Difference between revisions of "Team:Hamburg/Parts"

 
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                     <a href="http://parts.igem.org/Part:BBa_K2401003"style="color: grey">BBa_K2401003</a>
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                     <a href="http://parts.igem.org/Part:BBa_K2401013"style="color: grey">BBa_K2401013</a>
 
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                     <a href="http://parts.igem.org/Part:BBa_K2401013"style="color: grey">BBa_K2401013</a>
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                     <a href="http://parts.igem.org/Part:BBa_K2401003"style="color: grey">BBa_K2401003</a>
 
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<h2 class="h2">Basic Parts<h2>
 
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<table  class="table">
 
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<tr>
 
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<th>AraC family transcriptional regulator [AraCftr]</th>
 
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<th> </th>
 
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</tr>
 
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<tr>
 
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<td>Origin: </td>
 
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<td><i>Escherichia coli</i>
 
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</tr>
 
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<tr>
 
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<td>Data:</td>
 
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<td>891 bp, 296 AS, 32,3 kDa </td>
 
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</tr>
 
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<tr>
 
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<td>Part No.: </td>
 
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<td>BBa_K2401014 </td>
 
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</tr>
 
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<tr>
 
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<td>Function: </td>
 
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<td>Yersiniabactin-Complex Regulated Promotor, most likely a leaky promoter that induces the Yersiniabactin synthesis if Yersiniabactin-iron3+-complex binds to the receptor. Most siderophore receptor complexes need to be leaky since siderophores are only necessary under certain circumstances and self-induced. So, in the context of siderophores, leakiness is no flaw but a feature, including a biosensor for iron3+-ions in the expression system which makes sure that unnecessary metabolic load is exhibited. Therefore, the iron acquisition method is highly efficient and only expressed when the circumstances demand it. </td>
 
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</tr>
 
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<tr>
 
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<td>Plasmid: </td>
 
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<td>Singular on a plasmid (pSB1C3) with cAMP-resistance. </td>
 
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</tr>
 
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</table>
 
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<table class="table">
 
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<tr>
 
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<th>Regulatory protein PchR [PchR] </th>
 
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<th> </th>
 
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</tr>
 
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<tr>
 
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<td>Origin: </td>
 
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<td> <i>Pseudomonas aeruginosa PAO1</i> (strain: PAO1) </td>
 
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</tr>
 
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<tr>
 
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<td>Data: </td>
 
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<td>960 bp, 319 AS, 35.9 kDa </td>
 
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</tr>
 
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<tr>
 
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<td>Part No.:  </td>
 
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<td>BBa_K2401013 </td>
 
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</tr>
 
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<tr>
 
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<td>Function: </td>
 
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<td>Pyochelin-Complex Regulated Promotor, most likely a leaky promoter that induces the Pyochelin synthesis if Pyochelin-iron3+-di-complex binds to the receptor. Most siderophore receptor complexes need to be leaky since siderophores are only necessary under certain circumstances and self-induced. So, in the context of siderophores, leakiness is no flaw but a feature, including a biosensor for iron3+-ions in the expression system which makes sure that unnecessary metabolic load is exhibited. Therefore, the iron acquisition method is highly efficient and only expressed when the circumstances demand it.
 
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Our goal was to characterize the promoter and measure if pyochelin-gallium3+ and Nor-pyochelin-gallium3+ complexes induce the native promoter to estimate the metabolic excess overload that our antibiotic-compound induces on native <i>Pseudomonas aeruginosa</i>.
 
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</td>
 
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</tr>
 
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<tr>
 
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<td>
 
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Plasmid:
 
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</td>
 
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<td>
 
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Singular: pSB1C3 with cAMP-resistance.
 
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Combination: With HMWP2
 
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, tired, sfp and dhBe to mimic the native expression system for pyochelin to measure additional metabolic load.
 
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</td>
 
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</tr>
 
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</table>
 
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</div>
 
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-
 
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<h2 class="h2">Composite Parts<h2>
 
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    <div class="text_block text">
 
  
 
     </div>
 
     </div>

Latest revision as of 03:13, 2 November 2017

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Parts

Full Part table

 Part Number Type Designer Length
Nor Pyochelin Synthesis Cluster BBa_K2401000 Composite A. Nicolai, S. Ricke 10760 bp
Yersiniabactin inner Membrane Transporter BBa_K2401005 Composite A. Nicolai, S. Ricke 3956 bp
Siderophore outer Membrane Transporter BBa_K2401011 Composite A. Nicolai, S. Ricke 5724 bp
HMWP1 Yersiniabactin Synthetase part 2 BBa_K2401012 Composite A. Nicolai, S. Ricke 10731 bp
HMWP2 Yersiniabactin Synthetase Part 1 BBa_K2401001 Basic A. Nicolai, S. Ricke 6107 bp
2,3-Dihydroxybenzoate-AMP ligase BBa_K2401002 Basic A. Nicolai, S. Ricke 1578 bp
Thiazolinyl imide reductase BBa_K2401013 Basic A. Nicolai, S. Ricke 1101 bp
Surfactin imide reductase BBa_K2401004 Basic A. Nicolai, S. Ricke 498 bp
ABC-Siderophore-Transporter BBa_K2401006 Basic A. Nicolai, S. Ricke 1789 bp
ATP-Siderophore-Bindingpermease BBa_K2401007 Basic A. Nicolai, S. Ricke 1801 bp
HMWP1 Yersiniabactin Synthetase Part 2 BBa_K2401008 Basic A. Nicolai, S. Ricke 9492 bp
FyuA TonB Yersiniabactin outermembrane Receptor BBa_K2401010 Basic A. Nicolai, S. Ricke 2022 bp
FptA Pyochelin outermembrane Receptor BBa_K2401009 Basic A. Nicolai, S. Ricke 2160 bp
PchR Pyochelin regulator BBa_K2401003 Regulatory A. Nicolai, S. Ricke 960 bp
AraC like Yersiniabactin regulator BBa_K2401014 Regulatory A. Nicolai, S. Ricke 891 bp


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