Demonstrate

MSU iGEM has succeeded in inducing current from induction of a selected chemical.

We measured the results using both IPTG and current.

Below is a video showing the working bioreactors outputting current.

Current from Induction

Current correlating to YouTube video

Current graph correlating to the YouTube video above. This current is also displayed under Measurement to compare current as a reporter for protein expression to GFP fluorescence under anaerobic conditions.

deltamtrB_GFPmtrB Induced V Uninduced

Same full vector strain but induction at point shown displaying the actuality of IPTG inducing current increase. This shows the difference between background expression and actual induction very distinctly.

deltamtrB_GFPmtrB Induced V Uninduced

The earlier graph compared to the one above. Shows how viability of cells earlier on produces a larger current increase compared to background expression.

Multiple Strains

Induction occurring at around 22 hours showing a doubling in current. The background control strains are shown here which display how little current is produced when mtrB is not present.

Contaminant Induction

Nitrate GFP Induction

Induction displayed very clearly by optimal concentration levels of nitrate. It appears that contaminant levels > .5µM prove to be toxic to the cells. IPTG was added at the beginning at 100µM concentration.

Paraquat GFP Induction

Induction displayed very clearly by optimal concentration levels of hydrogen peroxide. Hydrogen peroxide was used as an inducer instead of Paraquat for safety so the promoter appears to be sensitive to multiple reactive oxygen species. It appears that contaminant levels > .5µM prove to be toxic to the cells. IPTG was added at the beginning at 100µM concentration.

Copper GFP Induction

Induction displayed very clearly by optimal concentration levels of copper. With increasing concentration of copper added up to the toxic level determined to be .1µM, GFP fluorescence increases with increasing inducer concentration. IPTG was added at the beginning at 100µM concentration.

Molybdenum GFP Induction

Induction displayed very clearly by optimal concentration levels of molybdenum. It appears that induction might have occurred with molybdenum but it is unclear (See discussion). IPTG was added at the beginning at 100µM concentration

Discussion

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