Team:Gaston Day School/Notebook
Notebook
On this page, Gaston Day School iGEM team would like to share with you our notes during the experiments from 09/01/2017 to 10/23/2017. The team leader Heena Saqib records the data patiently.
| Step | Temperature (°C) | Time |
|---|---|---|
| Initial denaturation | 98 | 30 seconds |
| 25-35 cycles | 98 (denaturation) 45-72 (annealing) see Note 1 72 (extension) |
5-10 seconds 10-30 seconds 15-30 seconds per kb |
| Final extension | 72 | 2-5 minutes |
| Hold | 4 | Indefinitely |
Note 1: Use the NEB Tm calculator should be used to determine the annealing temperature when using Phusion: http://tmcalculator.neb.com/#!/
