Team:Hong Kong UCCKE/Design
Mechanism of the Uric Acid Concentration-Sensitive Promoter
The promoter is designed to be sensitive to the concentration of uric acid. This promoter controls the expression of the downstream gene. The promoter session consists of a constitutive promoter J23100, a RBS B0034, a strong repressor KRAB-HucR and a double terminator B0015. HucR is itself a repressor. Its repressing ability is enhanced by KRAB. The resulting repressor is a chimeric mammalian urate-dependent transsilencer (mUTS).
Expression of a strong repressor (mUTS)HucO is an operative site for mUTS to bind into. When mUTS is binded to hucO, the expression of downstream gene is restricted according to the concentration of substrates. The presence of uric acid limits the binding of mUTS to hucO. The limitation varies as the concentration of uric acid.
Operator site and Gene of InterestmUTS binds to hucO and GFP is not expressed when uric acid is absent or at very low concentration. Alternatively, the complex detaches from hucO and GFP is expressed according to the concentration of uric acid.
Expression without uric acid (-UA)
Expression with uric acid (+UA)
