Difference between revisions of "Team:SJTU-BioX-Shanghai/Basic Part"

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                         <div class="my-title h5-my-responsive">Introduction</div>
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                         <div class="my-title h5-my-responsive">总体介绍</div>
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                         <p>This year, our team will choose to present STAR 3, another modified STAR (Small Transcriptional-Activating RNA) for the award of the basic part (<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2285020">BBa_K2285020</a>).
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                         <p>今年我们队伍将选择另一个修改过的STAR——STAR3作为今年的基本模块(<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2285020">BBa_K2285020</a>)。
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                             Our project was based on iGEM16_Imperial STAR system (to distinguish it from our work, we called it <strong>STAR 1 system</strong>), and we decided to build another STAR system (we called it <strong>STAR 3 system</strong>)
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                             我们今年的项目是建立在2016年帝国理工学校STAR系统的基础的（为了区分我们和他们的工作，我们把他们的STAR系统称为STAR1），然后我们决定建立另一套STAR系统（我们称之为STAR3）来达到多因子检测的目的。简单来说就是两套STAR系统可以控制两个基因回路。更多关于STAR优势和工作原理的信息可以在
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                            to achieve multiple responds to different conditions. To put it simply, two STAR systems together can control two genetic circuits. For more information about advantages of STAR and how it works, you can be refer to <a target="_blank"
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                        <a target="_blank"
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                                 href="https://2016.igem.org/Team:Imperial_College/Basic_Part">here</a> .</p>
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                                 href="https://2016.igem.org/Team:Imperial_College/Basic_Part">这里</a>查阅。</p>
 
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                                 which is the antisense of part of the target RNA, is transcribed, the terminator structure will be disrupted and switch on the inhibited transcription.</div>
 
                                 which is the antisense of part of the target RNA, is transcribed, the terminator structure will be disrupted and switch on the inhibited transcription.</div>
 
                         </div>
 
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                         <p class="my-title2">For convenience, we want to introduce some terms for later description:</p>
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                         <p class="my-title2">为了之后描述的方便，我们在这里引入一些名词用于指代各个原件:</p>
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                         <p>In the original project of Imperial, the stem-loop structure, which could be seen as a terminator, on the upstream of functional genes is called STAR-Target and we would just name it <strong>Target</strong>. The DNA sequence that
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                         <p>在帝国理工的项目里，基因上游的茎环结构被称为STAR-Target，我们简略称为Target，其互补序列我们称之为Antisense。当我们提到Target1和Antisense1的时候，我们指的是帝国理工构建的那套系统。当我们提到Target3和Antisense3的时候，我们说的是我们今年项目构建的系统。</p>
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                            transcribes STAR we rename it <strong>Antisense</strong>. When we say <strong>Target 1</strong> and <strong>Antisense 1</strong>, they refer to the terminator structure and its complementary sequence in Imperial’s project.
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                         <p>请浏览我们的 <a target="_blank" href="https://2017.igem.org/Team:SJTU-BioX-Shanghai/Design">设计页面</a>来获得更多关于STAR3系统设计的信息</p>
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                            When we say <strong>Target 3</strong> and <strong>Antisense 3</strong>, they refer to the novel terminator structure and its complementary sequence we designed this year. Target 1 and Antisense 1 together constitute STAR 1
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                         <p>另外可以浏览 Target 3 <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2285020">BBa_K2285020</a> 原件页面来获取更多关于STAR系统表征的信息 (Both STAR 1 and STAR 3) system.</p>
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                            system and Target 3 and Antisense 3 constitute STAR 3 system.</p>
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                         <p>Please visit our <a target="_blank" href="https://2017.igem.org/Team:SJTU-BioX-Shanghai/Design">Design page</a> to find more information about how we designed the STAR 3 system.</p>
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                         <p>Alternatively check out Target 3 <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2285020">BBa_K2285020</a> webpage for characterization of the STARs (Both STAR 1 and STAR 3) system.</p>
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                         <div class="my-title h5-my-responsive">Basic Parts we submitted</div>
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                         <div class="my-title h5-my-responsive">我们提交的基本模块</div>
 
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Revision as of 12:06, 9 December 2017

Basic Parts
总体介绍

今年我们队伍将选择另一个修改过的STAR——STAR3作为今年的基本模块(BBa_K2285020)。 我们今年的项目是建立在2016年帝国理工学校STAR系统的基础的（为了区分我们和他们的工作，我们把他们的STAR系统称为STAR1），然后我们决定建立另一套STAR系统（我们称之为STAR3）来达到多因子检测的目的。简单来说就是两套STAR系统可以控制两个基因回路。更多关于STAR优势和工作原理的信息可以在 这里查阅。

Figure 1. The STAR construct. In the absence of antisense, sense target RNA will form a stem-loop structure, functioning as a terminator and stop the following transcription. When antisense RNA, a mRNA fragment which is the antisense of part of the target RNA, is transcribed, the terminator structure will be disrupted and switch on the inhibited transcription.

为了之后描述的方便，我们在这里引入一些名词用于指代各个原件:

在帝国理工的项目里，基因上游的茎环结构被称为STAR-Target，我们简略称为Target，其互补序列我们称之为Antisense。当我们提到Target1和Antisense1的时候，我们指的是帝国理工构建的那套系统。当我们提到Target3和Antisense3的时候，我们说的是我们今年项目构建的系统。

请浏览我们的 设计页面来获得更多关于STAR3系统设计的信息

另外可以浏览 Target 3 BBa_K2285020 原件页面来获取更多关于STAR系统表征的信息 (Both STAR 1 and STAR 3) system.

我们提交的基本模块
Part Number Type Description Length (bp)
BBa_K2285010 RNA Antisense3+t500 104
BBa_K2285020 Regulatory J23119+Target3 128
Reference
  1. Chappell J, Takahashi MK, Lucks JB. 2015. Creating small transcription activating RNAs. Nat Chem Biol 11:214–220.
  2. Meyer, S., Chappell, J., Sankar, S., Chew, R., and Lucks, J. B. (2016) Improving fold activation of small transcription activating RNAs (STARs) with rational RNA engineering strategies Biotechnol. Bioeng. 113, 216.
Wet Lab
Protocol
Wet Lab
Composite Parts