Difference between revisions of "Team:Arizona State/Results"

Line 11: Line 11:
  
 
<br>
 
<br>
<p>There are 3 types of experiments used to characterize this improved receiver: F2620 Induced by Synthetic AHLs, F2620 Induced by Sender Supernatants, and F2620 induced by Sender cells on Agar. <p>
+
<p>There are 3 types of experiments used to characterize this improved receiver: Sender Quest: Battle of the AHLs, Synthetic AHLs: Quest for GFP Domination, Diffusion Quest, and Side Quest: Lost in Translation for the messing mCherry. <p>
<p>The first experiment, F2620 Induced by Synthetic AHLs, included F2620 receiver cells being induced by synthetic AHL chemicals under different concentrations. The maximum GFP expression was analyzed to understand the effect AHL concentration had on the receiver expressing GFP. <p>
+
<p>The first experiment, Sender Quest: Battle of the AHLs, used F2620 receiver cells being induced by a variety of combinations of senders in supernatant form. The maximum GFP expression was analyzed to understand the effect different percentages of sender supernatant had on the receiver expressing GFP.
 +
 
 +
included F2620 receiver cells being induced by synthetic AHL chemicals under different concentrations. The maximum GFP expression was analyzed to understand the effect AHL concentration had on the receiver expressing GFP. <p>
 
<p>The following experiment, F2620 Induced by Sender Supernatants, used F2620 receiver cells being induced by a variety of combinations of senders in supernatant form. The maximum GFP expression was analyzed to understand the effect different percentages of sender supernatant had on the receiver expressing GFP. <p>
 
<p>The following experiment, F2620 Induced by Sender Supernatants, used F2620 receiver cells being induced by a variety of combinations of senders in supernatant form. The maximum GFP expression was analyzed to understand the effect different percentages of sender supernatant had on the receiver expressing GFP. <p>
 
<p>The final experiment included F2620 receiver cells that were spread on agar plates and induced by spread sender cells. Images are then taken at different time intervals of the agar plate to analyze the induction rates and diffusion of F2620 induction. <p>
 
<p>The final experiment included F2620 receiver cells that were spread on agar plates and induced by spread sender cells. Images are then taken at different time intervals of the agar plate to analyze the induction rates and diffusion of F2620 induction. <p>
  
  
<h2>Sender Quest, Battle of the AHLs</h2>
+
<h2>Sender Quest: Battle of the AHLs</h2>
 
<p>Some of the specific questions this research was aiming to answer were: How do combinations of senders affect gene output? Are there any combinations of senders that increase the overall GFP expression? Are there any combinations that do not affect the GFP expression? </p>
 
<p>Some of the specific questions this research was aiming to answer were: How do combinations of senders affect gene output? Are there any combinations of senders that increase the overall GFP expression? Are there any combinations that do not affect the GFP expression? </p>
  

Revision as of 01:48, 2 November 2017