RESULTS:
STEP 1: Create guideRNA Plasmid
STEP 2: Create Reporter Plasmid
STEP 3: Create Promoter Library
STEP 4: Random Ligations
STEP 5: Freeze drying & Revivial
STEP 6: CRISPRi & guideRNA efficiency
This picture shows our gRNAs working visually, but the graphs show the results of RFP detection using a microplate reader on cultures containing each of the dCas9 plasmids with one promoter-RFP-terminator brick, with its corresponding gRNA plasmid, or with a non-targeting gRNA plasmid.
What does Marsellus Wallace not look like?
- Black
- Bald
- A Bitch
Malkovich malkovich?
- Malkovich
- Malkovich
- Malkovich
What is your favourite colour?
- Blue
- Yellow
- Not Sure