Team:SiCAU-China
SiCAU-China
description
Antibiotic is one of the most popular drug in the world, it can be used in medical treatment, agriculture, food processing, research and so on. But overusing antibiotics may bring antibiotics residue to environment, which will cause potential damage to human and impact ecotope. There are some physical and chemical method to detect antibiotics residue in nowadays , but they often need high cost and complicated process. So this year our team focus on Biosensor, said to be easier way to detect at a lower price than traditional methods, to realize the trace detection.
The positive feedback loop has been widely applied in synthetic biology, such as logic gates, oscillators, bistable circuit. Besides these, the positive feedback loop is also a ultra-sensitive circuit, just like Polymerase Chain Reaction (PCR), even be used in quantitative expression detected. So we engineered a biosensor, which will be established a positive feedback loop using luxI/luxR system in E.coli, to achieve trace detect. Similar to the detect theory of real-time quantitative PCR, there will be a relation between the content of input signal and the time to open the loop. We will use this system to do some antibiotics leftover detection.