E.Transformation into E. coli DH5α
The ligation product was transformed into E.coli DH5α strain.
If the vector was pET28a, the strain were grown in LB plate medium containing 10μg/ml kanamycin at 37°C.
If the vector was pBAD30, the strain were grown in LB plate medium containing 100μg/ml ampicillin at 37°C.
If the vector was pSB1C3, the str ain were grown in LB plate medium containing 25μg/ml chloramphenicol at 37°C.