Difference between revisions of "Team:FAFU-CHINA"
XiaojiangLi (Talk | contribs) |
XiaojiangLi (Talk | contribs) |
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margin: 0 auto; | margin: 0 auto; | ||
height:100%; | height:100%; | ||
| − | background:url( | + | background:url(image/T--FAFU-CHINA--12.1.png) scroll center; |
background-repeat:no-repeat; | background-repeat:no-repeat; | ||
background-size:100%; | background-size:100%; | ||
| Line 39: | Line 39: | ||
} | } | ||
| − | . | + | |
| + | |||
| + | .menuss, .menuss ul { | ||
list-style: none; | list-style: none; | ||
padding: 0; | padding: 0; | ||
| Line 45: | Line 47: | ||
} | } | ||
| − | . | + | .menuss { |
height: 58px; | height: 58px; | ||
margin-top: 20px; | margin-top: 20px; | ||
} | } | ||
| − | . | + | .menuss li { |
background-color: #0C0000; | background-color: #0C0000; | ||
border-bottom: 2px solid #181818; | border-bottom: 2px solid #181818; | ||
| Line 57: | Line 59: | ||
} | } | ||
| − | . | + | .menuss > li { |
display: block; | display: block; | ||
float: left; | float: left; | ||
| Line 65: | Line 67: | ||
| − | . | + | .menuss > li:first-child { |
border-radius: 25px 0 0 0; | border-radius: 25px 0 0 0; | ||
} | } | ||
| − | . | + | .menuss > li:last-child { |
border-radius: 0 25px 0 0; | border-radius: 0 25px 0 0; | ||
} | } | ||
| Line 74: | Line 76: | ||
| − | . | + | .menuss a { |
border-left: 3px solid rgba(0, 0, 0, 0); | border-left: 3px solid rgba(0, 0, 0, 0); | ||
/*background: url("../img/back.jpg");*/ | /*background: url("../img/back.jpg");*/ | ||
| Line 86: | Line 88: | ||
} | } | ||
| − | . | + | .menuss li:hover { |
background-color: #1c1c1c; | background-color: #1c1c1c; | ||
background: -moz-linear-gradient(#1c1c1c, #1b1b1b); | background: -moz-linear-gradient(#1c1c1c, #1b1b1b); | ||
| Line 107: | Line 109: | ||
color: #C4302B; | color: #C4302B; | ||
} | } | ||
| − | . | + | .menuss li:hover > a { |
border-radius: 5px 0 0 0; | border-radius: 5px 0 0 0; | ||
border-left: 3px solid #C4302B; | border-left: 3px solid #C4302B; | ||
| Line 113: | Line 115: | ||
} | } | ||
| − | . | + | .submenuss { |
left: 0; | left: 0; | ||
max-height: 0; | max-height: 0; | ||
| Line 126: | Line 128: | ||
} | } | ||
| − | . | + | .submenuss li { |
opacity: 0; | opacity: 0; | ||
-webkit-transform: rotateY(90deg); | -webkit-transform: rotateY(90deg); | ||
| Line 141: | Line 143: | ||
} | } | ||
| − | . | + | .menuss .submenuss li:hover a { |
border-left: 3px solid #454545; | border-left: 3px solid #454545; | ||
border-radius: 0; | border-radius: 0; | ||
| Line 147: | Line 149: | ||
} | } | ||
| − | . | + | .menuss > li:hover .submenuss, .menuss > li:focus .submenuss { |
max-height: 2000px; | max-height: 2000px; | ||
z-index: 10; | z-index: 10; | ||
} | } | ||
| − | . | + | .menuss > li:hover .submenuss li, .menuss > li:focus .submenuss li { |
opacity: 1; | opacity: 1; | ||
-webkit-transform: none; | -webkit-transform: none; | ||
| Line 161: | Line 163: | ||
} | } | ||
/*动画递进的时间 实现一个个螺旋子菜单*/ | /*动画递进的时间 实现一个个螺旋子菜单*/ | ||
| − | . | + | .menuss li:hover .submenuss li:nth-child(1) { |
-webkit-transition-delay: 0s; | -webkit-transition-delay: 0s; | ||
-moz-transition-delay: 0s; | -moz-transition-delay: 0s; | ||
| Line 169: | Line 171: | ||
} | } | ||
| − | . | + | .menuss li:hover .submenuss li:nth-child(2) { |
-webkit-transition-delay: 50ms; | -webkit-transition-delay: 50ms; | ||
-moz-transition-delay: 50ms; | -moz-transition-delay: 50ms; | ||
| Line 177: | Line 179: | ||
} | } | ||
| − | . | + | .menuss li:hover .submenuss li:nth-child(3) { |
-webkit-transition-delay: 100ms; | -webkit-transition-delay: 100ms; | ||
-moz-transition-delay: 100ms; | -moz-transition-delay: 100ms; | ||
| Line 185: | Line 187: | ||
} | } | ||
| − | . | + | .menuss li:hover .submenuss li:nth-child(4) { |
-webkit-transition-delay: 150ms; | -webkit-transition-delay: 150ms; | ||
-moz-transition-delay: 150ms; | -moz-transition-delay: 150ms; | ||
| Line 193: | Line 195: | ||
} | } | ||
| − | . | + | .menuss li:hover .submenuss li:nth-child(5) { |
-webkit-transition-delay: 200ms; | -webkit-transition-delay: 200ms; | ||
-moz-transition-delay: 200ms; | -moz-transition-delay: 200ms; | ||
| Line 201: | Line 203: | ||
} | } | ||
| − | . | + | .menuss li:hover .submenuss li:nth-child(6) { |
-webkit-transition-delay: 250ms; | -webkit-transition-delay: 250ms; | ||
-moz-transition-delay: 250ms; | -moz-transition-delay: 250ms; | ||
| Line 209: | Line 211: | ||
} | } | ||
| − | . | + | .menuss li:hover .submenuss li:nth-child(7) { |
-webkit-transition-delay: 300ms; | -webkit-transition-delay: 300ms; | ||
-moz-transition-delay: 300ms; | -moz-transition-delay: 300ms; | ||
| Line 217: | Line 219: | ||
} | } | ||
| − | . | + | .menuss li:hover .submenuss li:nth-child(8) { |
-webkit-transition-delay: 350ms; | -webkit-transition-delay: 350ms; | ||
-moz-transition-delay: 350ms; | -moz-transition-delay: 350ms; | ||
| Line 225: | Line 227: | ||
} | } | ||
| − | . | + | .submenuss li:nth-child(1) { |
-webkit-transition-delay: 350ms; | -webkit-transition-delay: 350ms; | ||
-moz-transition-delay: 350ms; | -moz-transition-delay: 350ms; | ||
| Line 233: | Line 235: | ||
} | } | ||
| − | . | + | .submenuss li:nth-child(2) { |
-webkit-transition-delay: 300ms; | -webkit-transition-delay: 300ms; | ||
-moz-transition-delay: 300ms; | -moz-transition-delay: 300ms; | ||
| Line 241: | Line 243: | ||
} | } | ||
| − | . | + | .submenuss li:nth-child(3) { |
-webkit-transition-delay: 250ms; | -webkit-transition-delay: 250ms; | ||
-moz-transition-delay: 250ms; | -moz-transition-delay: 250ms; | ||
| Line 249: | Line 251: | ||
} | } | ||
| − | . | + | .submenuss li:nth-child(4) { |
-webkit-transition-delay: 200ms; | -webkit-transition-delay: 200ms; | ||
-moz-transition-delay: 200ms; | -moz-transition-delay: 200ms; | ||
| Line 257: | Line 259: | ||
} | } | ||
| − | . | + | .submenuss li:nth-child(5) { |
-webkit-transition-delay: 150ms; | -webkit-transition-delay: 150ms; | ||
-moz-transition-delay: 150ms; | -moz-transition-delay: 150ms; | ||
| Line 265: | Line 267: | ||
} | } | ||
| − | . | + | .submenuss li:nth-child(6) { |
-webkit-transition-delay: 100ms; | -webkit-transition-delay: 100ms; | ||
-moz-transition-delay: 100ms; | -moz-transition-delay: 100ms; | ||
| Line 273: | Line 275: | ||
} | } | ||
| − | . | + | .submenuss li:nth-child(7) { |
-webkit-transition-delay: 50ms; | -webkit-transition-delay: 50ms; | ||
-moz-transition-delay: 50ms; | -moz-transition-delay: 50ms; | ||
| Line 281: | Line 283: | ||
} | } | ||
| − | . | + | .submenuss li:nth-child(8) { |
-webkit-transition-delay: 0s; | -webkit-transition-delay: 0s; | ||
-moz-transition-delay: 0s; | -moz-transition-delay: 0s; | ||
| Line 291: | Line 293: | ||
</style> | </style> | ||
| − | + | <div class="nav"> | |
| − | <ul class=" | + | <ul class="menuss"> |
<li class="xyz"><a href="https://2017.igem.org/Team:FAFU-CHINA">HOME</a></li> | <li class="xyz"><a href="https://2017.igem.org/Team:FAFU-CHINA">HOME</a></li> | ||
<li><a href="javascript:;">PROJECT</a> | <li><a href="javascript:;">PROJECT</a> | ||
| − | <ul class=" | + | <ul class="submenuss"> |
<li><a href="https://2017.igem.org/Team:FAFU-CHINA/Description">Description</a></li> | <li><a href="https://2017.igem.org/Team:FAFU-CHINA/Description">Description</a></li> | ||
<li><a href="https://2017.igem.org/Team:FAFU-CHINA/Experiments">Experiments</a></li> | <li><a href="https://2017.igem.org/Team:FAFU-CHINA/Experiments">Experiments</a></li> | ||
| Line 308: | Line 310: | ||
<li><a href="https://2017.igem.org/Team:FAFU-CHINA/Team">Team</a></li> | <li><a href="https://2017.igem.org/Team:FAFU-CHINA/Team">Team</a></li> | ||
</ul> | </ul> | ||
| − | + | </div> | |
</div> | </div> | ||
Revision as of 08:57, 11 August 2017
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Continued worldwide industrialization has caused extensive environmental and human health problems. A wide variety of chemicals, e.g., heavy metals, pesticides, chlorinated solvents, etc., have been detected in different natural resources such as soil, water, and air. Among the pollutants, the heavy metals are of concern to human health due to their cytotoxicity,mutagenicity, and carcinogenicity.
Soil polluted by heavy metals represent an important environmental problem due to the toxic effects of metals, their accumulations throughout the food chain and the additional risk of groundwater contamination.
Metal remediation through common physico-chemical techniques is expensive and unsuitable in the case of extensive areas. Therefore biotechnological approaches have received a great deal of attention in recent years. Bioremediaton, as a way of natural attenuation, adds phytoremediation, the use of plants for reclamation and microbe(PSB) together as a strategy for either enhancing uptake(PSB-assisted Phytoextraction) or reducing the bioavailability of metal contaminants(PSB-immobilized Phyto-promotion mechanism) in the soil, especially in the rhizosphere to accelerate plant biomass production and root enlongation.overvie
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Phytoextraction is based on the use of hyperaccumulator plants, which can tolerate and accumulate high concentration of metals. Ideal hyperaccumulators require the characteristics of deep rooted, rapid growth and a high amount of biomass. In fact, many hyperaccumulators are slow in growth, produce low bow biomass and cannot grow in metal-stress soil. Further, the overproduction of ethylene in response to metal-stress leads to inhibition of root growth, root hair formation and consequently development of the plant as a whole. Therefore, in our project we took advantage of co-expression of ACC deaminase and iaaM genes in our chassis PSB(B. megaterium) to enhance plant growth and biomass by P solubilization and uptake particularly under stress condition by heavy metals. Meanwhile, we also exploited bacterial chemotaxis, McfS, a multiple chemoreceptor from strain P. putida F1 to detect response of organic acids secreting in the rhizosphere.
PSB-assisted Phytoextraction(ex situ)
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In this module, our team designed a pathway that the Pb ions will bind to PbR protein when activated ions are in the soil environment. It induces downstream gene expression, and results in the tetR protein inhibition promoter PltetO1 expression. At the same time, the strong promoter P43 of B.megaterium stimulated the expression of MBP in the downstream lead-producing peptide. It makes the chelating peptide of Pb metal, which reduced the total amount of heavy metals in the soil to be harmless level. When there is no Pb ions presented in the environment, the expression of tetR was blocked and the inhibition of PltetO1 was released. It results in the lacI repressor binding to the lacO sequence, and leads to blocking the expression of MBP. This mechanism is effective in preventing the infinite expression of MBP against the B. megaterium itself.
PSB-immobilized Phyto-promotion
