Difference between revisions of "Team:TNCR Korea/Part Collection"
SaintVansan (Talk | contribs) |
SaintVansan (Talk | contribs) |
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| Line 304: | Line 304: | ||
<div class="col-lg-4 col-md-4"> | <div class="col-lg-4 col-md-4"> | ||
<div class="fh5co-blog animate-box"> | <div class="fh5co-blog animate-box"> | ||
| − | <a href=" | + | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2496000"><img class="img-responsive" src="https://i.imgur.com/zfZIZJS.png" alt=""></a> |
<div class="image-popup" href="#"> | <div class="image-popup" href="#"> | ||
<div class="prod-title"> | <div class="prod-title"> | ||
| − | <h3><a href=""#>BBa_K2496000</a></h3> | + | <h3><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2496000"#>BBa_K2496000</a></h3> |
<p> = FLAG</p> | <p> = FLAG</p> | ||
</div> | </div> | ||
| Line 315: | Line 315: | ||
<div class="col-lg-4 col-md-4"> | <div class="col-lg-4 col-md-4"> | ||
<div class="fh5co-blog animate-box"> | <div class="fh5co-blog animate-box"> | ||
| − | <a href=" | + | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2496001"><img class="img-responsive" src="https://i.imgur.com/HmeqmpR.png" alt=""></a> |
<div class="image-popup" href="#"> | <div class="image-popup" href="#"> | ||
<div class="prod-title"> | <div class="prod-title"> | ||
| − | <h3><a href=""#>BBa_K2496001</a></h3> | + | <h3><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2496001"#>BBa_K2496001</a></h3> |
<p>= DPP4</p> | <p>= DPP4</p> | ||
</div> | </div> | ||
| Line 326: | Line 326: | ||
<div class="col-lg-4 col-md-4"> | <div class="col-lg-4 col-md-4"> | ||
<div class="fh5co-blog animate-box"> | <div class="fh5co-blog animate-box"> | ||
| − | <a href=" | + | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2496002"><img class="img-responsive" src="https://i.imgur.com/XcIJE1C.png" alt=""></a> |
<div class="image-popup" href="#"> | <div class="image-popup" href="#"> | ||
<div class="prod-title"> | <div class="prod-title"> | ||
| − | <h3><a href=""#>BBa_K2496002</a></h3> | + | <h3><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2496002"#>BBa_K2496002</a></h3> |
<p>= strong recombinant</p> | <p>= strong recombinant</p> | ||
</div> | </div> | ||
| Line 337: | Line 337: | ||
<div class="col-lg-4 col-md-4"> | <div class="col-lg-4 col-md-4"> | ||
<div class="fh5co-blog animate-box"> | <div class="fh5co-blog animate-box"> | ||
| − | <a href=" | + | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2496003"><img class="img-responsive" src="https://i.imgur.com/jLsYpEH.png" alt=""></a> |
<div class="image-popup" href="#"> | <div class="image-popup" href="#"> | ||
<div class="prod-title"> | <div class="prod-title"> | ||
| − | <h3><a href=""#>BBa_K2496003</a></h3> | + | <h3><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2496003"#>BBa_K2496003</a></h3> |
<p>= strong anderson promoter(BBa_J23110)</p> | <p>= strong anderson promoter(BBa_J23110)</p> | ||
</div> | </div> | ||
| Line 348: | Line 348: | ||
<div class="col-lg-4 col-md-4"> | <div class="col-lg-4 col-md-4"> | ||
<div class="fh5co-blog animate-box"> | <div class="fh5co-blog animate-box"> | ||
| − | <a href=" | + | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2496004"><img class="img-responsive" src="https://i.imgur.com/kBG2Spz.png" alt=""></a> |
<div class="image-popup" href="#"> | <div class="image-popup" href="#"> | ||
<div class="prod-title"> | <div class="prod-title"> | ||
| − | <h3><a href=""#>BBa_K2496004</a></h3> | + | <h3><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2496004"#>BBa_K2496004</a></h3> |
<p>= moderate anderson promoter(BBa_J23111)</p> | <p>= moderate anderson promoter(BBa_J23111)</p> | ||
</div> | </div> | ||
| Line 359: | Line 359: | ||
<div class="col-lg-4 col-md-4"> | <div class="col-lg-4 col-md-4"> | ||
<div class="fh5co-blog animate-box"> | <div class="fh5co-blog animate-box"> | ||
| − | <a href=" | + | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2496005"><img class="img-responsive" src="https://i.imgur.com/9XjWM1c.png" alt=""></a> |
<div class="image-popup" href="#"> | <div class="image-popup" href="#"> | ||
<div class="prod-title"> | <div class="prod-title"> | ||
| − | <h3><a href=""#>BBa_K2496005</a></h3> | + | <h3><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2496005"#>BBa_K2496005</a></h3> |
<p>= weak anderson promoter(BBa_J23116)</p> | <p>= weak anderson promoter(BBa_J23116)</p> | ||
</div> | </div> | ||
| Line 370: | Line 370: | ||
<div class="col-lg-4 col-md-4"> | <div class="col-lg-4 col-md-4"> | ||
<div class="fh5co-blog animate-box"> | <div class="fh5co-blog animate-box"> | ||
| − | <a href=" | + | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2496006"><img class="img-responsive" src="https://i.imgur.com/V6fpQTT.png" alt=""></a> |
<div class="image-popup" href="#"> | <div class="image-popup" href="#"> | ||
<div class="prod-title"> | <div class="prod-title"> | ||
| − | <h3><a href=""#>BBa_K2496006</a></h3> | + | <h3><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2496006"#>BBa_K2496006</a></h3> |
<p>= moderate recombinant</p> | <p>= moderate recombinant</p> | ||
</div> | </div> | ||
| Line 381: | Line 381: | ||
<div class="col-lg-4 col-md-4"> | <div class="col-lg-4 col-md-4"> | ||
<div class="fh5co-blog animate-box"> | <div class="fh5co-blog animate-box"> | ||
| − | <a href=" | + | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2496007"><img class="img-responsive" src="https://i.imgur.com/0POyQ57.jpg" alt=""></a> |
<div class="image-popup" href="#"> | <div class="image-popup" href="#"> | ||
<div class="prod-title"> | <div class="prod-title"> | ||
| − | <h3><a href=""#>BBa_K2496007</a></h3> | + | <h3><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2496007"#>BBa_K2496007</a></h3> |
<p>= weak recombinant</p> | <p>= weak recombinant</p> | ||
</div> | </div> | ||
Latest revision as of 02:38, 2 November 2017
Our Parts
---Part Collection---Our Part Collection
BBa_K2496000
= FLAG
BBa_K2496001
= DPP4
BBa_K2496002
= strong recombinant
BBa_K2496003
= strong anderson promoter(BBa_J23110)
BBa_K2496004
= moderate anderson promoter(BBa_J23111)
BBa_K2496005
= weak anderson promoter(BBa_J23116)
BBa_K2496006
= moderate recombinant
BBa_K2496007
= weak recombinant
Comment
Part Collection
To achieve our goal, TNCR_Korea wanted to incorporate DPP4 gene. However, DPP4 may trigger the inflammation response in case. Therefore, we needed to control its expression. TNCR_Korea team adapted the previous BioBrick Parts, named Anderson promoter (BBa_J23110, BBa_J23111, BBa_J23116), then designed three composite plasmids (strong BBa_K2496002, moderate BBa_K2496006, weak BBa_K2496007). Basically, DPP4 was inserted with Flag molecular tag for easy detection of its incorporation by checking tag’s expression (DPP4 BBa_K2496001 and Flag BBa_K2496000). There are two Pst1 sites in the DPP4 sequence, so it is not possible to access the desired 3A assembly method from iGEM. So we used infusion ligation method instead. Consequently, TNCR_Korea constituted recombinant plasmid which has Anderson promoter, DPP4, and Flag. The Range of Part Numbers are, 1) Best New Basic part: BBa_K2496000 (Flag) BBa_K2496001 (DPP4), 2) Best New composite part: BBa_K2496002 (strong), BBa_K2496006 (moderate), BBa_K2496007 (weak), 3) Best part collection ranged from BBa_K2496000 to BBa_K2496007.
