Team:Freiburg/Achievements

Overview Motivation CAR T Cells Tumor Microenvironment AND Gate Outlook Achievements References

Members Attributions Partners Contact

Main Project Modeling Applied Design Proof of Concept BioBricks Basic Part BioBrick Improvement Interlab Study

Safety Cloning HIF1A Knockdown Cell Culture Lab Book

Human Practice Integrated Human Practice Collaborations

Achievements

Most important achievements

Knock Down

We designed...

Kill Switch

Text

Stable Cell lines

Stable cell lines for promoter characterization, knock-down of HIF1A and kill switch were generated via lentiviral transduction and sorted by FACS.

Requirements for a Gold Medal

Integrated Human Practice

The goal of CARTEL™ is to create a safer immune-based therapy. Hearing many concerns about the safety of our project and conducting a survey, we integrated further safety precautions. We successfully tested a construct containing a thymidine kinase which makes T cells sensitive to ganciclovir induced apoptosis. We went over a realistic embedment of our project in the context of acceptance and side effects. Additionally, we evaluated possibilities to optimize the manufacturing process of CAR T cells with the aim to contribute to a therapy, which is affordable and versatile in its use for many cancer patients. Finally, a survey was able to confirm the importance and the need for immunotherapies, which have potential to improve the efficacy and safety of cancer therapies.

Requirements for a Silver Medal

Requirements for a Bronze Medal