Team:UCopenhagen/Parts
Cell measurements
Two colonies from each transformation were picked, and grown in foil-covered 50 ml falcon tubes over night (18 hours).
Preparation OD was measured, and a dilution was calculated to achieve an OD of 0.02. Dilution calculations can be found in the table next to this. Here we used the calculated correction factor from our initial abs/OD calibration. From the absorption measurements taken at 0 hours, we see indications of pipetting errors, as the OD600 (average) ranges from 0.015 to 0.6 (table 1).
| Abs600 | OD600 before | Volume (ml) added to 12 ml media | OD600 after dilution (0 hours) | |
|---|---|---|---|---|
| Negative Control (Colony 1) | 0,461 | 1,435 | 0,167 | 0,06483974 |
| Negative Control (Colony 2) | 0,463 | 1,439 | 0,167 | 0,02584249 |
| Positive Control (Colony 1) | 0,450 | 1,400 | 0,171 | 0,04467949 |
| Positive Control (Colony 2) | 0,474 | 1,475 | 0,163 | 0,01689103 |
| Test Device 1: J23101+I13504 (Colony 1) | 0,462 | 1,437 | 0,167 | 0,03043498 |
| Test Device 1: J23101+I13504 (Colony 2) | 0,472 | 1,469 | 0,163 | 0,01977106 |
| Test Device 2: J23106+I13504 (Colony 1) | 0,477 | 1,483 | 0,162 | 0,03175824 |
| Test Device 2: J23106+I13504 (Colony 2) | 0,454 | 1,410 | 0,170 | 0,018837 |
| Test Device 3: J23117+I13504 (Colony 1) | 0,507 | 1,577 | 0,152 | 0,03261447 |
| Test Device 3: J23117+I13504 (Colony 2) | 0,492 | 1,529 | 0,157 | 0,01471154 |
| Test Device 4: J23101.BCD2.E0040.B0015 (Colony 1) | 0,420 | 1,307 | 0,184 | 0,03759615 |
| Test Device 4: J23101.BCD2.E0040.B0015 (Colony 2) | 0,472 | 1,468 | 0,163 | 0,01860348 |
| Test Device 5: J23106.BCD2.E0040.B0015 (Colony 1) | 0,513 | 1,595 | 0,150 | 0,04234432 |
| Test Device 5: J23106.BCD2.E0040.B0015 (Colony 2) | 0,467 | 1,451 | 0,165 | 0,01494505 |
| Test Device 6: J23117.BCD2.E0040.B0015 (Colony 1) | 0,482 | 1,498 | 0,160 | 0,04452381 |
| Test Device 6: J23117.BCD2.E0040.B0015 (Colony 2) | 0,455 | 1,416 | 0,170 | 0,02008242 |
