Team:TP-CC San Diego/LabBook
Lab Notebook
DAY 1
SUMMARY of July 17th:
- DNA extraction from GBM39 cells
- Agarose gel electrophoresis to confirm DNA
DAY 2
SUMMARY of July 18th:
- qPCR primer design and order
DAY 3
SUMMARY of July 21:
- Primers arrived
DAY 4
SUMMARY of August 1st:
DAY 5
SUMMARY of August 2nd:
- Received oligos
DAY 6
SUMMARY of August 7th:
- Digestion of lentiviral CRISPR plasmid
- Gel purification digested plasmid using QIAquick Gel Extraction Kit and eluted in buffer EB
- There should be two bands on the gel, one at 2kb which should not be purified. Purify only the larger band
DAY 7
SUMMARY of August 9th:
- Anneal oligo pairs
- Ligation and transformation
DAY 8
SUMMARY of August 10th:
- No colonies grew on carb antibiotic plates
