Team:Virginia/Parts


Basic Parts

  • BBa_K2272000 - a promoter that is maximally induced under microaerobic condition and regulates the expression of VHb, a bacterial hemoglobin that acts as a terminal oxidase.
  • BBa_K2272001 - a promoter that regulates the production of MnSOD (mitochondrial antioxidant manganese superoxide dismutase), which detoxify radical oxygen produced by mitochondrial respiration.
  • BBa_K2272002 - this part encodes Hydroxylamine Oxidoreductase (HAO), which catalyzes the oxidation of hydroxylamine to nitrite
  • BBa_K2272003 - this part encodes two cytochromes that facilitate electron transport during the nitrification process
  • BBa_K2272004 - this sequence from Paracoccus denitrificans codes for a signal peptide that will export the tagged protein to the periplasm of the cell
  • BBa_K2272005 - this sequence from Nitrosomonas europaea codes for a signal peptide that exports the tagged protein to the periplasm of the cell

Composite Parts


  • BBa_K2272012 - a reporter circuit for BBa_K2272001
  • BBa_K2272013 - a reporter circuit for BBa_K2272000
  • BBa_K2272014 - Cytochrome cycA and cycX coding sequence regulated by arabinose-inducible promoter from the distribution kit, pBAD.
  • BBa_K2272015 - Cytochrome cycA and cycX coding sequence regulated by the microaerobic conditioned promoter VHb.
  • BBa_K2272016 - Cytochrome cycA and cycX coding sequence regulated by hypoxic conditioned promoter, MnSOD.
  • BBa_K2272017 - Coding sequence of hydroxylamine oxidoreductase regulated by hypoxic conditioned promoter, MnSOD.
  • BBa_K2272018 - Coding sequence of hydroxylamine oxidoreductase regulated by arabinose-inducible promoter, pBAD.
  • BBa_K2272019 - an composite version of BBa_K1067002 and BBa_K1067004
<groupparts>iGEM17 Virginia</groupparts>