Team:Hong Kong UCCKE/Experiments
Assay
We have done assays on the cultured cells.
The aim of doing these assays was to test whether part 300,400 and 500 are valid. We expect there will be a positively proportional trend when putting part 300 into increasing concentrations of uric acid. The higher the concentration is, the more GFP expression. Below I explain the steps and calculations involved in these assays in detail.
We first incubate the cells for 24 hours and then pipette them into different concentrations of uric acid. We expect that there will be an increase in GFP expression when it was put in higher uric acid concentration. When there is no uric acid, or in very low concentration, the strong repressor KRAB-HucR will stop the expression and thus no GFP expression. For Column 1-3, 4-6, 7-9, and 10-12, they are uric acid with water, K2197300, K2197400, and K2197500 respectively.
General Steps:
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