Team:UCSC/Safety
SAFETY
When choosing an organism to help us produce acetaminophen and vitamin B-12 we sought out an FDA approved microbe; however, we resorted to using a non-toxic cyanobacteria as a model for spirulina. Spirulina is a generally recognized as safe organism and our genes were chosen from bio-safety level 1 organisms.
LAB SAFETY
Proper PPE for bio-safety level 1 organisms are worn in our labratory. Within the lab space, every person is expected to be wearing gloves, a labcoat, and protective goggles. All personnel also must be accompanied with one other person so there is no one alone in lab.
PROJECT SAFETY
Vitamin B-12 Safety
GENES:
I. ssuE => produces FMN reductase
The synthesis of 5,6-DMB first requires the presence of reduced flavin mononucleotide (FMNH2). FMNH2 is reduced from flavin mononucleotide (FMN) by FMN reductase, which is encoded with the ssuE gene [19, 20].
organism: synechoccocous 7002
FMNH2 + NADP+ = FMN + NADPH
II. Gene bluB => produces
