledge applicable for the treatment of mitochondrial diseases, while a living compartment able to fixate nitrogen from the air could decrease the fertilizer use in agricultural production. </p>
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<p>However, the applications are only limited by the imagination of future users. Indeed, the game-changing role of endosymbiosis has not gone unseen to the eyes of the modern bioengineers, who predict that the establishment of a novel interaction has the potential to radically alter the host cell physiology without directly affecting the host genome (Scientific America Vol 105 pp. 36-45).</p>
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<p>Before the potential application of artificial endosymbiosis, there are many things to consider. While the current regulations regarding GMO limits what is possible to apply in agriculture and medicine, regulations regarding synthetically modified organisms (SMOs) have not yet been systematically put into place. How will a new field of SMO be regulated, and how will it influence possible applications of artificial endosymbiosis?</p>
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<p>In addition to our scientific investigation we are enthused to trigger debate about synthetic biology. We intend to podcast intriguing conversations with experts, thereby hoping to reach the general public and impel the discussion about the ethics and future prospects in combining biology and engineering.</p>
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Revision as of 22:30, 1 November 2017

A T T R I B U T I O N S

The team worked together to come up with the idea for the project, design and perform the laboratory experiments, write the funding applications, plan and do our human practice activities and code and write the wiki.

But we couldn’t have done it alone. The following lists all the people who has helped us, and how they have supported us.


General support Professor Sotirios Kampranis is our PI and helped us during the vital brainstorming process. He aided throughout the project with his expertise from the UCPH-course “Synthetic Biology”, which he teaches.

PhD Konstantinos Vanvitas was also deeply involved in converting our ideas into a project. Centre coordinator Nanna Heinz and PhD student Cecilie Hansen, who both previously have been involved in iGEM, also advised us throughout the project.

The team members of UCopenhagen team 2016 Cosmocrops advised us on the crucial initial steps of shaping a successful iGEM project.


Fundraising support Centre coordinator Nanna Heinz advised our funding team on how to approach companies professionally both verbally and in writing.


Laboratory support Immensely valuable support was provided by Postdoc Simon Dusséaux and Anastasia Anthanasakoglou who aided in protocol design as well as supervised our laboratory techniques.

  • Yeast work
    Simon provided us a modification (AM94) of the yeast strain S288C.
  • Cells and vectors
    Anastasia and Simon provided us several E.coli strains; mach1 for cloning, BL21 competent cells for expression and MG1655 for amplification of genes from gDNA. Anastasia also kindly provided the pRSET vector, the pET102 vector as well as His antibodies.
  • Protocol design
    Simon, Anastasia and Sotirios aided us thoroughly in protocol design and experimental procedures.



Laboratory technician Lene Dansten runs the mandatory safety tours of the laboratories and also gives practical support to everyone at PLEN.


Difficult technique support
Associate Professor Henning Jørgensen in the Plant and Soil Science section at the Department of Plant and Environmental Sciences kindly allowed us access their HPLC-MS facilities.

PhD student Rasmus Dahl-Lassen and laboratory coordinator Anja Hect Ivø assisted in our HPLC-MS experiments and amino acid quantification analysis.

Postdoc Simon Dusséaux aided us in the use of the departments fluorescence microscope.


Wiki support
MSc in biology Oluf Sonne Georg with a talent in drawing and art design made most of our illustrations and all of our animations. He also gave us the tools and building blocks to create our poster.


Presentation coaching support
Annette Bjerre Ryhede from ARTIKULATION provided our presentation team her vital expertise on coaching and the art of presenting in front of a crowd.


Human Practices support
Nanna Heinz helped us gather our thoughts and plans for human practices and outreach.

  • NiC Journalist Torill Kornfeldt gave a presentation on communicating with media
 and how to sell an iGEM project, which proved to be of great value in our
 interaction with PharmaDanmark
. A former iGEM’er from UCopenhagen, Will Wright, now involved in biotech business development, hosted a workshop on how to pitch business ideas. Cand.phil Sune Holm gave a presentation on ethics in SynBio and provided important information on how to communicate science to the broad public.

 PhD Konstantinos Vanvitas, Assoc. professor Chris Workman, PostDoc
 James Behrendorff and Kenneth Jensen of Novozymes gave valuable
 feedback to all teams during the judging of NiC.
  • Vartovs Videnskab The founders of Vartovs Videnskab Jamila Lilja and Anna Øhlenschlæger
 kindly helped us establish a venue for our public presentation of our project, synthetic biology and helped us facilitate a public debate on synthetic biology.
    As a part of our panel discussion Cand.phil Sune Holm and Postdoc Thomas Laursen participated were featured and they gave invaluable insights into ethical applications and democratic uses of synthetic biology. They also provided an introduction to the field of synthetic biology and its applications to our listeners.
  • Synapse - Life Science Connect The student driven life sciences organisation Synapse kindly featured us in their event “Synapse Lecture Session: synthetic biology”.




Thanks to everyone involved in making Incell a successful project for us!

Find Incell here: