Difference between revisions of "Team:Lund/Design/Animation"
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<h3>The working system</h3> | <h3>The working system</h3> | ||
| − | <p>In presence of a phthalate molecule, which binds to hER-α LBD, the truncated receptor undergoes a conformational change which | + | <p>In presence of a phthalate molecule, which binds to hER-α LBD, the truncated receptor undergoes a conformational change which brings the GFP10 and GFP11 tags closer together.</p> |
</div> | </div> | ||
</section> | </section> | ||
Revision as of 16:23, 2 December 2017
Design
Our sensory elements
Superfolder GFP was split into three parts, namely GFP1-9 (the leftmost figure), GFP10 and GFP11. The GFP1-9 protein will be expressed in presence of organic pollutant molecules. The GFP10 and GFP11 tags were fused to the ligand binding domain of the human estrogen receptor alpha (hER-α LBD).
The working system
In presence of a phthalate molecule, which binds to hER-α LBD, the truncated receptor undergoes a conformational change which brings the GFP10 and GFP11 tags closer together.
Let there be light
Expressed GFP1-9 (indication of OP presence) binds to the GFP10-GFP11 construct (indicating of phthalate presence) and reconstitutes a functional sfGFP, resulting in a measurable fluorescent signal.
