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Dry Lab
- The first week involved learning all about synthetic biology and what lies ahead in terms of the competition
- Dr Louise Brown and explained all things iGEM including the biobrick system and 3A assembly
- An overview of the chlorophyll biosynthesis pathway, photosystem II and hydrogenase activity presented by Professor Robert Willows since our project would focus on one component of this bigger picture
- Our project being to use biosynthetic techniques to create the hydrogen producing hydrogenase
- We started planning for creation of complete hydrogenase plasmid by combining the Ferredoxin/Ferredoxin reductase biobrick with the Hyd1 biobrick
- From this we expect some preliminary production of hydrogen however, with the addition of maturation enzymes this production would be maximised.
- Discussion on construction of the maturation plasmid in which HydE/HydF biobrick would be combined with HydG biobrick then the two larger constructs would be ligated together to create the complete plasmid coding for the total Hydrogenase molecular machine, fondly named Omega Ω
- We created a “3-day plan” from 3A assembly through transformation and plating up cells to liquid media overnight cultures, miniprep kit extraction of plasmid and nanodrop concentration measurement, and finally electrophoresis gel screening of our newly made construct
- Discussion on human outreach was started.
- Began talking to the SDU iGEM team on collaboration
- Discussed the creation of an iGEM Macquarie game
- Started planning for attending synthetic biology conference and ACUR (Adelaide).
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