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June 2017 | ||||||
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September 2017 | ||||||
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October 2017 | ||||||
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Micah, Maddie, Alex
- Prepared LB Media
- Re-suspended DNA (D. Radiodurans Uracil DNA Glycosylate 2) from iGEM kit
- Transformed Uracil DNA Glycosylate 2 in psB1C3 plasmid into MHD42 competent cells (Practice transformation)
- Prepared LB Agar
- Prepared overnight culture to make competent cells (MHD42)
Micah, Maddie
- Prepared MHD42 competent cells
- Checked on transformed MHD42 cells with Uracil DNA Glycosylate 2
- Transformation was a success with 100 colonies
- Plate was stored in 4 deg. Celsius for future use
- Prepared TSS Buffer
No Work!
No Work!
Micah, Maddie, Alex, Mark, Collin
- Prepared Chloramphenicol (CM) plates
- Prepared Ampicillin solution to be used for plates in the future
- Made more LB Agar
Micah, Maddie, Alex, Mark, Collin
- Prepared DH5α competent cells
- Tested MHD42 cells for competency
- Tested DH5α cells for competency