Team:IISc-Bangalore/Protocols

  1. Transformation
  2. Plasmid isolation
  3. PCR
  4. DNA purification
  5. Restriction digestion
  6. Ligation
  7. SEM assay
  8. DLS assay
  9. Spectrophotometry assay

Transformation

Preparation of competent cells

TSS method

CaCl2 method

Heat-shock transformation

Plasmid isolation

Miniprep

Midiprep

PCR

Simple PCR

Overhang PCR

Colony PCR

DNA purification

Chloroform extraction

Gel purification

Restriction digestion

Single digestion

Double digestion

Overnight digestion

Ligation

Simple ligation

Sequential ligation

Multi-ligation

Scanning Electron Microscopy (SEM) assay

Dynamic Light Scattering (DLS) assay

Spectrophotometry assay