Team:NTHU Taiwan/Results
NTHU_Taiwan
PART I Degradation
1. Cloning of Horseradish Peroxidase
To make sure the plasmid is cloned into E.coli BL-21 strain, we extracted the plasmid from transformed E.coli.(figure 1) We validated the gene by PCR with specific primers and then we examined the result with Agarose gel electrophoresis. A successful cloning was verified from the results of a PCR performed with designed specific primers according to the theoretically expected length of horseradish peroxidase(927bp).
