Team:NTHU Taiwan/Contribution
NTHU_Taiwan
1. interlab measurement study
We have finished the interlab experiments and get lots of results in the page of interlab
2. Contribution of new parts
We have contributed new 12 biobricks for iGEM part registry and submitted 9 parts to iGEM headquarter.(Figure 1)
Figure 1. the list of our new 12 parts
3. Validation of composite parts’ function
We constructed three composite parts and all of their function have been validated:
(1)Part:BBa_K2354010:monobody-GBP HS
Monobody was fused with gold binding protein and linked with 6×his tag. This fused protein can bind to the gold surface by the function of the gold-binding protein, and it and binds to ER-alpha/EDCs complex.
(2)Part:BBa_K2354011:INP-ER-alpha HS
ER-alpha was fused with ice nucleating protein. This fused protein can be anchored to the membrane of E.coli via the function of ice nucleating protein and ER-alpha can bind to EDCs for detection.
(3)Part:BBa_K2354012:HRP-his tag
Horseradish peroxidase is one kind of peroxidase which has heme as a cofactor, and it can degrade EDCs to the less toxic compounds. Moreover, we linked fused protein with 6×his tag for purification.
