Oligonucleotides
ID | Name | Length | Tm °C | Sequence 5'-> 3' |
---|---|---|---|---|
17aa | tetM_gaF | 48 | 61.0 | ACAATGCTAGCGACAAAGAGGAGAAAGTAATGCGCACCCTGAACATCG |
17ab | tetM_gaR | 49 | 60.0 | TTTGCCGGACTGCAGCGGCCGCTACTAGTACTACTCACCCGAATGCCCG |
17ac | tetM_gaF | 60 | 56.0 | ACAATGCTAGCGACAAAGAGGAGAAAGTAATGAAAATCATAAATATCGGTGTATTAGCTC |
17ad | tetM_gaR | 60 | 55.0 | TTTGCCGGACTGCAGCGGCCGCTACTAGTATTATGTGATTTTGTTGAACATATAACGAAC |
17ae | tetM_gaF | 64 | 56.0 | TACAATGCTAGCGACAAAGAGGAGAAAGTAATGAAAATTATTAATATTGGAGTTTTAGCTCATG |
17af | tetM_gaR | 74 | 55.0 | TTTGCCGGACTGCAGCGGCCGCTACTAGTATTATTAAGTTATTTTATTGAACATATATCTTACTTTATCTATCC |
17ag | pSB1C3-mRFP_gaF | 49 | 68.0 | CTAGTGTAGATCGCGGTCTCATGCGTACTAGTAGCGGCCGCTGCAGTCC |
17ah | pSB1C3-mRFP_gaR | 73 | 68.0 | CTCCTCTTTGTCGCTAGCATTGTACCTAGGACTGAGCTAGCCATAAACTCTAGAAGCGGCCGCGAATTCCAGA |
17ai | aac2_gaF | 49 | 62.0 | TACAATGCTAGCGACAAAGAGGAGAAAGTAATGACCATCGACCTCCGCC |
17aj | aac2_gaR | 48 | 62.0 | TTTGCCGGACTGCAGCGGCCGCTACTAGTATCAGGTGACGTCGCCGTC |
17ak | mRFP_gaF | 68 | 58.0 | CTAGGTACAATGCTAGCGACAAAGAGGAGAAAGTAATGGGCGAGACCGCTTCCTCCGAAGACGTTATC |
17al | mRFP_gaR | 65 | 58.0 | TTTTGCCGGACTGCAGCGGCCGCTACTAGTACGCATGAGACCGCGATCTACACTAGCACTATCAG |
17am | aadA_gaF | 49 | 60.0 | TACAATGCTAGCGACAAAGAGGAGAAAGTAATGAGGGAAGCGGTGATCG |
17an | aadA_gaR | 58 | 59.0 | TTTGCCGGACTGCAGCGGCCGCTACTAGTATTATTTGCCGACTACCTTGGTGATCTCG |
17ao | ermc_gaF | 62 | 57.0 | ACAATGCTAGCGACAAAGAGGAGAAAGTAATGAACGAGAAAAATATAAAACACAGTCAAAAC |
17ap | ermc_gaR | 78 | 58.0 | TTTGCCGGACTGCAGCGGCCGCTACTAGTATTACTTATTAAATAATTTATAGCTATTGAAAAGAGATAAGAATTGTTC |
17aq | msrA_gaF | 67 | 58.0 | ACAATGCTAGCGACAAAGAGGAGAAAGTAATGGAACAATATACAATTAAATTTAACCAAATCAATCA |
17ar | msrA_gaR | 67 | 58.0 | TTTGCCGGACTGCAGCGGCCGCTACTAGTATTAAGTTATATCATGAATAGATTGTCCTGTTAATTCC |
17as | mef_gaF | 62 | 58.0 | ACAATGCTAGCGACAAAGAGGAGAAAGTAATGGAAAAATACAACAATTGGAAACTTAAGTTT |
17at | mef_gaR | 71 | 58.0 | TTTGCCGGACTGCAGCGGCCGCTACTAGTATTATTTTAAATCTAATTTTCTAATCTCATTTATCATTGGGC |
17au | ermX_gaF | 48 | 58.0 | ACAATGCTAGCGACAAAGAGGAGAAAGTAATGTCTGCATACGGACACG |
17av | ermX_gaR | 51 | 58.0 | TTTGCCGGACTGCAGCGGCCGCTACTAGTATCACCTGGAAGAGATCGATCC |
17aw | carB4_gaF | 54 | 60.0 | TACAATGCTAGCGACAAAGAGGAGAAAGTAATGAAGTTGCTGTTAGTGTTCTCG |
17ax | carB4_gar | 53 | 59.0 | TTTGCCGGACTGCAGCGGCCGCTACTAGTATCAACGGCTTTGCGAACTATAAA |
17ay | tetX_gaF | 47 | 61.0 | TGCTAGCGACAAAGAGGAGAAAGTAATGAGACCTCGTATCGCTGTGG |
17az | tetX_gaR | 43 | 62.0 | CGGACTGCAGCGGCCGCTACTAGTATCAAGCAGCAGGGCAGCG |
17ba | addA1_gaF | 55 | 59.0 | TTTGCCGGACTGCAGCGGCCGCTACTAGTATTATTTGCCGACTACCTTGGTGATC |
17bb | addA1_gaR | 49 | 60.0 | TACAATGCTAGCGACAAAGAGGAGAAAGTAATGAGGGAAGCGGTGATCG |
17bc | addA1_gaF | 49 | 59.0 | TACAATGCTAGCGACAAAGAGGAGAAAGTAATGAGGGAAGCGGTGATCG |
17bd | addA1_gaR | 53 | 58.0 | TTTGCCGGACTGCAGCGGCCGCTACTAGTATTATTTGCCGACTACCTTGGTGA |
17be | aad9_gaF | 52 | 57.0 | GCTAGCGACAAAGAGGAGAAAGTAATGAGGAGGATATATTTGAATACATACG |
17bf | aad9_gaR | 73 | 54.0 | CGGACTGCAGCGGCCGCTACTAGTATTATAATTTTTTTAATCTGTTATTTAAATAGTTTATAGTTAAATTTAC |
17bg | aac3_gaF | 53 | 61.8 | TACAATGCTAGCGACAAAGAGGAGAAAGTAGTGCAATACGAATGGCGAAAAGC |
17bh | aac3_gaR | 50 | 62.6 | TTTGCCGGACTGCAGCGGCCGCTACTAGTATCAGCCAATCGACTGGCGAG |
17bi | tetA_gaF | 55 | 59.0 | TACAATGCTAGCGACAAAGAGGAGAAAGTAATGAAATCTAACAATGCGCTCATCG |
17bj | tetA_gaR | 48 | 60.0 | TTTGCCGGACTGCAGCGGCCGCTACTAGTATTAGGTCGAGGTGGCCCG |
17bk | pbp5_gaF | 57 | 59.0 | TACAATGCTAGCGACAAAGAGGAGAAAGTAATGGAACGAAGCAATCGTAATAAAAAG |
17bl | pbp5_gaR | 65 | 59.0 | TTTGCCGGACTGCAGCGGCCGCTACTAGTATTATTTAATGGTTGCTTCTAAGTAATCAATTAATG |
17bm | hyg_gaF | 61 | 66.7 | TACAATGCTAGCGACAAAGAGGAGAAAGTAGTGACACAAGAATCCCTGTTACTTCTCGACC |
17bn | hyg_gaR | 46 | 67.9 | TTTGCCGGACTGCAGCGGCCGCTACTAGTATCAGGCGCCGGGGGCG |
17bo | arpA_gaR | 51 | 61.0 | TTTGCCGGACTGCAGCGGCCGCTACTAGTATCAGCCTTTCGGTGTTTCCAC |
17bp | arpA_gaF | 50 | 61.0 | TACAATGCTAGCGACAAAGAGGAGAAAGTAATGCAATTCAAGCCAGCCGT |
17bq | ecoRI(1)35 | 35 | 63.0 | GAAGGTCGCCTGGAGTTCTCCGAAGTCGCGGTGGA |
17br | ecoRI(1)re | 35 | 63.0 | GCGACTTCGGAGAACTCCAGGCGACCTTCCTGCTT |
17bs | ecoRI(2)fw | 36 | 64.0 | CATTCTGATCGTGGAGTTCGCCAAGGAGCTGCACGA |
17bt | ecoRI(2)re | 40 | 64.0 | AGCTCCTTGGCGAACTCCACGATCAGAATGGCGTTTTTCG |
17bu | PlacUV5_ga1F | 55 | 56.0 | TTTACACTTTATGCTTCCGGCTCGTATAATGGACAAAGAGGAGAAAGTAATGGGC |
17bv | PlacUV5_ga1R | 19 | 58.0 | CAGCGGCCGCTACTAGTAC |
17bw | PlacUV5_ga2F | 19 | 58.0 | GTACTAGTAGCGGCCGCTG |
17bx | PlacUV5_ga2R | 48 | 58.0 | CATTATACGAGCCGGAAGCATAAAGTGTAAACTCTAGAAGCGGCCGCG |
17by | PtNTT2_gaF | 47 | 60.0 | GCTCGTATAATGGACAAAGAGGAGAAAGTAATGCGCCCGTATCCGAC |
17bz | PtNTT2_gaR | 58 | 57.0 | TTTGCCGGACTGCAGCGGCCGCTACTAGTATTATTTTTCTTTGTTGGTTTTCGCTTCC |
17ca | LFS_ovexPCR_F | 51 | 58.0 | TCTGACCAGGACTTTGAAAGCGTTTATGCGCATTGCCAGAGTGAAAATGCC |
17cb | LFS_ovexPCR_R | 50 | 57.0 | ACTGAGCCTTTCGTTTTATTTGATGCCTGGAATGCCTCCCGGATAAAACA |
17cc | Term_ovexPCR_F | 53 | 57.0 | TTTTCAGATGTGTTTTATCCGGGAGGCATTCCAGGCATCAAATAAAACGAAAG |
17cd | Term_ovexPCR_R | 50 | 57.0 | GAGCGCTCACAATTCTCACGCTTTCCAGTCTATAAACGCAGAAAGGCCCA |
17ce | lacO_ovexPCR_F | 41 | 58.0 | TGGGCCTTTCTGCGTTTATAGACTGGAAAGCGTGAGAATTG |
17cf | lacO_ovexPCR_R | 57 | 57.0 | ATCTAAGCCTATTGAGTATTTCTTATCCATCTAGTATTCCTGTGTGAAATTGTTATC |
17cg | cas9_ovexPCR_F | 58 | 57.0 | GCGGATAACAATTTCACACAGGAATACTAGATGGATAAGAAATACTCAATAGGCTTAG |
17ch | cas9_ovexPCR_R | 80 | 57.0 | ATGGTAATGTTGCTCATATCAGTATCTCATTTTACGGCGGTCGCCTTGACCGCCGACACCCTCAGTCACCTCCTAGCTGA |
17ci | RFS_ovexPCR_F | 80 | 57.0 | GAGTCAGCTAGGAGGTGACTGAGGGTGTCGGCGGTCAAGGCGACCGCCGTAAAATGAGATACTGATATGAGCAACATTAC |
17cj | RFS_ovexPCR_R | 59 | 58.0 | TTAGAACAGGAATGGATTTTCTTCTACACACAATAAATGTAATGCGTTATATCTTCCTG |
17ck | Sequ_ftsz_R | 23 | 53.0 | GTAAGTTCCATTGGTTCAAACAT |
17cl | Sequ_ftsz_F | 21 | 55.0 | CTGCGTAAGCAAGCTGATTAA |
17cm | Sequ_tet_R | 21 | 53.0 | GAGCGCATTGTTAGATTTCAT |
17cn | Sequ_tet_F | 20 | 65.0 | GCCGGGCCACCTCGACCTAA |
17co | Sequ_kan_R | 21 | 50.0 | CAATCCATCTTGTTCAATCAT |
17cp | Sequ_kan_F | 21 | 53.0 | CTTCTTGACGAGTTCTTCTGA |
17cq | codA_flanke1_delF | 46 | 59.0 | TGAGTTAGCTCACTCATTAGGCACCCATTAAAAGCGGTCGTTCCCG |
17cr | codA_flanke1_delR | 27 | 60.0 | TTATTCGACACTGTTAGCCTCCACATG |
17cs | codA_flanke2_delF | 44 | 60.0 | TGTGGAGGCTAACAGTGTCGAATAAACGACTGGGTTACAGCGAG |
17ct | codA_flanke2_delR | 45 | 58.0 | GACTGGCTTTCTACGTGTTCCGCTTCGGAATTTGCCACCAGAGAG |
17cu | sgRNA_LKA01_for | 24 | 0.0 | ACGCTATTGTTCATAATAGAAGAG |
17cv | sgRNA_LKA01_rev | 24 | 0.0 | AAACCTCTTCTATTATGAACAATA |
17cw | PtNTT2_assembly_gaF | 20 | 60.0 | GCGGCGGATAACGAACAGAG |
17cx | PtNTT2_assembly_gaR | 23 | 58.0 | TAATGCTCTGTTCGTTATCCGCC |
17cy | Sequ_MCS_F | 20 | 54.0 | GCGAATTGGGTACACTTACC |
17cz | Sequ_MCS_R | 21 | 54.0 | CACTAAAGGGAACAAAAGCTG |
17da | Sequ_pBK1_F | 20 | 64.0 | CACCTGACGCGCCCTGTAGC |
17db | Sequ_pBK2_F | 24 | 54.0 | GCATTCATTTTATGTTTCAGGTTC |
17dc | Sequ_pBK3_F | 21 | 54.0 | CAGCTTTTGTTCCCTTTAGTG |
17dd | Sequ_pBK4_F | 21 | 54.0 | CAGAAGAACTCGTCAAGAAGG |
17de | Sequ_54_F | 22 | 59.0 | GAACGCTCTCCTGAGTAGGACA |
17df | Sequ_55_F | 21 | 66.0 | GGCGCAGCGGTCGGACTGAAC |
17dg | Sequ_56_F | 21 | 64.0 | CTGCCCTGCTGAACTACCGCC |
17dh | Sequ_58_R | 21 | 60.0 | GTAATCGCTGAATTCCCGGCG |
17di | Prefix_fwd | 19 | 59.0 | TTTCTGGAATTCGCGGCCG |
17dj | Suffix_rev | 17 | 60.0 | TTTGCCGGACTGCAGCG |
17dk | KanMod-Fwd-1 | 57 | 61.0 | CATAAACAGTAATACAAGGGGTGTTATGTAGAGCCATATTCAACGGGAAACGTCTTG |
17dl | KanMod-Fwd-2 | 60 | 61.0 | CATAAACAGTAATACAAGGGGTGTTATGTAGTAGAGCCATATTCAACGGGAAACGTCTTG |
17dm | KanMod-Fwd-3 | 63 | 60.0 | CATAAACAGTAATACAAGGGGTGTTATGTAGTAGTAGAGCCATATTCAACGGGAAACGTCTTG |
17dn | KanMod-Rev | 32 | 61.0 | AACACCCCTTGTATTACTGTTTATGTAAGCAG |
17do | KanMod-Split-Fwd | 33 | 58.0 | GTAGTGATCTTATTTCATTATGGTGAAAGTTGG |
17dp | KanMod-Split-Rev | 33 | 58.0 | CCAACTTTCACCATAATGAAATAAGATCACTAC |
17dq | KanMod-19ML | 24 | 55.0 | GCTTAAACTCCAACTTGGACGCTG |
17dr | LacO_GA_For | 52 | 61.0 | ATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAG |
17ds | LacO_GA_Rev | 49 | 61.0 | GGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAG |
17dt | VR | 20 | 56.0 | GCTCACTCAAAGGCGGTAAT |
17du | VF2 | 20 | 57.0 | TGCCACCTGACGTCTAAGAA |
17dv | pK18-wo-ori_fwd | 14 | 59.0 | ACGCCAGCAACGCG |
17dw | pK18-wo-ori_rev | 29 | 60.0 | GAAGATCCTTTGATCTTTTCTACGGGGTC |
17dx | pSC101-replicon-gaF | 45 | 56.0 | CCGTAGAAAAGATCAAAGGATCTTCGAGTTATACACAGGGCTGGG |
17dy | pSC101-replicon_gaR | 49 | 57.0 | CGTAAAAAGGCCGCGTTGCTGGCGTTCAGATCCTTCCGTATTTAGCCAG |
17dz | RubiscoTAG2_fw | 50 | 68.0 | ATGCAGGATTACAAGCAAAGCCTCAAATATGAGACTTTCTCTTATCTTCC |
17ea | RubiscoTAG2_rv | 60 | 68.0 | TGAGGCTTTGCTTGTAATCCTGCATCTAAGCCATGATATCTTTCTCCTCTTTCTCTAGAA |
17eb | RubiscoTAG111_fw | 52 | 68.0 | AAAGAGGAGAAATACTAGATGGCAGTTAAGAAATATAGCGCGGGTGTCAAAG |
17ec | RubiscoTAG111_rev | 57 | 68.0 | CTGCCATCTAGTATTTCTCCTCTTTGATATCTTACTAGTTGCCGCGGTAGACCACGA |
17ed | RubisoTAG474_fw | 45 | 68.0 | TACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAGGGCAAGGTGTC |
17ee | RubisoTAG474_rev | 60 | 68.0 | CGGACTGCAGCGGCCGCTACTAGTAGATATCTTACTAACGGTTTTGTGTGTCCAACTTAT |
17ef | RubisomRFP_fw | 42 | 64.0 | CTTCGTGGTCTACCGCGGCAACTACTAGAGAATGGCTTCCTC |
17eg | RubiscomRFP_rev | 56 | 63.0 | GCGCTATATTTCTTAACTGCCATCTAGTATTTCTCCTCTTTGCGATCTACACTAGC |
17eh | mRFPRubisco_fw | 36 | 54.0 | GATAGTGCTAGTGTAGATCGCAAAGAGGAGAAATAC |
17ei | mRFPRubisco_rev | 33 | 55.0 | GTCTTCGGAGGAAGCCATTCTCTAGTAGTTGCC |
17ej | Sequ_pBAD_fw | 18 | 62.0 | GTTGCGTCGCGGTGCATG |
17ek | Sequ-pBAD_rev | 20 | 58.0 | CAATCTCACCATTCGCGGTC |
17el | VR | 20 | 56.0 | ATTACCGCCTTTGAGTGAGC |
17em | LFS_GA_F | 64 | 65.0 | AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGCATTGCCAGAGTGAAAATGCCTCAGAGCT |
17en | LFS_GA_R | 74 | 65.0 | CCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTAAATGCCTCCCGGATAAAACACATCTGAAAATTCATATGT |
17eo | BB_LFS_F | 56 | 69.0 | TTTTCAGATGTGTTTTATCCGGGAGGCATTTACTAGTAGCGGCCGCTGCAGTCCGG |
17ep | BB_LFS_R | 64 | 68.0 | TAGCTCTGAGGCATTTTCACTCTGGCAATGCTCTAGAAGCGGCCGCGAATTCCAGAAATCATCC |
17eq | Term_GA_F | 57 | 68.0 | ATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGCCAGG |
17er | Term_GA_R | 67 | 69.0 | GGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGG |
17es | BB_Term_F | 56 | 69.0 | CACCTTCGGGTGGGCCTTTCTGCGTTTATATACTAGTAGCGGCCGCTGCAGTCCGG |
17et | BB_Term_R | 64 | 68.0 | ACTGAGCCTTTCGTTTTATTTGATGCCTGGCTCTAGAAGCGGCCGCGAATTCCAGAAATCATCC |
17eu | lacO_GA_F2 | 62 | 68.0 | ATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGGACTGGAAAG |
17ev | lacO_GA_R2 | 67 | 69.0 | GGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATTCCTGTGTGAAATTG |
17ew | BB_lacO_F | 56 | 69.0 | TTGTGAGCGGATAACAATTTCACACAGGAATACTAGTAGCGGCCGCTGCAGTCCGG |
17ex | BB_lacO_R | 64 | 68.0 | GAGCGCTCACAATTCTCACGCTTTCCAGTCCTCTAGAAGCGGCCGCGAATTCCAGAAATCATCC |
17ey | Cas9_GA_F | 69 | 58.0 | AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGATGGATAAGAAATACTCAATAGGCTTAGATATCG |
17ez | Cas9_GA_R | 80 | 58.0 | GCCGGACTGCAGCGGCCGCTACTAGTATTTTACGGCGGTCGCCTTGACCGCCGACACCCTCAGTCACCTCCTAGCTGACT |
17fa | BB_Cas9_F | 80 | 69.0 | GAGTCAGCTAGGAGGTGACTGAGGGTGTCGGCGGTCAAGGCGACCGCCGTAAAATACTAGTAGCGGCCGCTGCAGTCCGG |
17fb | BB_Cas9_R | 64 | 68.0 | ATCTAAGCCTATTGAGTATTTCTTATCCATCTCTAGAAGCGGCCGCGAATTCCAGAAATCATCC |
17fc | RFS_GA_F | 74 | 62.0 | AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTGAGATACTGATATGAGCAACATTACCATTTATCACAAC |
17fd | RFS_GA_R | 80 | 62.0 | TTTGCCGGACTGCAGCGGCCGCTACTAGTACAATAAATGTAATGCGTTATATCTTCCTGATATAATTACCTTTGATTTCC |
17fe | BB_RFS_F | 56 | 69.0 | TCAGGAAGATATAACGCATTACATTTATTGTACTAGTAGCGGCCGCTGCAGTCCGG |
17ff | BB_RFS_R | 64 | 68.0 | AATGGTAATGTTGCTCATATCAGTATCTCACTCTAGAAGCGGCCGCGAATTCCAGAAATCATCC |
17fg | PtNTT2_trunc_gaF | 45 | 60.0 | TCGTATAATGGACAAAGAGGAGAAAGTAATGGCGGCAGCACCGTG |
17fh | aaRS_pC3_fw | 37 | 60.0 | CGGCCGCTTCTAGAGGCACCTGAAGTCAGCCCCATAC |
17fi | aaRS_RuTAG_rev | 40 | 60.0 | TATCTTTCTCCTCTTTGTGCAAGATTCCGAATACCGCAAG |
17fj | RuTAG_aaRS_fw | 38 | 54.0 | TTCGGAATCTTGCACAAAGAGGAGAAAGATATCATGGC |
17fk | RuTAG474_pC3_rv | 40 | 54.0 | CGGCCGCTACTAGTAGATATCTTACTAACGGTTTTGTGTG |
17fl | pC3_RuTAG474_fw | 45 | 70.0 | CGTTAGTAAGATATCTACTAGTAGCGGCCGCTGCAGTCCGGCAAA |
17fm | pC3_aaRS_rev | 55 | 70.0 | GCTGACTTCAGGTGCCTCTAGAAGCGGCCGCGAATTCCAGAAATCATCCTTAGCG |
17fn | pC3_RuTAG_fw | 45 | 70.0 | CACACAAAACCGTTAATACTAGTAGCGGCCGCTGCAGTCCGGCAA |
17fo | Sequ_pBad3 | 20 | 54.0 | GCACCACCCGGATGCCTATC |
17fp | Sequ_pBad4 | 21 | 54.0 | GTCCGCACCGCTGCCGGTAGC |
17fq | RuTAG_pC3_rv | 37 | 54.0 | TTGGACACACAAAACCGTTAATACTAGTAGCGGCCGC |
17fr | pSB1C3_splitF | 20 | 59.0 | GTCTGACAGCTCGAGGCTTG |
17fs | pSB1C3_splitR | 20 | 59.0 | CAAGCCTCGAGCTGTCAGAC |
17ft | PlacUV5_ga2R_v2 | 56 | 54.0 | TCTTTGTCCATTATACGAGCCGGAAGCATAAAGTGTAAACTCTAGAAGCGGCCGCG |
17fu | PlacUV5_ga2F_v2 | 18 | 57.0 | TACTAGTAGCGGCCGCTG |
17fv | pRedET_SeqF_1 | 20 | 57.0 | AGGCGTAGCAGAAACTAACG |
17fw | pRedET_SeqF_2 | 20 | 56.0 | CACACAAAGGTCTAGCGGAA |
17fx | pRedET_SeqR_1 | 18 | 59.0 | GACGCAGTGACGGCAATG |
17fy | pRedET_SeqR_2 | 20 | 58.0 | ATTTCAGCGACCTGTTTGGG |
17fz | pK18msb_ga1 | 20 | 58.0 | AAGCGGAACACGTAGAAAGC |
17ga | pK18msb_ga2 | 22 | 57.0 | GGTGCCTAATGAGTGAGCTAAC |
17gb | K1172904fw_gb1 | 51 | 58.0 | GATTTCTGGAATTCGCGGCCGCTTCTAGAGATGGCAGCACAGGTTATCAAC |
17gc | K1172904re_gb2 | 60 | 57.0 | TTTGCCGGACTGCAGCGGCCGCTACTAGTATTATCTGATTTTTGTAAAGGTCTGATAATG |
17gd | psB1A3fw_gb3 | 51 | 64.0 | CATTATCAGACCTTTACAAAAATCAGATAATACTAGTAGCGGCCGCTGCAG |
17ge | psB1A3re_gb4 | 51 | 63.0 | GTCAAACGTGTTGATAACCTGTGCTGCCATCTCTAGAAGCGGCCGCGAATT |
17gf | Glu107_fw | 63 | 67.0 | CTGAAAGCGAAATACGTCTATGGCAGCNNKGAACAGCTGGACAAAGACTACACCCTGAACGTG |
17gg | Glu107_rev | 64 | 67.0 | GCTGCCATAGACGTATTTCGCTTTCAGGCCCATCGCTTCGAACACTTTCTTGTTGTAGTCGCCG |
17gh | Asp158_Ile159_Leu162_fw | 73 | 67.0 | GAAGTGATCTATCCGATCATGCAGGTGAACNNKNNKCACTATNNKGGCGTAGATGTAGCAGTTGGTGGCATGG |
17gi | Asp158_Ile159_Leu162_rev | 59 | 67.0 | GTTCACCTGCATGATCGGATAGATCACTTCCGCCACTTTCGGGTTTTCATCTTCGCGGG |
17gj | pSB1C3_lin_fwd | 19 | 59.0 | TACTAGTAGCGGCCGCTGC |
17gk | GFPBB_fwd | 51 | 58.0 | CTGGAATTCGCGGCCGCTTCTAGAGATGCGTAAAGGAGAAGAACTTTTCAC |
17gl | pSB1C3_lin_rev | 17 | 61.0 | CTCTAGAAGCGGCCGCG |
17gm | GFPLink1_rev | 64 | 58.0 | CCCACCACTGCCTCCACTACCTCCAGAGCCCCCTTTGTATAGTTCATCCATGCCATGTGTAATC |
17gn | GFPLink2_rev | 64 | 58.0 | CCCACCACTGCCTCCCTAACCTCCAGAGCCCCCTTTGTATAGTTCATCCATGCCATGTGTAATC |
17go | Link1Step_fwd | 54 | 61.0 | GGGGGCTCTGGAGGTAGTGGAGGCAGTGGTGGGGCTGAAGCTGGTATCACCGGC |
17gp | Link2Step_fwd | 54 | 61.0 | GGGGGCTCTGGAGGTTAGGGAGGCAGTGGTGGGGCTGAAGCTGGTATCACCGGC |
17gq | StrepBB_rev | 45 | 61.0 | CTGCAGCGGCCGCTACTAGTATTAGGAAGCAGCGGACGGTTTAAC |
17gr | sup35_Histag_fw | 66 | 70.0 | CATCATCACCATCACCACCATCATCACCATTAATAATACTAGTAGCGGCCGCTGCAGTCCGGCAAA |
17gs | sup35_Histag_rev | 67 | 69.0 | GATGGTGGTGATGGTGATGATGAACTTCGTCGTCAACTTCTTCTTCCTGTTCTTTGATCAGAGCGTC |
17gt | pSB1C3_sup35_fw | 51 | 70.0 | GAAGTTGACGACGAAGTTTAATACTAGTAGCGGCCGCTGCAGTCCGGCAAA |
17gu | pSB1C3_sup35_rv | 56 | 70.0 | CCTGGTTAGAGTCAGACATCTCTAGAAGCGGCCGCGAATTCCAGAAATCATCCTTA |
17gv | T7RNAP_fw | 59 | 64.0 | GATTTCTGGAATTCGCGGCCGCTTCTAGAGATGAACACGATTAACATCGCTAAGAACGA |
17gw | T7RNAP_re | 50 | 64.0 | TTTGCCGGACTGCAGCGGCCGCTACTAGTATTACGCGAACGCGAAGTCCG |
17gx | psB1C3_T7P_fw | 50 | 63.0 | ATCTTAGAGTCGGACTTCGCGTTCGCGTAATACTAGTAGCGGCCGCTGCA |
17gy | psB1C3_T7P_re | 50 | 63.0 | GTCGTTCTTAGCGATGTTAATCGTGTTCATCTCTAGAAGCGGCCGCGAAT |
17gz | PtNTT2_seq_1 | 20 | 59.0 | GGCAACCAGGAAGTGACCAA |
17ha | PtNTT2_seq_2 | 20 | 60.0 | CACATCAATCGCCGCTTTGC |
17hb | PromRBS-Lin-fwd | 25 | 65.0 | TACTAGTAGCGGCCGCTGCAGTCCG |
17hc | KanR-Prä-fwd | 54 | 61.0 | ATGCTAGCTACTAGAGTCACACAGGAAAGATGAGCCATATTCAACGGGAAACGT |
17hd | PromRBS-Lin-rev | 23 | 57.0 | CTTTCCTGTGTGACTCTAGTAGC |
17he | KanMod-19ML-fwd | 43 | 65.0 | TCAGCATCCAAGTTGGAATTTAATCGCGGCCTGGAGCAAGACG |
17hf | KanMod-19ML-rev | 43 | 53.0 | GCGATTAAATTCCAACTTGGATGCTGATTTATATGGGTATAAA |
17hg | Suff-KanR-rev | 52 | 59.0 | TGCAGCGGCCGCTACTAGTAATTAGAAAAACTCATCGAGCATCAAATGAAAC |
17hh | neuKan-mod-TAG1 | 49 | 59.0 | ACTAGAGTCACACAGGAAAGATGTAGAGCCATATTCAACGGGAAACGTC |
17hi | neuKan-mod-TAG2 | 52 | 59.0 | ACTAGAGTCACACAGGAAAGATGTAGTAGAGCCATATTCAACGGGAAACGTC |
17hj | neuKan-mod-TAG3 | 55 | 59.0 | ACTAGAGTCACACAGGAAAGATGTAGTAGTAGAGCCATATTCAACGGGAAACGTC |
17hk | ONBY-out-rev | 26 | 54.0 | ATGAATCTCCAAGTATATAACGACTG |
17hl | ONBY-out-fwd | 27 | 63.0 | TAAGATCTGGATCCTCTACGCCGGACG |
17hm | NPA/ONBY-switch-fwd | 46 | 59.0 | TTATATACTTGGAGATTCATATGGACGAGTTTGAGATGATTAAGCG |
17hn | ONBY/NPA-switch-rev | 47 | 64.0 | TCCGGCGTAGAGGATCCAGATCTTACAGGCGTTTGCGGATTGGTTCC |
17ho | pK18msb_seq_1 | 20 | 55.0 | TAGCTATCGCCATGTAAGCC |
17hp | pK18msb_seq_2 | 20 | 62.0 | AGCGGAAGAGCGCCCAATAC |
17hq | aaRS_pSB1C3_fw | 50 | 58.0 | CGGCCGCTTCTAGAGGATTATCAATTTTAAAAAACTAACAGTTGTCAGCC |
17hr | aaRS_pSB1C3_rev | 39 | 58.0 | GCAGCGGCCGCTACTAGTACTCGAGCATGCAAAAAAGCC |
17hs | pSB1C3_aaRS_fw | 44 | 65.0 | GCTTTTTTGCATGCTCGAGTACTAGTAGCGGCCGCTGCAGTCCG |
17ht | pSB1C3_aaRS_rev | 50 | 65.0 | GTTTTTTAAAATTGATAATCCTCTAGAAGCGGCCGCGAATTCCAGAAATC |
17hu | sgRNA_romA_1_fw | 40 | 68.0 | CGTCAGTCCAGATACCTTTGATGTCCTGATTGGAAGGACC |
17hv | sgRNA_romA_1_rv | 40 | 68.0 | ACTCCTCCTCTCAAGGACTATAGAAAAAAAGTCCCGCTGC |
17hw | sgRNA_romG_2_fw | 40 | 68.0 | GCAGCGGGACTTTTTTTCTATAGTCCTTGAGAGGAGGAGT |
17hx | sgRNA_romG_2_rv | 40 | 69.0 | GCTCACAGGACACCTGGTATACGTATTGATCTCACGAGGC |
17hy | sgRNA_romd_3_fw | 40 | 69.0 | GCCTCGTGAGATCAATACGTATACCAGGTGTCCTGTGAGC |
17hz | sgRNA_romd_3_rv | 40 | 67.0 | GATTATCGGGCAAATACCTTCTCATACGTGACGGATTCCC |
17ia | sgRNA_romT_4_fw | 40 | 67.0 | GGGAATCCGTCACGTATGAGAAGGTATTTGCCCGATAATC |
17ib | sgRNA_romT_4_rv | 40 | 69.0 | ATACGTTCCCTTTAATTGCCTGCCGGAGTTAAGGACGTTC |
17ic | sgRNA_romC_5_fw | 40 | 69.0 | GAACGTCCTTAACTCCGGCAGGCAATTAAAGGGAACGTAT |
17id | sgRNA_romC_5_rv | 40 | 68.0 | GCTAGTATTACTCTGATCTGATGTTTGCAGTCGCTGCTGG |
17ie | Prefix_rev | 17 | 59.0 | CTCTAGAAGCGGCCGCG |
17if | pSC101-ori-part_gaF | 45 | 57.0 | CTGGAATTCGCGGCCGCTTCTAGAGGAGTTATACACAGGGCTGGG |
17ig | pSC101-ori-part_gaR | 49 | 58.0 | CGGACTGCAGCGGCCGCTACTAGTATCAGATCCTTCCGTATTTAGCCAG |
17ih | sgRNAs_Rom_gaF | 66 | 62.0 | GGCCTTTCTGCGTTTATATACTAGAAGCGGCCGCTGCACGTCAGTCCAGATACCTTTGATGTCCTG |
17ii | sgRNAs_Rom_gaR | 78 | 63.0 | AACTAACTAACCCTTAGTGACTCCTGCAGCGGCCGCTACTAGTAGCTAGTATTACTCTGATCTGATGTTTGCAGTCGC |
17ij | sgRNA_Rom_BB_gF | 32 | 62.0 | GCTGCAGGAGTCACTAAGGGTTAGTTAGTTAG |
17ik | sgRNA_Rom_BB_gR | 32 | 63.0 | CCGCTTCTAGTATATAAACGCAGAAAGGCCCA |
17il | SeqCas1 | 20 | 55.0 | ATTTGGCCTTAGCGCATATG |
17im | SeqCas2 | 20 | 58.0 | GGCTCCCCTATCAGCTTCAA |
17in | SeqCas3 | 21 | 56.0 | TCGATAAAGGTGCTTCAGCTC |
17io | SeqCas4 | 20 | 59.0 | GGTTGGGGACGTTTGTCTCG |
17ip | SeqCas5 | 22 | 56.0 | GGAAGAGACATGTATGTGGACC |
17iq | SeqCas6 | 20 | 60.0 | GCCGTCGTTGGAACTGCTTT |
17ir | SeqCas7 | 21 | 56.0 | CAGTCCTAGTGGTTGCTAAGG |
17is | SeqCas8 | 20 | 61.0 | AATCTTGGAGCTCCCGCTGC |
17it | Seq_LFS | 20 | 57.0 | CGTCCTGATTCAGACCTCCT |
17iu | Seq_B0015 | 20 | 58.0 | GGCTCAGTCGAAAGACTGGG |
17iv | RuBisCo_RFP_fw | 56 | 64.0 | GCTAGTGTAGATCGCAAAGAGGAGAAATACTAGATGGCAGTTAAGAAATATAGCGC |
17iw | Rubisco_RFP_rev | 37 | 64.0 | AGCCATTCTCTAGTAGTTGCCGCGGTAGACCACGAAG |
17ix | RFP_RuBisCo_rev | 49 | 64.0 | GTATTTCTCCTCTTTGCGATCTACACTAGCACTATCAGCGTTATTAAGC |
17iy | RFP_RuBisCo_fw | 43 | 64.0 | GGCAACTACTAGAGAATGGCTTCCTCCGAAGACGTTATCAAAG |
17iz | ori_pSB1C3_fw_66_3 | 39 | 66.0 | CCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTG |
17ja | pSB1C3_ori_rev_66_2 | 33 | 66.0 | CGGAGCCTATGGAAAAACGCCAGCAACGCGGCC |
17jb | pSB1C3_ori_fw_65_4 | 54 | 65.0 | CAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGC |
17jc | ori_pSB1C3_rev_65_3 | 57 | 65.0 | GTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGC |
17jd | J23100_gaF | 43 | 65.0 | CTTAGTGACTCGAATTCGCGGCCGCTTCTAGAGTTGACGGCTA |
17je | J23100_gaR | 56 | 65.0 | TAACTAACTAACCCTTAGTGACTCCTGCAGCGGCCGCTACTAGTATATAAACGCAG |
17jf | sgRNA_BB_gaF | 56 | 65.0 | CTGCAAACATCAGATCAGAGTAATACTAGCTACTAGTAGCGGCCGCTGCAGGAGTC |
17jg | sgRNA_BB_gaR | 62 | 65.0 | ATCAGGACATCAAAGGTATCTGGACTGACGTATAAACGCAGAAAGGCCCACCCGAAGGTGAG |
17jh | retBB_Rom_gaR | 38 | 61.0 | CTTACATTGTGTGGCTAGCACTGTACCTAGGACTGAGC |
17ji | retBB_Rom_gaF | 44 | 60.0 | ATCACGGTACTAGTGAAAGAGGAGAAATACTAGATGGCTTCCTC |
17jj | pSB1C3_aaRS(oT)_fw_66 | 55 | 65.0 | ATTTTAGAGCCAATTAGAAAGAGATTATAATACTAGTAGCGGCCGCTGCAGTCCG |
17jk | aaRS(oT)_pSB1C3_rev_58 | 71 | 58.0 | CCGGACTGCAGCGGCCGCTACTAGTATTATAATCTCTTTCTAATTGGCTCTAAAATCTTTATAAGTTCTTC |
17jl | ori_pSB1C3_fw_65 | 56 | 65.0 | TAAAAAGGCCGCGTTGCTGGCGTTGTGACCAAACAGGAAAAAACCGCCCTTAACAT |
17jm | neu_pSB1C3_ori_fw_58 | 51 | 58.0 | TACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGG |
17jn | pSB1C3_ori_rev_65 | 40 | 65.0 | GCGGTTTTTTCCTGTTTGGTCACAACGCCAGCAACGCGGC |
17jo | KanR-mod-fwd (80) | 80 | 72.0 | ACTAGAGTCACACAGGAAAGATGATTGAACAAGATGGACTACACGCAGGTTCTCCGGCCGCTTGGGTTGAGAGGCTATTC |
17jp | KanR-mod-rev (80) | 80 | 72.0 | CTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATCAGAAGAACTCGTCAAGAAGGCGATAGAAGGCGATGCGC |
17jq | KanR-TAG1-fwd (73) | 73 | 71.0 | AATGCTAGCTACTAGAGTCACACAGGAAAGATGTAGATTGAACAAGATGGACTACACGCAGGTTCTCCGGCCG |
17jr | KanR-TAG2-fwd (76) | 76 | 71.0 | AATGCTAGCTACTAGAGTCACACAGGAAAGATGTAGTAGATTGAACAAGATGGACTACACGCAGGTTCTCCGGCCG |
17js | KanR-TAG3-fwd (79) | 79 | 71.0 | AATGCTAGCTACTAGAGTCACACAGGAAAGATGTAGTAGTAGATTGAACAAGATGGACTACACGCAGGTTCTCCGGCCG |
17jt | KanR-mod-fwd-short (59) | 59 | 71.0 | ACTAGAGTCACACAGGAAAGATGATTGAACAAGATGGACTACACGCAGGTTCTCCGGCC |
17ju | pSB1C3_sup_fw | 70 | 70.0 | GATCAAAGAACAGGAAGAAGAAGTTGACGACGAAGTTTAATACTAGTAGCGGCCGCTGCAGTCCGGCAAA |
17jv | pSB1C3_sup_rev | 68 | 68.0 | GCTGGTTGTTACCCTGGTTAGAGTCAGACATCTCTAGAAGCGGCCGCGAATTCCAGAAATCATCCTTA |
17jw | sup35_121CUA_fw | 50 | 61.0 | CCAGGCTGGTTTCCAGCCGCAGCTACAGGGTATGTCTCTGAACGACTTCC |
17jx | sup35_121CUA_rv | 55 | 61.0 | TAGCTGCGGCTGGAAACCAGCCTGGTAACCTTGCAGGTTGTTGTTGTAGTTGAAG |
17jy | AcF_CUA_fw | 54 | 67.0 | CGGACTTAGAATCCGCATGGCAGGGGTTCAAATCCCCTCCGCCGGACCAAATTC |
17jz | AcF_CUA_rev | 50 | 67.0 | CCCTGCCATGCGGATTCTAAGTCCGCCGTTCTGCCCTGCTGAACTACCGC |
17ka | AB_BB_gaF | 33 | 69.0 | TACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAA |
17kb | AB_BB_gaR | 48 | 69.0 | TACTTTCTCCTCTTTGTCGCTAGCATTGTACCTAGGACTGAGCTAGCC |
17kc | tRNA_heid_fw | 53 | 61.0 | GATTTCTGGAATTCGCGGCCGCTTCTAGAGTGTGCTTCTCAAATGCCTGAGGC |
17kd | tRNA_heid_rev | 55 | 61.0 | TTTGCCGGACTGCAGCGGCCGCTACTAGTACATGCAAAAAAGCCTGCTCGTTGAG |
17ke | BB1C3_tRNA_h_fw | 51 | 62.0 | GCCTGCTCAACGAGCAGGCTTTTTTGCATGTACTAGTAGCGGCCGCTGCAG |
17kf | BB1C3_tRNA_h_re | 52 | 62.0 | CAAACTGGCCTCAGGCATTTGAGAAGCACACTCTAGAAGCGGCCGCGAATTC |
17kg | pSC101-pK18msb_seqF | 20 | 58.0 | GTTCCACTGAGCGTCAGACC |
17kh | pSC101-pK18msb_seqR | 20 | 56.0 | AACGCAGGAAAGAACATGTG |
17ki | TyrRS_pSB1C3_fw | 61 | 63.0 | GATTTTAGAGCCAATTAGAAAGAGATTATAACTGCAGTTTCAAACGCTAAATTGCCTGATG |
17kj | TyrRS_pSB1C3_rev | 63 | 63.0 | GTTATACGTTGTTTACGCTTTGAGGAATCCCATATGGACGAATTTGAAATGATAAAGAGAAAC |
17kk | PtNTT2_gaF_ext | 62 | 60.0 | CACTTTATGCTTCCGGCTCGTATAATGGACAAAGAGGAGAAAGTAATGCGCCCGTATCCGAC |
17kl | PtNTT2_gaR_ext | 68 | 56.0 | CTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATTATTTTTCTTTGTTGGTTTTCGCTTCC |
17km | LFS_Col | 27 | 58.0 | AATTTTCAGATGTGTTTTATCCGGGAG |
17kn | RFS_Col | 30 | 58.0 | GTGATAAATGGTAATGTTGCTCATATCAGT |
17ko | PtNTT2_trunc_gaF_v2 | 46 | 60.0 | TCGTATAATGGACAAAGAGGAGAAAGTAATGGGCGGCAGCACCGTG |
17kp | pSC101ori_ext_gaF | 43 | 64.0 | CCGTAGAAAAGATCAAAGGATCTTCAACGCCCTGAGAAGCCCG |
17kq | pSC101ori_ext_gaR | 47 | 63.0 | CGTAAAAAGGCCGCGTTGCTGGCGTGCATCGCCTGTTTCAGGCTGTC |
17kr | K80+RBS+T7RNAPre | 52 | 53.0 | CGTCTACATTACTAGTTTCTCCTCTTTGTTAGCCCAAAAAACGGGTATGGAG |
17ks | K80T7RNAPp1 | 61 | 53.0 | CCATACCCGTTTTTTGGGCTAACAAAGAGGAGAAACTAGTAATGTAGACGATTAACATCGC |
17kt | K808000+suff | 51 | 63.0 | CACCTTCGGGTGGGCCTTTCTGCGTTTATATACTAGTAGCGGCCGCTGCAG |
17ku | K808000+suf_re | 59 | 63.0 | CCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCG |
17kv | K808000+Term | 54 | 58.0 | ATCTTAGAGTCGGACTTCGCGTTCGCGTAACCAGGCATCAAATAAAACGAAAGG |
17kw | K808000+Term_re | 50 | 58.0 | GACTGAGCCTTTCGTTTTATTTGATGCCTGGTTACGCGAACGCGAAGTCC |
17kx | Pre+K808000 | 54 | 56.0 | GATTTCTGGAATTCGCGGCCGCTTCTAGAGTTATGACAACTTGACGGCTACATC |
17ky | Pre+K808000_re | 50 | 56.0 | TAGCCGTCAAGTTGTCATAACTCTAGAAGCGGCCGCGAATTCCAGAAATC |
17kz | T7RNAP_pos107_re | 20 | 58.0 | TTGCAGGAACTGGAAGGCTG |
17la | T7RNAP_pos107_BL | 22 | 58.0 | TTCCTGCAATAGATCAAGCCGG |
17lc | I746909+suff_rev | 67 | 60.0 | TTGCCGGACTGCAGCGGCCGCTACTAGTATAATACGACTCACTATAGGGAGATACTAGAGAAAGAGG |
17ld | Pre+I746909re | 52 | 58.0 | GATTTCTGGAATTCGCGGCCGCTTCTAGAGTATAAACGCAGAAAGGCCCACC |
17le | pre+I746909re_rev | 55 | 58.0 | GTGGGCCTTTCTGCGTTTATACTCTAGAAGCGGCCGCGAATTCCAGAAATCATCC |
17lf | Seq_RFS | 20 | 66.0 | CGCCTGTGCCGCCCTTCAGA |
17lg | pTarget_seq_fwd | 20 | 54.0 | GCCTTTGAGTGAGCTGATAC |
17lh | pTarget_seq_rev | 20 | 55.0 | CGGCACTGTTGCAAATAGTC |
17li | I746909+suff | 49 | 60.0 | TCTAGTATCTCCCTATAGTGAGTCGTATTATACTAGTAGCGGCCGCTGC |
17lj | PtNTT2_seq_fwd | 23 | 58.0 | CCAGGAAGTGACCAAATTTCTGC |
17lk | PtNTT2_seq_rev | 19 | 60.0 | GCAGGCTCTGAATGGTGCC |
17ll | pSC101-pK18msb-seq3 | 20 | 59.0 | GAGGAGCAAACAGCGTTTGC |
17lm | pSC101-pK18msb-seq4 | 25 | 59.0 | ATCTGTGCATATGGACAGTTTTCCC |
17ln | sup35_pSB1C3_fw | 69 | 65.0 | GATGATTTCTGGAATTCGCGGCCGCTTCTAGAGATGTCTGACTCTAACCAGGGTAACAACCAGCAGAAC |
17lo | sup35_pSB1C3_rv | 53 | 65.0 | GCCGCTACTAGTATTAAACTTCGTCGTCAACTTCTTCTTCCTGTTCTTTGATC |
17lp | TyrFS_pSB1C3_fw | 53 | 61.0 | CTTTGAGGAATCCCATATGGACGAATTTGAAATGATAAAGAGAAACACATCTG |
17lq | TyrFS_pSB1C3_rv | 67 | 61.0 | GCGTTTGAAACTGCAGTTATAATCTCTTTCTAATTGGCTCTAAAATCTTTATAAGTTCTTCAGCTAC |
17lr | sgRNA_BB_A_1_gF | 53 | 67.0 | TTCTATAGTCCTTGAGAGGAGGAGTTACTAGTAGCGGCCGCTGCAGGAGTCAC |
17ls | sgRNA_BB_G_2_gF | 58 | 67.0 | ATCAATACGTATACCAGGTGTCCTGTGAGCTACTAGTAGCGGCCGCTGCAGGAGTCAC |
17lt | sgRNA_BB_d_3_gF | 58 | 67.0 | CACGTATGAGAAGGTATTTGCCCGATAATCTACTAGTAGCGGCCGCTGCAGGAGTCAC |
17lu | sgRNA_BB_T_4_gF | 58 | 67.0 | AACTCCGGCAGGCAATTAAAGGGAACGTATTACTAGTAGCGGCCGCTGCAGGAGTCAC |
17lv | RomT_mutA_sense | 80 | nan | TAGCTCAGTCCTAGGTACAGTGCTAGCCACACAATGTAAGAATCACGGTACTAGTGAAAGAGGAGAAATACTAGATGGCT |
17lw | RomT_mutA_anti | 80 | nan | AGCCATCTAGTATTTCTCCTCTTTCACTAGTACCGTGATTCTTACATTGTGTGGCTAGCACTGTACCTAGGACTGAGCTA |
17lx | RomT_mutG_sense | 80 | nan | TAGCTCAGTCCTAGGTACAGTGCTAGCCACACAATGTAAGGATCACGGTACTAGTGAAAGAGGAGAAATACTAGATGGCT |
17ly | RomT_mutG_anti | 80 | nan | AGCCATCTAGTATTTCTCCTCTTTCACTAGTACCGTGATCCTTACATTGTGTGGCTAGCACTGTACCTAGGACTGAGCTA |
17lz | RomT_mutD_sense | 80 | nan | TAGCTCAGTCCTAGGTACAGTGCTAGCCACACAATGTAAGATCACGGTACTAGTGAAAGAGGAGAAATACTAGATGGCTT |
17ma | RomT_mutD_anti | 80 | nan | AAGCCATCTAGTATTTCTCCTCTTTCACTAGTACCGTGATCTTACATTGTGTGGCTAGCACTGTACCTAGGACTGAGCTA |
17mb | RomT_mutT_sense | 80 | nan | TAGCTCAGTCCTAGGTACAGTGCTAGCCACACAATGTAAGTATCACGGTACTAGTGAAAGAGGAGAAATACTAGATGGCT |
17mc | RomT_mutT_anti | 80 | nan | AGCCATCTAGTATTTCTCCTCTTTCACTAGTACCGTGATACTTACATTGTGTGGCTAGCACTGTACCTAGGACTGAGCTA |
17md | RomT_mutC_sense | 80 | nan | TAGCTCAGTCCTAGGTACAGTGCTAGCCACACAATGTAAGCATCACGGTACTAGTGAAAGAGGAGAAATACTAGATGGCT |
17me | RomT_mutC_anti | 80 | nan | AGCCATCTAGTATTTCTCCTCTTTCACTAGTACCGTGATGCTTACATTGTGTGGCTAGCACTGTACCTAGGACTGAGCTA |
17mf | ownT_mutA_sense | 80 | nan | CTAGCTCAGTCCTAGGTACAGTGCTAGCGATGATAGGCGGACTCTTCGGTACTAGTGAAAGAGGAGAAATACTAGATGGC |
17mg | ownT_mutA_anti | 80 | nan | GCCATCTAGTATTTCTCCTCTTTCACTAGTACCGAAGAGTCCGCCTATCATCGCTAGCACTGTACCTAGGACTGAGCTAG |
17mh | ownT_mutG_sense | 80 | nan | CTAGCTCAGTCCTAGGTACAGTGCTAGCGATGATAGGCGGGCTCTTCGGTACTAGTGAAAGAGGAGAAATACTAGATGGC |
17mi | ownT_mutG_anti | 80 | nan | GCCATCTAGTATTTCTCCTCTTTCACTAGTACCGAAGAGCCCGCCTATCATCGCTAGCACTGTACCTAGGACTGAGCTAG |
17mj | ownT_mutT_sense | 80 | nan | CTAGCTCAGTCCTAGGTACAGTGCTAGCGATGATAGGCGGTCTCTTCGGTACTAGTGAAAGAGGAGAAATACTAGATGGC |
17mk | ownT_mutT_anti | 80 | nan | GCCATCTAGTATTTCTCCTCTTTCACTAGTACCGAAGAGACCGCCTATCATCGCTAGCACTGTACCTAGGACTGAGCTAG |
17ml | ownT_mutC_sense | 80 | nan | CTAGCTCAGTCCTAGGTACAGTGCTAGCGATGATAGGCGGCCTCTTCGGTACTAGTGAAAGAGGAGAAATACTAGATGGC |
17mm | ownT_mutC_anti | 80 | nan | GCCATCTAGTATTTCTCCTCTTTCACTAGTACCGAAGAGGCCGCCTATCATCGCTAGCACTGTACCTAGGACTGAGCTAG |
17mn | pSC101-replace-fwd | 45 | 71.0 | TATGAAATTGGTGGTTGATAAGCGAGGCCGCCCGACTGATACGTT |
17mo | pSC101-replace-rev | 55 | 64.0 | GCTTATCAACCACCAATTTCATATTGCTGTAAGTGTTTAAATCTTTACTTATTGG |
17mp | pK18msB_split_1 | 29 | 66.0 | GCAACTACAGCTCAGGCGACAACCATACG |
17mq | pK18msB_split_2 | 29 | 66.0 | CGTATGGTTGTCGCCTGAGCTGTAGTTGC |
17mr | sup_21TGT_fw | 50 | 62.0 | CCAGCAGTACTCTCAGAATGGATGTCAACAGCAAGGCAATAATCGTTATC |
17ms | sup_21TGT_rev | 70 | 63.0 | GTTGACATCCATTCTGAGAGTACTGCTGGTAATTCTGCTGGTTGTTACCCTGGTTAGAGTCAGACATCTC |
17mt | TyrRS_FluoAS_fw | 67 | 63.0 | GATTAAAATCCTGGAGCCCATTCGCAAGCGCCTGTAACTGCAGTTTCAAACGCTAAATTGCCTGATG |
17mu | TyrRS_FluoAS_rv | 70 | 63.0 | GATGTGTTACGCTTAATCATCTCGAACTCGTCCATATGGGATTCCTCAAAGCGTAAACAACGTATAACGG |
17mv | ONBY-out-rev_2 | 35 | 63.0 | ATGAATCTCCAAGTATATAACGACTGCACCTTTGC |
17mw | CLRS-fw | 40 | 73.0 | GAATTCGCGGCCGCTTCTAGAGTTTTAAAAAACTAACAGT |
17mx | CLRS-rv | 40 | 80.0 | CTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCC |
17my | PrK_210CTT_fw | 41 | 69.0 | CCGAAAGACGAAATCAGCCTTAATTCCGGCAAACCGTTTCG |
17mz | PrK_210CTT_rv | 39 | 69.0 | AAGGCTGATTTCGTCTTTCGGATTCAGCAGAACCTCCAG |
17na | PrK_456CTT_fw | 46 | 69.0 | CTTTAGTTTCAAACGCTAAATTGCCTGATGCGCTACGCTTATCAGG |
17nb | PrK_456CTT_rv | 54 | 69.0 | CAATTTAGCGTTTGAAACTAAAGTTACAGGTTCGTAGAGATCCCGTTATAATAC |
17nc | AcF_308CTT_fw | 46 | 69.0 | CTTCAGTTTCAAACGCTAAATTGCCTGATGCGCTACGCTTATCAGG |
17nd | AcF_308CTT_rv | 54 | 69.0 | CAATTTAGCGTTTGAAACTGAAGTTATAATCTCTTTCTAATTGGCTCTAAAATC |
17ne | AcF_107GAG_fw | 59 | 69.0 | AGTGAGTTCCAGCTTGATAAGGATTATACACTGAATGTCTATAGATTGGCTTTAAAAAC |
17nf | AcF_107GAG_rv | 56 | 69.0 | GTGTATAATCCTTATCAAGCTGGAACTCACTTCCATAAACATATTTTGCCTTTAAC |
17ng | sup35_121CTA_fw | 51 | 70.0 | CAAGCTGGATTCCAGCCTCAACTACAGGGGATGTCGCTTAATGACTTTCAG |
17nh | sup35_121CTA_rv | 49 | 70.0 | CCCCTGTAGTTGAGGCTGGAATCCAGCTTGATAACCTTGCAAATTGTTG |
17ni | sup35_Histag_rev | 73 | 70.0 | TTAATGGTGATGATGGTGGTGATGGTGATGATGAACTTCGTCGTCAACTTCTTCTTCCTGTTCTTTAATTAAC |
17nj | blocking_L_fw | 42 | 72.0 | CGGCCTGCTGACTTTCTCGCCGATCAAAAGGCATTTTGCTAT |
17nk | blocking_L_rv | 37 | 72.0 | GCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCC |
17nl | blocking_R_fw | 72 | 70.0 | GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAACTTGAAAAAGTGGCACCGAGTCG |
17nm | blocking_R_rv | 48 | 70.0 | AAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTAAAAAAAGCACCG |
17nn | sgRNA_biobrck_F | 44 | 63.0 | CTAGCATCTAGTGACTCTAGAGCTAAGCGGCCTGCTGACTTTCT |
17no | sgRNA_biobrck_R | 59 | 63.0 | GAACTAGACAGATTCTGCAGCGGCCGCTACTAGTAAAAAAAGCCTGCTCGTTGAGCAGG |
17np | FluoAS_308CTT | 67 | 69.0 | CATCAGGCAATTTAGCGTTTGAAACTGAAGTTACAGGCGCTTGCGAATGGGCTCCAGGATTTTAATC |
17nq | Prüf_ori_fw_58 | 32 | 58.0 | CCTTTTTCTTTAAAACCGAAAAGATTACTTCG |
17nr | Prüf_ori_rev_58 | 22 | 58.0 | CGGGGCGTAATTTGATATCGAG |
17ns | NPA_seq_fwd | 19 | 56.0 | CCATTTGGGTGATCTTGGC |
17nt | NPA_seq_rev | 24 | 55.0 | CAAGGATTCTAACTCCTCATACGA |
17nu | GFP_tag_gaF | 55 | 58.0 | GAACAAAAGCTTATTTCTGAAGAGGACTTGCGTAAAGGAGAAGAACTTTTCACTG |
17nv | GFP_tag_gaR | 57 | 54.0 | TTTGCCGGACTGCAGCGGCCGCTACTAGTATTATTTGTATAGTTCATCCATGCCATG |
17nw | PtNTT2_tag_gaR | 55 | 56.0 | CAAGTCCTCTTCAGAAATAAGCTTTTGTTCTTTTTCTTTGTTGGTTTTCGCTTCC |
17nx | KanR_fw | 54 | 55.0 | AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGATGAGCCATATTCAACGGG |
17ny | KanR_rev | 49 | 53.0 | GCCGGACTGCAGCGGCCGCTACTAGTATTAGAAAAACTCATCGAGCATC |
17nz | KanR_S133Am | 23 | 63.0 | GCGCCGGTTGCATTAGATTCCTG |
17oa | KanR_S133Am_rev | 19 | 63.0 | GCGCAGGAACACTGCCAGC |
17ob | KanR_S154Am | 26 | 63.0 | CAGGCGCAATAGCGAATGAATAACGG |
17oc | KanR_S154Am_rev | 23 | 65.0 | GCCTGAGCGAGACGAAATACGCG |
17od | pSB1C3_PF_rev | 52 | 60.0 | CTGGAGCAAGACGTTTCCCGTTGAATATGGCTCATCTCTAGAAGCGGCCGCG |
17oe | pSB1C3_SF_fw | 54 | 60.0 | TGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAATACTAGTAGCGGCCGCTGC |
17of | T7RNAP_BL_fw | 20 | 62.0 | CAGACATTGCCGTCACTGCG |
17og | T7RNAP_BL_rev | 17 | 61.0 | GCCACGAAGACCACGCC |
17oh | T7_CBS_fw | 63 | 63.0 | GACTCACTATAGGGAATACAAGCTACTTGTTCTTTTTGCATACTAGTAGCGGCCGCTGCAGTC |
17oi | T7_CBS_rev | 72 | 63.0 | CAAGTAGCTTGTATTCCCTATAGTGAGTCGTATTACTCTAGTATTAGCTATTCAGATTTGCGATACACCACG |
17oj | KanR_rev_fw | 56 | 52.0 | GGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTAGAAAAACTCATCGAGCATC |
17ok | KanR_rev_rev | 54 | 52.0 | CCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTAATGAGCCATATTCAACGG |
17ol | mutT_flanke1_gaF | 50 | 59.0 | TAATGTGAGTTAGCTCACTCATTAGGCACCCTCGATTTGCCAATTGCCGA |
17om | mutT_flanke1_gaR | 55 | 55.0 | GTCGGATGCGGCGAAAACGCCTTATCTGACTGTCTTAAACCTATAAAAAAGGCGC |
17on | mutT-flanke2_gaF | 50 | 56.0 | ATTCTGCGCCTTTTTTATAGGTTTAAGACAGTCAGATAAGGCGTTTTCGC |
17oo | mutT-flanke2-gaR | 49 | 54.0 | CTGCGGACTGGCTTTCTACGTGTTCCGCTTGGCACTTACCATGGTGCTG |
17op | Cas9only_F | 64 | 58.0 | GATTTCTGGAATTCGCGGCCGCTTCTAGAGATGGATAAGAAATACTCAATAGGCTTAGATATCG |
17oq | Cas9only_R | 51 | 58.0 | TTTGCCGGACTGCAGCGGCCGCTACTAGTATCAGTCACCTCCTAGCTGACT |
17or | bb_Cas9only_F | 55 | 67.0 | ATTGATTTGAGTCAGCTAGGAGGTGACTGATACTAGTAGCGGCCGCTGCAGTCCG |
17os | bb_Cas9only_R | 60 | 66.0 | ATCTAAGCCTATTGAGTATTTCTTATCCATCTCTAGAAGCGGCCGCGAATTCCAGAAATC |
17ot | crtI_86TAG_fw | 51 | 68.0 | GAACTGCTGCCGGTTACGCCGTAGTACCGCCTGTGTTGGGAGTCAGGGAAG |
17ou | crtI_86TAG_rev | 64 | 68.0 | ACGGCGTAACCGGCAGCAGTTCGACATACTCTTTTAACTGTTTTCCTGCCAGTGCAAACAGTTC |
17ov | crtI_318TAG_fw | 55 | 68.0 | CTATTTTGGTTTGAATCACCATCATTAGCAGCTCGCGCATCACACGGTTTGTTTC |
17ow | crtI_318TAG_rev | 61 | 68.0 | ATGATGGTGATTCAAACCAAAATAGAGCACAAACAGAGAGTTACTCATGCGCTTAGTTTGC |
17ox | crtI_353TAG_fw | 49 | 68.0 | GAGGACTTCTCACTTTATCTGTAGGCGCCCTGTGTCACGGATTCGTCAC |
17oy | crtI_353TAG_rev | 60 | 68.0 | CAGATAAAGTGAGAAGTCCTCTGCGAGGCCATCATGATTAAAAATTTCGTCAATCAGCTC |
17oz | BB_ovexGA_F | 18 | 52.0 | GATTTCTGGAATTCGCGG |
17pa | BB_ovexGA_R | 15 | 52.0 | TTTGCCGGACTGCAG |
17pb | B0015_ovex_F | 54 | 57.0 | ATTGATTTGAGTCAGCTAGGAGGTGACTGACCAGGCATCAAATAAAACGAAAGG |
17pc | B0015_ovex_R | 51 | 57.0 | AATGGTAATGTTGCTCATATCAGTATCTCATATAAACGCAGAAAGGCCCAC |
17pd | B0015_GA_F | 54 | 57.0 | GATTTCTGGAATTCGCGGCCGCTTCTAGAGCCAGGCATCAAATAAAACGAAAGG |
17pe | B0015_GA_R | 51 | 57.0 | TTTGCCGGACTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCAC |
17pf | Cas9o_ovex_F | 68 | 57.0 | TGTGAGCGGATAACAATTTCACACAGGAATACTAGATGGATAAGAAATACTCAATAGGCTTAGATATC |
17pg | Cas9o_ovex_R | 50 | 56.0 | ACTGAGCCTTTCGTTTTATTTGATGCCTGGGTCAGCTAGGAGGTGACTGA |
17ph | RFS_ovex_F | 57 | 56.0 | CACCTTCGGGTGGGCCTTTCTGCGTTTATATGAGATACTGATATGAGCAACATTACC |
17pi | RFS_GA57_R | 60 | 57.0 | TTTGCCGGACTGCAGCGGCCGCTACTAGTACAATAAATGTAATGCGTTATATCTTCCTGA |
17pj | Seq_crtE1_fw | 22 | 58.0 | CTACGAGGATCAGGGGTTTACC |
17pk | Seq_crtE2_fw | 22 | 58.0 | GTGGTTCATACCTATCGCGACC |
17pl | Cas9o_ovex_R2 | 50 | 56.0 | ACTGAGCCTTTCGTTTTATTTGATGCCTGGTCAGTCACCTCCTAGCTGAC |
17pm | 28567c3g1_gF | 64 | 59.0 | TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGCCTTGGATCCTAAAGTGAAG |
17pn | 28567c3g1_gR | 61 | 59.0 | CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGCATTCCCATTCGATGGTTGATGG |
17po | 27205c6g2i2_gF | 63 | 58.0 | TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGCTTTAGAATTGCAAGACGG |
17pp | 27205c6g2i2_gR | 67 | 58.0 | CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGCAGAAGGCCTTTTTCTCATAAGAAATGAG |
17pq | 24513c0g1_gF | 65 | 58.0 | TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGCTTCTTCAGCTTCCAATAATG |
17pr | 24513c0g1i3_gR | 73 | 58.0 | CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGCAAGAGAAAAGTCGTTTTTCATATGAGTATAAACC |
17ps | 24513c0g1i1_gR | 73 | 58.0 | CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGCAAGAATAGAGACGTTTTTCATATGAGTATAAACC |
17pt | 27205c6g2i4_gR | 63 | 58.0 | CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGCATGTAGGGAACATCCAGAAGCTTA |
17pu | 107595c0g1i1_gF | 64 | 59.0 | TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGAACCTCAATTCTCTCCGCCTC |
17pv | 107595c0g1i1_gR | 64 | 59.0 | CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGCACTTGTATATCAAGGCATCTCGTCC |
17pw | 30924c1g1i14_gF | 63 | 58.0 | TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGGGTCGTTGAAAAATGAAGG |
17px | 30924c1g1i14_gR | 64 | 58.0 | CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGCAAACACTAAGCTTGGGATGGTAATC |
17py | 30983c0g1i12_gF | 65 | 61.0 | TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGTGTTCCAAAAAATAGCAGCCC |
17pz | 30983c0g1i12_gR | 57 | 61.0 | CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGCACTTGCCGCCCGTTTTCC |
17qa | 26152c4g1i4_gF | 75 | 59.0 | TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGAAAACGAAAACAAAATAGAAAGTAACAGTAG |
17qb | 26152c4g1i4_gR | 68 | 59.0 | CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGCATTTACCATCCATTTTCCACTGTAAGTAC |
17qc | 29270c2g2i7_gF | 64 | 59.0 | TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGAAGGTCAATATCCACAGCAG |
17qd | 29270c2g2i7_gR | 67 | 59.0 | CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGCACACAAGCTGCACAACAGCAGCCACTCG |
17qe | 30924c1g1i5_gR | 60 | 58.0 | CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGCAAAGCAGTTCCATCCGTGTAG |
17qf | 26152c4g1i8_gF | 63 | 58.0 | TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGAGAATCAAACTGCTCTTGC |
17qg | L_rob_pSB1C3_fw_65 | 52 | 65.0 | CCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTGGTCCTGGCCACGGGTGC |
17qh | L_pSB1C3_rop_rev_65 | 49 | 65.0 | GCCGCGTTGCTGGCGTTGGTCCTGGCCACGGGTGCGCATGATCGTGCTC |
17qi | k_rop_pSB1C3_fw_66 | 53 | 65.0 | CCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTGCGGAAGTCAGCGCCCTGC |
17qj | k_pSB1C3_rop_rev_65 | 50 | 65.0 | CGGAACATAATGGTGCAGGGCGCTGACTTCCGCAACGCCAGCAACGCGGC |
17qk | Cas9_EcoRI_F | 55 | 67.0 | AGACAATCGTGAGAAGATTGAAAAAATCTTGACTTTTCGCATCCCTTATTATGTT |
17ql | Cas9_EcoRI_R | 50 | 73.0 | TTGCCACGCGCCAATGGACCAACATAATAAGGGATGCGAAAAGTCAAGAT |
17qm | 28626c0g1i1_gF | 65 | 59.0 | TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGAGCAGTACAGATTCGTAATCG |
17qn | 28626c0g1i1_gR | 65 | 58.0 | CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGCAACAGGACTTATTAGAACTGCAATGG |
17qo | CLRS-fw_OL | 50 | 77.0 | TTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTTTAAAA |
17qp | CLRS-rv_OL | 50 | 83.0 | CCTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATATAAACGC |
17qq | gRNA_fw_codA | 25 | 0.0 | TAGC CACAGTGAACGTCGATGAGA |
17qr | gRNA_rv_codA | 25 | 0.0 | AAAC TCTCATCGACGTTCACTGTG |
17qs | pSB3K5-UBP-gaF | 19 | 60.0 | GCCGCTGCAGGAGTCACTA |
17qt | pSB3K5-UBP-gaR | 22 | 59.0 | CCGCGAATTCGAGTCACTAAGG |
17qu | mRFP-UBP-gaF | 22 | 59.0 | CCCTTAGTGACTCGAATTCGCG |
17qv | mRFP-Link-ccdB-gaR | 43 | 63.0 | AAACCTTAAACTGTGCGGCTGCCGCAGCACCGGTGGAGTGACG |
17qw | ccdB-Linker-mRFP-gaF | 58 | 60.0 | CTCCACCGGTGCTGCGGCAGCCGCACAGTTTAAGGTTTACACCTATAAAAGAGAGAGC |
17qx | ccdB-UBP_gaR | 60 | 60.0 | TAGTGACTCCTGCAGCGGCCGCTACTAGTATTATATTCCCCAGAACATCAGGTTAATGGC |
17qy | ccdB-UBP_gaF | 63 | 59.0 | TAGTGAAAGAGGAGAAATACTAGATGGCTTCAGTTTAAGGTTTACACCTATAAAAGAGAGAGC |
17qz | mRFP-UBP_gaR | 47 | 62.0 | GTGGCTAGCACTGTACCTAGGACTGAGCTAGCACCGGTGGAGTGACG |
17ra | PtNTT2_bb_gaR | 32 | 59.0 | CATTACTTTCTCCTCTTTGTCCATTATACGAG |
17rb | PtNTT2-noSP_gaF | 52 | 61.0 | CTCGTATAATGGACAAAGAGGAGAAAGTAATGCTGCCGGTGAAAAAAGGCAC |
17rc | CLtRNA_fw | 47 | 72.0 | TTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTGTGC |
17rd | CLtRNA_rv | 30 | 72.0 | GCCGCTGCAGTCCGGCAAAAAAGGGCAAGG |
17re | RDL_SELP_fw | 50 | 72.0 | TTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGAGAGGAGG |
17rf | RDL_SELP_rv | 30 | 72.0 | CTTGCCCTTTTTTGCCGGACTGCAGCGGCC |
17rg | XbaI_RBS_baBL_fw | 51 | 60.0 | CTTCTAGAGAAAGAGGAGAAACTAGTAATGGCAGCATAGGTTATCAACACG |
17rh | SpeI_baBL_rev | 45 | 60.0 | GCTACTAGTATTATCTGATTTTTGTAAAGGTCTGATAATGGTCCG |
17ri | Rubisco_110ATT_fw | 46 | 57.0 | CGCGGCAATTAGTAAGATATCAAAGAGGAGAAATACTAGATGGCAG |
17rj | Rubisco_110ATT_rv | 41 | 57.0 | GATATCTTACTAATTGCCGCGGTAGACCACGAAGGCCAGAC |
17rk | BcuI_R | 40 | 68.0 | TCCTCTTTCACAAGAAGCTAGCACTGTACCTAGGACTGAG |
17rl | BcuI_F | 40 | 66.0 | TGCTAGCTTCTTGTGAAAGAGGAGAAATACTAGATGGCTT |
17rm | MHA_GA_F | 60 | 69.0 | ATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTGACGGC |
17rn | MHA_GA_R | 70 | 68.0 | CGTATAACGTTACTGGTTTCATCTAGTATTTCTCCTCTTTCACAAGAAGCTAGCACTGTACCTAGGACTG |
17ro | lacI_BB_R | 62 | 68.0 | TACCTAGGACTGAGCTAGCCGTCAACTCTAGAAGCGGCCGCGAATTCCAGAAATCATCCTTA |
17rp | lacI_BB_F | 67 | 69.0 | TCTTGTGAAAGAGGAGAAATACTAGATGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGT |
17rq | fwd_CDD_pSB1C3 | 56 | 61.0 | AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGATGCAAGCCAAACCATTTCAC |
17rr | rev_CDD_pSB1C3 | 71 | 62.0 | CCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATCAATTGAAATTTAAATCTAATGTATCAATTGAATC |
17rs | Split_RBS_F | 45 | 60.0 | TACTAGTGAAAGAGGAGAAATACTAGAGATGGCTTCCTCCGAAGA |
17rt | Split_RBS_R | 46 | 59.0 | TTTCTCCTCTTTCACTAGTATGTGTGAAATTGTTATCCGCTCACAA |
17ru | PtNTT2-pelB-SP_gaF | 65 | 59.0 | GCGGCGGCGGGCCTGCTGCTGCTGGCGGCGCAGCCGGCGATGGCGCTGCCGGTGAAAAAAGGCAC |
17rv | PtNTT2_bb_pelB-SP_gaR | 74 | 55.0 | CAGCAGCAGCAGGCCCGCCGCCGCGGTCGGCAGCAGATATTTCATTACTTTCTCCTCTTTGTCCATTATACGAG |
17rw | PtNTT2_TAT-SP_gaF | 77 | 56.0 | ACCACAGGACTTGCCACTATCGGCGCTGCCAGCATGTTTATGCCAAAGGCCAACGCCCTGCCGGTGAAAAAAGGCAC |
17rx | PtNTT2_bb_TAT-SP_gaR | 86 | 60.0 | GCCGATAGTGGCAAGTCCTGTGGTGGCTTTTAAGAAGCCTCGGCGGTTTATTTGCATTACTTTCTCCTCTTTGTCCATTATACGAG |
17ry | PtNTT2-only_gaF | 43 | 63.0 | CTGGAATTCGCGGCCGCTTCTAGAGATGCGCCCGTATCCGACC |
17rz | fwd_CDD_SpeI | 50 | 50.0 | TGATGAAAAATGTGGCTAATACCAGTGTTTTGAAATGGGGTGGAATATTG |
17sa | rev_CDD_SpeI | 76 | 76.0 | AATATTCCACCCCATTTCAAAACACTGGTATTAGCCACATTTTTCATCACCTTGGTATTTCCAAATTTCTTACCTC |
17sb | AcF_107GAG2_fw | 62 | 64.0 | GATAAGGATTATACACTGAATGTCTATAGATTGGCTTTAAAAACTACCTTAAAAAGAGCAAG |
17sc | AcF_107GAG2_rv | 48 | 64.0 | GACATTCAGTGTATAATCCTTATCAAGCTGGAACTCACTTCCATAAAC |
17sd | PtNTT2-lacO-gaR | 53 | 55.0 | TCGACTGAGCCTTTCGTTTTATTTGTTATTTTTCTTTGTTGGTTTTCGCTTCC |
17se | lacO-PtNTT2_gaF | 50 | 59.0 | AAGCGAAAACCAACAAAGAAAAATAACAAATAAAACGAAAGGCTCAGTCG |
17sf | lacO-PtNTT2_gaR | 54 | 57.0 | ATCTAAGCCTATTGAGTATTTCTTATCCATTTCCTGTGTGAAATTGTTATCCGC |
17sg | Cas9_gaF | 63 | 56.0 | TTGTGAGCGGATAACAATTTCACACAGGAAATGGATAAGAAATACTCAATAGGCTTAGATATC |
17sh | is_kan_fw | 44 | 61.0 | CCAATTAACCAATTCTGAATGATTGAACAAGATGGATTGCACGC |
17si | is_kan_rev | 53 | 61.0 | CATAAACAGTAATACAAGGGGTGTTTCAGAAGAACTCGTCAAGAAGGCGATAG |
17sj | bb_kan_fv | 56 | 61.0 | CGCCTTCTTGACGAGTTCTTCTGAAACACCCCTTGTATTACTGTTTATGTAAGCAG |
17sk | bb_kan_rv | 53 | 61.0 | GCAATCCATCTTGTTCAATCATTCAGAATTGGTTAATTGGTTGTAACACTGGC |
17sl | pSB_CFP_fw | 54 | 69.0 | GTCACTCCACCGGTGCTTAATACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAG |
17sm | pSB_YFP_rev | 60 | 69.0 | CAGTTCTTCGCCTTTGCTCATCTCTAGAAGCGGCCGCGAATTCCAGAAATCATCCTTAGC |
17sn | CFP_YFP_fw | 50 | 63.0 | GAATTCGCGGCCGCTTCTAGAGATGAGCAAAGGCGAAGAACTGTTTACCG |
17so | CFP_pSB_rev | 64 | 63.0 | GAACCAGCAGCGGAGCCAGCGGATCCCATTTTATACAGTTCATCCATGCCATGGGTAATACCGG |
17sp | YFP_CFP_fw | 67 | 63.0 | GGGATCCGCTGGCTCCGCTGCTGGTTCTGGCGAATAGATGGCTTCCTCCGAAGACGTTATCAAAGAG |
17sq | YFP_pSB_rev | 53 | 63.0 | CCGGACTGCAGCGGCCGCTACTAGTATTAAGCACCGGTGGAGTGACGACCTTC |
17sr | fwd_CCD_Topo | 25 | 60.0 | CCGTTTCTCATCGAATATGCAAGCC |
17ss | rev_CCD_Topo | 26 | 61.0 | GCCCAAAAACCATGAAATCCAAGTGG |
17st | fwd_pTXB1_backb | 21 | 65.0 | TGCATCACGGGAGATGCACTA |
17su | rev_pTXB1_backb | 45 | 66.0 | ATGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGG |
17sv | pC3_YFP_fw | 49 | 69.0 | GATGAACTGTATAAATACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAG |
17sw | pC3_CFP_rv | 51 | 69.0 | GGAGGAAGCCATCTCTAGAAGCGGCCGCGAATTCCAGAAATCATCCTTAGC |
17sx | YFP_pC3_fw | 60 | 63.0 | GATTTCTGGAATTCGCGGCCGCTTCTAGAGATGGCTTCCTCCGAAGACGTTATCAAAGAG |
17sy | YFP_CFP_rv | 52 | 63.0 | CAGAACCAGCAGCGGAGCCAGCGGATCCCATAGCACCGGTGGAGTGACGACC |
17sz | CFP_YFP_fw | 56 | 63.0 | TGGCTCCGCTGCTGGTTCTGGCGAATAGATGAGCAAAGGCGAAGAACTGTTTACCG |
17ta | CFP_pC3_rev | 58 | 63.0 | GGACTGCAGCGGCCGCTACTAGTATTTATACAGTTCATCCATGCCATGGGTAATACCG |
17tb | B0015_ba_fw | 58 | 63.0 | CCATTATCAGACCTTTACAAAAATCAGATAACCAGGCATCAAATAAAACGAAAGGCTC |
17tc | B0015_ba_r_rev | 64 | 63.0 | CGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTATAAACGCAGAAAGGCCCACCCG |
17td | baBL_fw | 67 | 60.0 | CTCCATACCCGTTTTTTGGGCTAACAAAGAGGAGAAACTACTAATGGCAGCATAGGTTATCAACACG |
17te | baBL_rev | 70 | 60.0 | CGACTGAGCCTTTCGTTTTATTTGATGCCTGGTTATCTGATTTTTGTAAAGGTCTGATAATGGTCCGTTG |
17tf | K80_ba_rev | 70 | 56.0 | GATAACCTATGCTGCCATTAGTAGTTTCTCCTCTTTGTTAGCCCAAAAAACGGGTATGGAGAAACAGTAG |
17tg | K808000_ba_r_rev | 60 | 56.0 | CCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATTATGACAACTTGACGGCTACATC |
17th | pSB1C3_ba_r_fw | 66 | 63.0 | GGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATACTAGTAGCGGCCGCTGCAG |
17ti | pSB1C3_ba_r_rev | 64 | 63.0 | GGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATACTCTAGAAGCGGCCGCGAATTCCAGAAATC |
17tj | pSB1C3_prom_fw | 69 | 65.0 | CCTCTTTGCTAGCACTGTACCTAGGACTGAGCTAGCCGTCAATACTAGTAGCGGCCGCTGCAGTCCGGC |
17tk | Term_kanR_rev | 69 | 54.0 | CCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTATAAACGCAGAAAGGCCC |
17tl | Term_kanR_fw | 53 | 54.0 | GCAGTTTCATTTGATGCTCGATGAGTTTTTCTAACCAGGCATCAAATAAAACG |
17tm | pSB1C3_term_rev | 70 | 65.0 | CTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATACTCTAGAAGCGGCCGCGAATTCCAGAAATCATCC |
17tn | kanR_term_rev | 71 | 55.0 | GGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGTTAGAAAAACTCATCGAGCATCAAATG |
17to | kanR_prom_fw | 70 | 55.0 | GACGGCTAGCTCAGTCCTAGGTACAGTGCTAGCAAAGAGGAGAAAGTAGTAATGAGCCATATTCAACGGG |
17tp | SpeI_weg_fw | 24 | 54.0 | GAGGAGAAAGTAGTAATGTAGACG |
17tq | SpeI_weg_rev | 21 | 54.0 | CTTTCTCCTCTTTGTTAGCCC |
17tr | qPCR_mRFP_F | 23 | 62.0 | AAGGTCGTCCGTACGAAGGTACC |
17ts | qPCR_mRFP_R | 25 | 63.0 | TCAGCCGGGTGTTTAACGTAAGCTT |
17tt | Cm_pSB6A1_fw_53 | 42 | 53.0 | GAGTAAACTTGGTCTGACAGGATTCTCACCAATAAAAAACGC |
17tu | Cm_pSB6A1_rev_53 | 46 | 53.0 | GTTAATGTCATGATAATAATGGTTTCTTAGACCGTCAGGTGGCACT |
17tv | pSB6A1_Cm_fw_52 | 53 | 52.0 | CCCGATCAACTCGAGTGCCACCTGACGGTCTAAGAAACCATTATTATCATGAC |
17tw | pSB6A1_Cm_rev_52 | 54 | 52.0 | GGTTGCCGCCGGGCGTTTTTTATTGGTGAGAATCCTGTCAGACCAAGTTTACTC |
17tx | mRFP-UBP-New_gaR | 47 | 62.0 | ATCGCTAGCACTGTACCTAGGACTGAGCTAGCACCGGTGGAGTGACG |
17ty | ccdB-UBP-Max_gaF | 64 | 58.0 | ACTAGTGAAAGAGGAGAAATACTAGATGGCTCAGTTTAAGGTTTACACCTATAAAAGAGAGAGC |
17tz | ccdB-UBP-New_gaF | 64 | 59.0 | ATAGTGAAAGAGCAGAAATACTAGATGGCTTCAGTTTAAGGTTTACACCTATAAAAGAGAGAGC |
17ua | codA_lin_fwd | 21 | 59.0 | CATTAAAAGCGGTCGTTCCCG |
17ub | codA-lin_rev | 20 | 59.0 | CGGAATTTGCCACCAGAGAG |
17uc | mRFP1_CBT-Asp_fw | 35 | 70.0 | TTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCT |
17ud | mRFP1_CBT-Asp_rv | 26 | 70.0 | CCTTTTTTGCCGGACTGCAGCGGCCG |
17ue | Cas9_Split_F | 32 | 61.0 | ATCTTGACTTTTCGCATCCCTTATTATGTTGG |
17uf | Cas9_Split_R | 32 | 61.0 | CCAACATAATAAGGGATGCGAAAAGTCAAGAT |
17ug | RBS_tight_F | 43 | nan | TTTCACACAGGAATACTAGATGGCTTCCTCCGAAGACGTTATC |
17uh | RBS_tight_R | 41 | nan | CATCTAGTATTCCTGTGTGAAATTGTTATCCGCTCACAATT |
17ui | lacUV5_split_F | 35 | nan | TATGCTTCCGGCTCGTATAATGTGTGAAATTGTGA |
17uj | lacUV5_split_R | 35 | nan | TCACAATTTCACACATTATACGAGCCGGAAGCATA |
17uk | RBS_B0034_F | 49 | nan | ACAATTAAAGAGGAGAAATACTAGATGGCTTCCTCCGAAGACGTTATCA |
17ul | RBS_B0034_R | 47 | nan | CATCTAGTATTTCTCCTCTTTAATTGTTATCCGCTCACAATTTCACA |
17um | RBS_B0035_F | 52 | nan | AATTATTAAAGAGGAGAATACTAGATGGCTTCCTCCGAAGACGTTATCAAAG |
17un | RBS_B0035_R | 49 | nan | CATCTAGTATTCTCCTCTTTAATAATTGTTATCCGCTCACAATTTCACA |
17uo | kan_alt_bb_1 | 23 | 64.0 | TACTAGTAGCGGCCGCTGCAGTC |
17up | kan_alt_bb_2 | 18 | 63.0 | CCCTTCGGGAAGCGTGGC |
17uq | kan_alt_bb_3 | 21 | 63.0 | GGTCTGACAGCTCGAGTCCCG |
17ur | kan_alt_bb_4 | 24 | 61.0 | CCGTTATTCATTCGTGATTGCGCC |
17us | kan_alt_bb_5 | 32 | 60.0 | CACCCCTTGTATTACTGTTTATGTAAGCAGAC |
17ut | pos_kanRr_fw | 53 | 60.0 | AGCCTGCTCAACGAGCAGGCTTTTTTGCATGTATAAACGCAGAAAGGCCCACC |
17uu | pos_B0015_rev | 63 | 60.0 | CACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATATATAAACGCAGAAAGGCCCACCC |
17uv | pos_kanRr_rev | 55 | 60.0 | AAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGACGGCTAGCTCAGTCCTAGG |
17uw | pos_T7RNAP_fw | 69 | 60.0 | CTTTGCTAGCACTGTACCTAGGACTGAGCTAGCCGTCAATTATGACAACTTGACGGCTACATCATTCAC |
17ux | pos_tRNA_rev | 55 | 60.0 | CACCTTCGGGTGGGCCTTTCTGCGTTTATACATGCAAAAAAGCCTGCTCGTTGAG |
17uy | pSC_mRFP_fw | 55 | 68.0 | GTGCTAGTGTAGATCGCCTCTAGAAGCGGCCGCGAATTCCAGAAATCATCCTTAG |
17uz | pSC_mRFP_rev | 67 | 68.0 | CTCCTCTTTCTCTAGTATCTCCCTTTAGTGAGTGTTAATTTACTAGTAGCGGCCGCTGCAGTCCGGC |
17va | mRFP_pSC_fw | 64 | 63.0 | GGGAGATACTAGAGAAAGAGGAGAAATACTAGAGATGGCTTCCTCCGAAGACGTTATCAAAGAG |
17vb | mRFP_pSC_rev | 49 | 63.0 | CGGCCGCTTCTAGAGGCGATCTACACTAGCACTATCAGCGTTATTAAGC |
17vc | T3Pol_2TAG_fw | 55 | 63.0 | ATGTAGTAGATCATCGAAAACATCGAAAAGAATGACTTCTCAGAAATCGAACTGG |
17vd | T3Pol_2TAG_rev | 67 | 63.0 | CGATGTTTTCGATGATCTACTACATCTAGTACTTTCCTGTGTGACTCTAGTATTAGAAAAACTCATC |
17ve | Bb-pRS_RFP_fw_61 | 24 | 61.0 | GTTGATGTTGCTGTTGGAGGGATG |
17vf | Bb-pRS_RFP_rev_61 | 37 | 61.0 | CAACCTTTGGATTTTCATCCTCTCTTGCTATAAGTTC |
17vg | mRFP_RS_fw_60 | 58 | 60.0 | GAACTTATAGCAAGAGAGGATGAAAATCCAAAGGTTGCAATACGCAAACCGCCTCTCC |
17vh | mRFP_RS_rev_60 | 50 | 60.0 | CTCCATCCCTCCAACAGCAACATCAACTATAAACGCAGAAAGGCCCACCC |
17vi | 158_159_162 _fw 55 | 73 | 55.0 | GAGTATGGAACTTATAGCAAGAGAGGATGAAAATCCAAAGGTTGCTGAAGTTATCTATCCAATAATGATGGTT |
17vj | 158-159-162 rev 55 | 77 | 55.0 | CTCCATCCCTCCAACAGCAACATCAACGCCMNNATAATGCAGMNNMNNAACCATCATTATTGGATAGATAACTTCAG |
17vk | S133_S155_fw_59 | 74 | 59.0 | GCCTGAGCGAGACGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCTAATGCAACCGGCGCAG |
17vl | S155_S133_rev_59 | 61 | 59.0 | GCGATCGCGTATTTCGTCTCGCTCAGGCGCAATAGCGAATGAATAACGGTTTGGTTGATGC |
17vm | Prüf_S155_S133_rev_59 | 18 | 59.0 | CTGCGCCGGTTGCATTAG |
17vn | T7RNAP_spe_fw_59 | 63 | 59.0 | CCGTTTTTTGGGCTAACAAAGAGGAGAAAGTAGTAATGTAGACGATTAACATCGCTAAGAACG |
17vo | T7RBS_Spe_rev_61 | 62 | 61.0 | CGTTCTTAGCGATGTTAATCGTCTACATTACTACTTTCTCCTCTTTGTTAGCCCAAAAAACG |
17vp | T7RNAP_107_fw_59 | 48 | 59.0 | GCCCGACAGCCTTCCAGTTCCTGCAATAGATCAAGCCGGAAGCCGTAG |
17vq | T7RNAP_107_rev_59 | 52 | 59.0 | GTGATGTACGCTACGGCTTCCGGCTTGATCTATTGCAGGAACTGGAAGGCTG |
17vr | Prüf_T7RNAP_107_rev_59 | 19 | 59.0 | CGGCTTCCGGCTTGATCTA |
17vs | gBlock_delta_own2_s | 18 | 56.0 | GTGAGCGATGCTCCGTTG |
17vt | UBP_PCR_1C3_fw | 21 | 56.0 | CCTTAGCTTTCGCTAAGGATG |
17vu | UBP_PCR_1C3_rv | 19 | 56.0 | CGAATTGCAAGACGCTGTC |
17vv | Prefix-AG_rev | 20 | nan | CTAGAAGCGGCCGCGAATTC |
17vw | PtNTT2-AG_gaF | 43 | nan | TTCTGGAATTCGCGGCCGCTTCTAGATGCGCCCGTATCCGACC |
17vx | codA-flank-part_gaF | 46 | nan | CTGGAATTCGCGGCCGCTTCTAGAGCATTAAAAGCGGTCGTTCCCG |
17vy | codA-flank-part_gaR | 45 | nan | CGGACTGCAGCGGCCGCTACTAGTACGGAATTTGCCACCAGAGAG |
17vz | mRFP_sacB_gaR | 55 | nan | CTTGTTTTGCAAACTTTTTGATGTTTGCGGCTGCCGCAGCACCGGTGGAGTGACG |
17wa | sacB_ccdB-rep_gaF | 57 | nan | CGTCACTCCACCGGTGCTGCGGCAGCCGCAAACATCAAAAAGTTTGCAAAACAAGCA |
17wb | sacB-ccdB-rep_gaR | 63 | nan | TAGTGACTCCTGCAGCGGCCGCTACTAGTATTATTTGTTAACTGTTAATTGTCCTTGTTCAAG |
17wc | alt_VR_pSB1C3 | 21 | 57.0 | CCTGCATAACGCGAAGTAATC |
17wd | sacB-UBP-Max_gaF | 60 | nan | GTACTAGTGAAAGAGGAGAAATACTAGATGGCTAACATCAAAAAGTTTGCAAAACAAGCA |
17we | sacB-UBP-New_gaF | 60 | nan | GTATAGTGAAAGAGCAGAAATACTAGATGGCTTAACATCAAAAAGTTTGCAAAACAAGCA |
17wf | retBB_sgRNA_gF | 64 | 65.0 | CGAAAAGCCTGCTCAACGAGCAGGCTTTTTTACTAGAGTTGACGGCTAGCTCAGTCCTAGGTAC |
17wg | retBB_sgRNA_gR | 58 | 66.0 | GCTTAGTTGCATCTAGGTGACGCATCTCTCTCTCTAGAAGCGGCCGCGAATTCGAGTC |
17wh | mRFP-retBB_o2_f | 37 | 56.0 | CTTCGGTACTAGTGAAAGAGGAGAAATACTAGATGGC |
17wi | mRFP-retBB_o2_r | 31 | 57.0 | CCTATCATCGCTAGCACTGTACCTAGGACTG |
17wj | Split_pSB3C5_for | 26 | 59.0 | TGTATCACATATTCTGCTGACGCACC |
17wk | Split_pSB3C5_rev | 26 | 59.0 | GGTGCGTCAGCAGAATATGTGATACA |
17wl | Split_SpeI_for | 40 | 61.0 | TGCTAGCTGCTTGTGAAAGAGGAGAAATACTAGATGGCTT |
17wm | Split_SpeI_rev | 40 | 61.0 | TCCTCTTTCACAAGCAGCTAGCACTGTACCTAGGACTGAG |
17wn | lacUV5_Split_F | 65 | 55.0 | TATGCTTCCGGCTCGTATAATGGACAAAGAGGAGAAAGTAATGGATAAGAAATACTCAATAGGCT |
17wo | lacUV5_Split_R | 60 | 53.0 | CTCTTTGTCCATTATACGAGCCGGAAGCATAAAGTGTAAATATAAACGCAGAAAGGCCCA |
17wp | pSB1K3_Split_F | 24 | 59.0 | GTAAACTTGGTCTGACAGCTCGAG |
17wq | pSB1K3_Split_R | 24 | 59.0 | CTCGAGCTGTCAGACCAAGTTTAC |
17wr | lacNEU_F | 80 | 61.0 | TCGGCTCGTATAATGTGTGAAATTGTGAGCGGATAACAATTTCACACAAGGAGATTACTAGATGGATAAGAAATACTCAA |
17ws | lacNEU_R | 80 | 64.0 | GTGAAATTGTTATCCGCTCACAATTTCACACATTATACGAGCCGATGATTAATTGTCAACAGCCTGGGGTGCCTAAATTG |
17wt | lacNEU_BB_F | 46 | 67.0 | ACAATTTCACACAAGGAGATTACTAGTAGCGGCCGCTGCAGTCCGG |
17wu | lacNEU_BB_R | 53 | 69.0 | AATTCTCACGCTTTCCAGTCCTCTAGAAGCGGCCGCGAATTCCAGAAATCATC |
17wv | ColPCR_Mis_F | 22 | 55.0 | CCAGGCTGTTGACAATTAATCA |
17ww | ColPCR_R | 18 | 55.0 | CCTTCTACGAGCTGTCCG |
17wx | ColPCR2_F | 20 | 55.0 | CCGGGAGGCATTTACTAGAG |
17wy | c3_g1_i4_fw | 27 | 58.0 | CTTAAAGGTGTTGAACATGGTGAGATG |
17wz | c2_g1_i4_rv | 25 | 58.0 | ACCATGTTCAACACCTTTAAGCTTG |
17xa | c3g1i4_gBl_gF | 68 | 57.0 | TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGCATTAGATCCTAAGGTTAAAGATC |
17xb | c3g1i4_gBl_gR | 58 | 57.0 | CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGCTCCCACTCAATGGTCGAAG |
17xc | pSB3C5_split_fwd | 20 | 56.0 | GTTTCGCCACCACTGATTTG |
17xd | pSB3C5_split_rev | 20 | 60.0 | ACGCTCAAATCAGTGGTGGC |
17xe | pSB3C5-SpeI-remo-gaF | 50 | 63.0 | TACAGTGCTAGCTCATAGTGAAAGAGGAGAAATACTAGATGGCTTCCTCC |
17xf | pSB3C5-SpeI-remo_gaR | 45 | 60.0 | TAGTATTTCTCCTCTTTCACTATGAGCTAGCACTGTACCTAGGAC |
17xg | sacB-Test-gaF | 43 | nan | GCGGCAGCCGCAGCAGCCGCAACCCGCGATACCAAAGATACCC |
17xh | mRFP-Test-gaR | 41 | nan | TGCGGCTGCTGCGGCTGCCGCAGCACCGGTGGAGTGACGAC |
17xi | sacB-test-gaF | 47 | nan | CGTTCGGACTTCACACGGCCAACATCAAAAAGTTTGCAAAACAAGCA |
17xj | sacB-test-gaR | 46 | nan | GGCCGTGTGAAGTCCGAACGACCACGCTCACGCTGCTATAAATTTC |
17xk | pTXB1_GMPSopt_f | 59 | 59.0 | TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGCGCTGGATCCAAAAG |
17xl | pTXB1_GMPSopt_r | 61 | 59.0 | CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGTTCCCATTCGATTGTCGACGGAG |
17xm | pTXB1_CDAopt_f | 69 | 59.0 | TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGAACAATATCGCTTTGTAATTGAAGC |
17xn | pTXB1_CDAopt_r | 68 | 59.0 | CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGGCAGCTTTTGTTAGATGAACAATGAAACAC |
17xo | phyto_fw | 38 | 56.0 | AACGTCTCACTCGATCGATGCAAGCCAAACCATTTCAC |
17xp | phyto_rv | 53 | 54.0 | AACGTCTCACTCAATCGTCAATTGAAATTTAAATCTAATGTATCAATTGAATC |
17xq | GFP-NPtNTT2_gaF | 58 | nan | GCTCGTATAATGGACAAAGAGGAGAAAGTAATGCGTAAAGGAGAAGAACTTTTCACTG |
17xr | PtNTT2-NGFP_gaR | 49 | nan | AGTTCTTCTCCTTTACGCATTACTTTCTCCTCTTTGTCCATTATACGAG |
17xs | GFP-NPtNTT2_gaR | 64 | nan | GTCGGATACGGGCGTGCGGCTGCCGCTGCGGCTGCCGCTTTGTATAGTTCATCCATGCCATGTG |
17xt | PtNTT2-NGFP_gaF | 58 | nan | GATGAACTATACAAAGCGGCAGCCGCAGCGGCAGCCGCACGCCCGTATCCGACCATTG |
17xu | PtNTT2_seq2_fwd | 20 | nan | GGCCTGGTGTTTGTGTTTGG |
17xv | PtNTT2(31-575)-T7-gaF | 43 | nan | TAATACGACTCACTATAGGGAAAGAGGAGAAAGTAATGCTGCC |
17xw | PtNTT2-T7-gaF | 43 | nan | TAATACGACTCACTATAGGGAAAGAGGAGAAAGTAATGCGCCC |
7xx | PtNTT2-T7-gaR | 47 | nan | TCTCCTCTTTCCCTATAGTGAGTCGTATTACTCTAGAAGCGGCCGCG |
17xy | MHA-mRFP-seqR | 20 | nan | ACCTTCGTACGGACGACCTT |
17xz | CWH-mRFP_seqF | 20 | nan | CGGGTGCTTACAAAACCGAC |
17ya | CWH-sacB-seqR | 21 | nan | CGTGTAATATGGGAAATGCCG |
17yb | mRFPretBB_25_gF | 25 | 56.0 | GAAAGAGGAGAAATACTAGATGGCT |
17yc | mRFPretBB_25_gR | 21 | 57.0 | AGCACTGTACCTAGGACTGAG |
17yd | PtNTT2_histag_gaF | 48 | nan | CACCATCACCACCATCATCACCATTAATAATACTAGTAGCGGCCGCTG |
17ye | PtNTT2_histag_gaR | 55 | nan | ATGGTGATGATGGTGGTGATGGTGATGATGTTTTTCTTTGTTGGTTTTCGCTTCC |
17yf | Seq_Library_fw | 90 | 57.0 | AATGGGGTTAAAGGCAAAATATGTTTATGGAAGTGAATTCCAGCTTGATAAGGATTATACACTGAATGTCTATAGATTGGCTTTAAAAAC |
17yg | Seq_Library_rev | 81 | 58.0 | CTGCCCAGCTGGAGTTGTTGAAGGAAATCCAATAATGGAGATAGCTAAATACTTCCTTGAATATCCTTTAACCATAAAAAG |
17yh | ColPCR_PtNTT2_F | 21 | 55.0 | AAAATGGAAGCGAAAACCAAC |
17yi | split_kan_fw | 43 | nan | GTGCCCTGAATGAACTGCAAGACGAGGCAGCGCGGCTATCGTG |
17yj | split_kan_rv | 53 | nan | TCGTCTTGCAGTTCATTCAGGGCACCGGACAGGTCGGTCTTGACAAAAAGAAC |
17yk | split_kan_ggü_fw | 29 | nan | GGCGGTAATACGGTTATCCACAGAATCAG |
17yl | split_kan_ggü_rv | 29 | nan | CTGATTCTGTGGATAACCGTATTACCGCC |
17ym | Seq_57_fw_B8 | 89 | nan | AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTNCAGCTTGATAAGGATTATACACTGAATGTC |
17yn | Seq_58_fw_B11 | 92 | nan | AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTNGCTTGATAAGGATTATACACTGAATGTCTATAG |
17yo | Seq_57_fw_B21 | 89 | nan | AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTNGGATTATACACTGAATGTCTATAGATTGGC |
17yp | Seq_57_fwd_B3 | 90 | nan | AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTNCACTGAATGTCTATAGATTGGCTTTAAAAAC |
17yq | Seq_58_B8_rev | 81 | nan | CAAGCAGAAGACGGCATACGAGATGCCTAAGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCACAACTCCAGCTGGGCAG |
17yr | Seq_57_alt_rev | 87 | nan | CAAGCAGAAGACGGCATACGAGATGCCTAAGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCNGATTTCCTTCAACAACTCCAGCT |
17ys | Seq_57_B11_rev | 91 | nan | CAAGCAGAAGACGGCATACGAGATGTAGCCGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTCCATTATTGGATTTCCTTCAACAACTC |
17yt | Seq_56_B21_rev | 93 | nan | CAAGCAGAAGACGGCATACGAGATCGAAACGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCGGATATTCAAGGAAGTATTTAGCTATCTCC |