Team:IISc-Bangalore/Design

  1. T7 promoter
  2. T7 terminator
  3. sfGFP
  4. SpyCatcher
  5. mCherry
  6. T7 expression system
  7. sfGFP-SpyCatcher fusion
  8. mCherry-SpyTag fusion

T7 promoter (BBa_K525998)

T7 terminator (BBa_K731721)

sfGFP (BBa_K1321337)

SpyCatcher (BBa_K1650037)

mCherry (BBa_J18932)

Building a modular T7 expression system

sfGFP-SpyCatcher fusion protein

6xHis-mCherry-SpyTag fusion protein