Team:IISc-Bangalore/Assembly

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1. BioBrick Transformations
2. Plasmid Isolation
3. PCRs
4. Restriction digests
5. Ligations
6. Screening

BioBrick Transformations

Plasmid Isolation

PCRs: Adding Restriction Sites and Linkers

Restriction Digests

Multi-Ligation: Coming Full Circle

Screening Transformants: Colony PCR with VF2/VR