Difference between revisions of "Team:Berlin diagnostX"
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<p class="text-justify mb-4">The colour intensity is proportional to the expression of each identified possible target.</p> | <p class="text-justify mb-4">The colour intensity is proportional to the expression of each identified possible target.</p> | ||
<img class="d-inline pfeilg mb-2" src="https://static.igem.org/mediawiki/2017/e/e4/T--Berlin_diagnostX--Pfeile.png"> | <img class="d-inline pfeilg mb-2" src="https://static.igem.org/mediawiki/2017/e/e4/T--Berlin_diagnostX--Pfeile.png"> | ||
| − | <p class="d-inline pl-3"> First direct RNA isolation | + | <p class="d-inline pl-3"> First direct RNA isolation from T. solium eggs without a manipulative hatching procedure and thereof performed transcriptome analysis </p> |
<div class="mt-5 ml-1"><a class="btn btn-lg igem_but btn-primary red" href="https://2017.igem.org/Team:Berlin_diagnostX/Design" role="button">Discover More</a></div> | <div class="mt-5 ml-1"><a class="btn btn-lg igem_but btn-primary red" href="https://2017.igem.org/Team:Berlin_diagnostX/Design" role="button">Discover More</a></div> | ||
</div> | </div> | ||
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<p class="text-justify mb-4">Based on the analysis of the build-plan of different published toehold sensors, our In Silico Team generated a first generation of switches. Further optimisation based on testing results let us to the establishment of our software for in silico design. Selected Switches were furthermore tested in the following testing pipeline.</p> | <p class="text-justify mb-4">Based on the analysis of the build-plan of different published toehold sensors, our In Silico Team generated a first generation of switches. Further optimisation based on testing results let us to the establishment of our software for in silico design. Selected Switches were furthermore tested in the following testing pipeline.</p> | ||
<img class="d-inline pfeilg mb-2" src="https://static.igem.org/mediawiki/2017/e/e4/T--Berlin_diagnostX--Pfeile.png"> | <img class="d-inline pfeilg mb-2" src="https://static.igem.org/mediawiki/2017/e/e4/T--Berlin_diagnostX--Pfeile.png"> | ||
| − | <p class="d-inline pl-3"> | + | <p class="d-inline pl-3"> Successfully established a refined preselection of promising sensors thanks to established software</p> |
<div class="mt-5 ml-1"><a class="btn btn-lg igem_but btn-primary red" href="https://2017.igem.org/Team:Berlin_diagnostX/Design" role="button">Discover More</a></div> | <div class="mt-5 ml-1"><a class="btn btn-lg igem_but btn-primary red" href="https://2017.igem.org/Team:Berlin_diagnostX/Design" role="button">Discover More</a></div> | ||
</div> | </div> | ||
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<hr class="igem_seperator mt-0 ml-0"> | <hr class="igem_seperator mt-0 ml-0"> | ||
<h5 class="igem_blue pb-1">Considerating future application from Day 1</h5> | <h5 class="igem_blue pb-1">Considerating future application from Day 1</h5> | ||
| − | <p class="text-justify mb-4"> | + | <p class="text-justify mb-4">Developing a diagnostic test needs careful consideration of the circumstances in which the toll will be used- therefore we spared no expense and investigated local circumstances in India</p> |
<img class="d-inline pfeilg mb-2" src="https://static.igem.org/mediawiki/2017/e/e4/T--Berlin_diagnostX--Pfeile.png"> | <img class="d-inline pfeilg mb-2" src="https://static.igem.org/mediawiki/2017/e/e4/T--Berlin_diagnostX--Pfeile.png"> | ||
| − | <p class="d-inline pl-3"> | + | <p class="d-inline pl-3"> Find out more about what the insight we gained in India!</p> |
<div class="mt-5 ml-1"><a class="btn btn-lg igem_but btn-primary red" href="https://2017.igem.org/Team:Berlin_diagnostX/HP/Gold_Integrated" role="button">Discover More</a></div> | <div class="mt-5 ml-1"><a class="btn btn-lg igem_but btn-primary red" href="https://2017.igem.org/Team:Berlin_diagnostX/HP/Gold_Integrated" role="button">Discover More</a></div> | ||
</div> | </div> | ||
Revision as of 03:35, 2 November 2017
The fight against T. solium
The worldwide fight against Taenia solium (pork tapeworm) is facing three different challenges
HIGH PREVALENCE
Estimated billions are affected, the infection causing epilepsy, blindness and death
INSUFFICIENT DRUG DISTRIBUTION
Although affordable therapeutics exit
EXPENSIVE DIAGNOSTICS
No method suited for field use
The vision of diagnost-x
Developing a low cost, easy-to-handle diagnostic test for infection with the tapeworm Taenia solium
Target Identification
Having 96 million RNAs sequenced and mapped them against about 12.000 Genes of T. saginata T. asiatica and T. solium, the expression level of unique RNAs was analyzed. We identified 215 unique, potential diagnost-x targets.
The colour intensity is proportional to the expression of each identified possible target.
First direct RNA isolation from T. solium eggs without a manipulative hatching procedure and thereof performed transcriptome analysis
Sensor Design
Based on the analysis of the build-plan of different published toehold sensors, our In Silico Team generated a first generation of switches. Further optimisation based on testing results let us to the establishment of our software for in silico design. Selected Switches were furthermore tested in the following testing pipeline.
Successfully established a refined preselection of promising sensors thanks to established software
Sensor Screening
The designed switches from the In Silico Team were furthermore assembled using Extension PCR with for every switch tailored primers. Assembled Sensors passed all through the standard screening in liquid phase.
6 successfully working sensors detected thanks to our high flow-through Screening Pipeline
Considerating future application from Day 1
Developing a diagnostic test needs careful consideration of the circumstances in which the toll will be used- therefore we spared no expense and investigated local circumstances in India
Find out more about what the insight we gained in India!
Raising awareness for T. solium
Neglected Tropical Diseases have few platforms in the eastern world since they don't affect people directly. We saw our mission also to a big extend in the fundamental raising of awareness for NTDs and education of a new generation.
Organisation of a congress with steakholders & 150 participants amongst many other activities
Route to the Future
The 21st century leaves it's marks - new methods for intelligent assisted education are wanted - and we're on the track of time! Dynamic & new ways of solving problems will determine the success of social entrepreneurship - get started!
Development of an augmented reality software tool for innovative future education
Many Thanks to our Sponsors!
