Team:UNOTT/Results

RESULTS:

OBJECTIVE: CREATE pgRNA

OBJECTIVE: CREATE REPORTER PLASMID

OBJECTIVE: PROMOTER LIBRARY

OBJECTIVE: RANDOM LIGATIONS

OBJECTIVE: FREEZE DRYING

OBJECTIVE: CRISPRi & guideRNA EFFICIENCY

This picture shows our gRNAs working visually, but the graphs show the results of RFP detection using a microplate reader on cultures containing each of the dCas9 plasmids with one promoter-RFP-terminator brick, with its corresponding gRNA plasmid, or with a non-targeting gRNA plasmid.