Difference between revisions of "Team:TP-CC San Diego/Model"

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This year’s interlab study is intended to answer the main question of the relativity of fluorescence when measured at different parts of the world. To make this data more reliable, iGem has asked to test some RBS devices to make gene expression reliable and precise. For this year’s interlab study, TP-CC San Diego has decided to do the plate reader protocol along with transforming the plasmids from Kit 7. <br><br><br>
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      In our first three test groups, we will only be using one guide RNA for each cell culture. The CRISPR/Cas9 will create a double strand break at the target sequence, leaving the ecDNA in two pieces.  breaks in the DNA.
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In our 4th test group, we will be using 3 guide RNAs for each cell culture. This will create double strand breaks at 3 target sequences, leaving the ecDNA in 6 pieces. We would like to test this because the more breaks we create in the ecDNA, the harder it is for the ecDNA to ligate back together.
 
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Revision as of 00:25, 2 November 2017

Modeling

The CRISPR/Cas9 system can be used for gene editing purposed including inserting genes, deleting genes, and creating breaks in the DNA.

In our first three test groups, we will only be using one guide RNA for each cell culture. The CRISPR/Cas9 will create a double strand break at the target sequence, leaving the ecDNA in two pieces. breaks in the DNA.

In our 4th test group, we will be using 3 guide RNAs for each cell culture. This will create double strand breaks at 3 target sequences, leaving the ecDNA in 6 pieces. We would like to test this because the more breaks we create in the ecDNA, the harder it is for the ecDNA to ligate back together.