Team:NTHU Taiwan/Composite Part
NTHU_Taiwan
We constructed three composite parts and all of their function have been validated:
Monobody was fused with gold binding protein and linked with 6×his tag. This fused protein can bind to the gold surface by the function of gold binding protein, and monobody can bind to ER-alpha/EDCs complex.
BBa_K2354011
ER-alpha was fused with ice nucleating protein. This fused protein can be anchored on the membrane of E.coli via the function of ice nucleating protein and ER-alpha can bind to EDCs for detection.
BBa_K2354012
Horseradish peroxidase is one kind of peroxidase which has heme as cofactor, and it can degrade EDCs to the less toxic compounds. Moreover, we linked fused protein with 6×his tag for purification.
