Team:Bielefeld-CeBiTec/Results/toolbox/photoswitching

• Aim of the Project
• Unnatural Base Pair ▼
• Translational System ▼
• Toolbox ▼

• Overview
• Unnatural Base Pairs
• Uptake and Biosynthesis
• Retention System
• Challenges

• Overview
• Translation Mechanism
• Library and Selection

• Overview
• Analyzing
• Photoswitching
• Labeling
• Photolysis
• Fusing

• Results Overview
• Unnatural Base Pair ▼
• Translational System ▼
• Toolbox ▼
• Parts ▼
• Achievements

• Overview
• Uptake
• Biosynthesis
• Retention System
• Development of New Methods

• Overview
• Translation Mechanism
• Library and Selection

• Overview
• Analyzing
• Photoswitching
• Labeling
• Photolysis
• Fusing

• Parts
• Basic Parts
• Composite Parts
• Part Collection
• Improve

• Hardware
• Software
• Modeling
• Measurement
• Applied Design
• InterLab Study

• Overview
• Gold
• Silver
• Public Engagement

• Members
• Advisors
• Bielefeld
• CeBiTec
• Bielefeld University
• Attributions

• Methods
• Oligonucleotides
• Labjournal
• Labnotes Generator
• Safety

Photoswitching

Design of AzoF-RS

The AzoF-RS was provided by CU Boulder and deposited in the Registry of Standard Biological Parts as BBa_X. They got it from the Schultz lab, which performed a selection experiment on the M. jannaschii TyrRS to evolve a new aaRS capable of incorporating the photoisomerizable phenylalanine-4‘-azobenzene (AzoF). Figure 1 shows a sequence alignment of the protein sequences of the M. jannaschii TyrRS and the AzoF-RS after the selection process.

Figure 1: Sequence alignment of the M. jannaschii TyrRS and the AzoF-RS of the Schultz lab. The alignment shows six differences in the protein sequences.