Team:UNOTT/Modelling
Modeling
The central aim for the modeling would be to write a simulation for the wet lab to aid construction of the bacteria.
- Predicting Fluoresce Intensity and Wavelengths
- MISSION: SYNTHESISING BRICKS
- MISSION: MUTATING DCAS9
- MISSION: LIGATING BRICKS
- MISSION: CONTROL PLASMID TRANSFORMATION
-
Predicting Fluoresce Intensity and Wavelengths Given Parameters
Constitutive Gene Expression For Protein and mRNA Expression over Time
Assuming the gene expression was unregulated and the gene is always on, the constitutive gene expression model was used to guide the model.
$$ sfGFP \underset{Transcriptin}{\rightarrow} mRNA \underset{Translation}{\rightarrow} sfGFP $$ $$ mRNA \underset{Degradation}{\rightarrow} \oslash $$ $$ sfGFP \underset{Degradation}{\rightarrow} \oslash $$Using the Law of Mass Action, this model can be described as:
$$ mRNA = k_{1} -d _{1 } mRNA $$ $$ Protein = k_{2} \cdot mRNA - d_{2} \cdot Protein $$Using this, we can calculate the concentration of proteins we can expect over time. This was useful because it allowed us to see how much protein was made which were emitting the light so we could make an accurate prediction of how much intensity there was at a certain amount of time.
Text
MISSION: SYNTHESISING BRICKS
Text
MISSION: MUTATING DCAS9
Text
MISSION: LIGATING BRICKS
Text
MISSION: CONTROL PLASMID TRANSFORMATION
Text
