Team:TP-CC San Diego/Protocols


Protocols

TRANSFORMATION PROTOCOL

Procedure:

  1. Thaw comp cells on ice for 10 mins.
  2. Add 10uL of DNA to comp cells.
  3. Let sit on ice for 10 mins
  4. Heat shock at 42˚C for 1 min
  5. Let sit on ice for 2 min
  6. Add 300uL of SOC media
  7. Put in 37˚C shaker at 200 rpm for 1 hr
  8. Place agar plates in 37˚C incubator to warm up; remove when needed.
  9. Pipette 300uL of comp cells onto agar plate
  10. Use sterilized beads to spread comp cells evenly
  11. Place in 37˚C incubator overnight