Difference between revisions of "Team:UCopenhagen/Improve"

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{{UCopenhagen-header/CSS}}
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{{template:Copenhagen-Header}}
  
 
<html>
 
<html>
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<style>
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  .myintro {
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<body>
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      display: table;
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      width: 100%;
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<!-- Header -->
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      height: 100%;
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    <a name="landsholdet"></a>
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      padding: 100px 0;
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    <div class="intro-header4">
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      text-align: center;
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        <div class="container">
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      color: #E8DBD7;
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      background: url("https://static.igem.org/mediawiki/2017/7/71/UCopenhagen_BLUE_bg.jpeg") no-repeat bottom center scroll;
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            <div class="row">
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      background-color: #264653;
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                <div class="col-lg-12">
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      -webkit-background-size: cover;
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                    <div class="intro-message2">
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      -moz-background-size: cover;
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<h3></h3>
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      background-size: cover;
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                        <h1> I M P R O V E</h1> 
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      -o-background-size: cover;
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                    </div>
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    }
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                </div>
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    .myintro .myintro-body {
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            </div>
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      display: table-cell;
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        </div>
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    }
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        <!-- /.container -->
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    .myintro .myintro-body .brand-heading {
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      font-size: 40px;
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<div class="content-section-a">
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    }
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<div class="container">
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    .myintro .myintro-body .myintro-text {
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            <div>
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      font-size: 18px;
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                    <div class="clearfix"></div>
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      font-family: Tahoma, Geneva, sans-serif;
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                    <h2 class="section-heading"> Improved biobrick </h2>
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     }
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                    <p class="lead">
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</style>
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We have improved the function of a previous Biobrick: Original biobrick; http://parts.igem.org/Part:BBa_K864100. Our improved biobrick: http://parts.igem.org/Part:BBa_K2455002
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<div id="page-top" data-spy="scroll" data-target=".navbar-fixed-top">
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<br><br>
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The This is an improved version of the Super Yellow Fluorescent Protein 2 (BBa_K864100) carrying an N-terminal nona-arginine tag,
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and a C-terminal hexa-histidine tag. The R9-tag is a Cell-Penetrating Peptide giving it's associated protein the ability to pass through plasma membranes.
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<br><br>
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We made the improvement by inserting BBa_K864100 into the USER casette of our third biobrick, the Cell-Penetrating USER Cassette http://parts.igem.org/Part:BBa_K2455003.
 +
<br>
 +
This biobrick contains an AsiSI/Nb.BSMI USER Cassette with a N-terminal nona-arginine (R9) Cell-penetrating peptide.
 +
The nona-arginine (R9) gives any cassette inserted proteins the ability to pass through plasma membranes.
 +
In addition, the USER Cassette carries a N-terminal hexa-histidine tag which allows for easy purification.
 +
<br>
 +
For our study we demonstrated that proteins inserted into the cassette can be purified using the histidine-tag,
 +
and subsequently used to stain E. coli and Pseudomonas putida cells, presumably through internalization caused by the R9 peptide.
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</p>
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 +
</div>
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  </div>
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</div>
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 +
   
 +
<a  name="socialmedia"></a>
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        <div class="container">
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          <div class="row">
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                <div class="col-lg-5">
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                    <h2>Find Incell here:</h2>
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                </div>
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                <div class="col-lg-7">
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<br>
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                    <ul class="list-inline banner-social-buttons">
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                        <li>
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                            <a href="https://twitter.com/iGEM_Copenhagen" target="_blank" class="btn btn-default btn-lg"><i class="fa fa-twitter fa-fw"></i> <span class="network-name">Twitter</span></a>
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                        </li>
 +
                        <li>
 +
                            <a href="https://www.facebook.com/iGEMCopenhagen" target="_blank" class="btn btn-default btn-lg"><i class="fa fa-facebook fa-fw"></i> <span class="network-name">Facebook</span></a>
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                        </li>
 +
                        <li>
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                            <a href="https://www.instagram.com/igemku2017" target="_blank" class="btn btn-default btn-lg"><i class="fa fa-instagram fa-fw"></i> <span class="network-name">Instagram</span></a>
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                        </li>
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                    </ul>
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                </div>
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            </div>
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</div>
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        </div>
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        <!-- /.container -->
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    </div>
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    <!-- /.intro-header -->
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     </div>
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    <!-- /.banner -->
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    <!-- Interactive bar -->
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        <div id="page-top" data-spy="scroll" data-target=".navbar-fixed-top">
 
     <!-- One-Page Scroll NavBar -->
 
     <!-- One-Page Scroll NavBar -->
 
     <nav class="navbar scrollnav-custom navbar-fixed-bottom" role="navigation" id="scrollnav">
 
     <nav class="navbar scrollnav-custom navbar-fixed-bottom" role="navigation" id="scrollnav">
 
         <div class="container">
 
         <div class="container">
 
             <div class="navbar-header">
 
             <div class="navbar-header">
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                <button type="button" class="navbar-toggle" data-toggle="collapse" data-target=".navbar-main-collapse">
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<ul class="nav navbar-nav">
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                     Menu <i class="fa fa-bars"></i>
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                     <!-- Hidden li included to remove active class from about link when scrolled up past about section -->
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                </button>
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                    <li>
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                 <a class="navbar-brand" target="_blank"  href="https://www.facebook.com/iGEMCopenhagen">
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                 <a class="page-scroll" href="#">Top</a>
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                    <img src="https://static.igem.org/mediawiki/2017/6/67/T--BOKU-Vienna--fb2.png" alt="Facebook" >
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</li>
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                </a>
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                 </ul>
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                <a class="navbar-brand" target="_blank"  href="https://www.twitter.com/igem_copenhagen">
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                    <img src="https://static.igem.org/mediawiki/2017/1/15/T--BOKU-Vienna--twitter.png" alt="Twitter" >
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                 </a>
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             </div>
 
             </div>
  
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                     <!-- Hidden li included to remove active class from about link when scrolled up past about section -->
 
                     <!-- Hidden li included to remove active class from about link when scrolled up past about section -->
 
                     <li>
 
                     <li>
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                         <a class="page-scroll" href="#">Top</a>
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                         <a class="page-scroll" href="https://2017.igem.org/Team:UCopenhagen">Previous</a>
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                    </li>
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                    <li>
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                        <a class="page-scroll" href= "https://2017.igem.org/Team:UCopenhagen/Interdependency">Next</a>
 
                     </li>
 
                     </li>
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                 </ul>
 
                 </ul>
 
             </div>
 
             </div>
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         <!-- /.container -->
 
         <!-- /.container -->
 
     </nav>
 
     </nav>
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</div>
  
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    <!-- myintro Header -->
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     <header class="myintro">
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<!-- jQuery -->
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        <div class="myintro-body">
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     <script src="js/jquery.js"></script>
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            <div class="container">
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                <div class="row">
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    <!-- Bootstrap Core JavaScript -->
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                    <div class="col-md-10 col-md-offset-1">
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    <script src="js/bootstrap.min.js"></script>
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                        <h1 class="brand-heading">Interlab</h1>
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                        <p class="myintro-text">
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</body>
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                            <br></p>
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                        <a href="#Welcome" class="page-scroll">
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</html>
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                          <div id="downarrow">
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                            <img src="https://static.igem.org/mediawiki/2017/3/32/T--BOKU-Vienna--arrow.png" alt="V">
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                          </div>
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                        </a>
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    </header>
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Revision as of 02:52, 2 November 2017

I M P R O V E

Improved biobrick

We have improved the function of a previous Biobrick: Original biobrick; http://parts.igem.org/Part:BBa_K864100. Our improved biobrick: http://parts.igem.org/Part:BBa_K2455002

The This is an improved version of the Super Yellow Fluorescent Protein 2 (BBa_K864100) carrying an N-terminal nona-arginine tag, and a C-terminal hexa-histidine tag. The R9-tag is a Cell-Penetrating Peptide giving it's associated protein the ability to pass through plasma membranes.

We made the improvement by inserting BBa_K864100 into the USER casette of our third biobrick, the Cell-Penetrating USER Cassette http://parts.igem.org/Part:BBa_K2455003.
This biobrick contains an AsiSI/Nb.BSMI USER Cassette with a N-terminal nona-arginine (R9) Cell-penetrating peptide. The nona-arginine (R9) gives any cassette inserted proteins the ability to pass through plasma membranes. In addition, the USER Cassette carries a N-terminal hexa-histidine tag which allows for easy purification.
For our study we demonstrated that proteins inserted into the cassette can be purified using the histidine-tag, and subsequently used to stain E. coli and Pseudomonas putida cells, presumably through internalization caused by the R9 peptide.

Find Incell here: