Team:IISc-Bangalore/Notebook

  1. Preparation
  2. Week 1
  3. Week 2
  4. Week 3
  5. Week 4
  6. Week 5
  7. Week 6
  8. Week 7
  9. Week 8
  10. Week 9
  11. Week 10

Preparation

19/9/2017

Prepared

  • 500 ml LB
  • 400+200 ml LB Agar
  • 32 petri plates
  • 2x LB plates

Inoculated
  • DH5a in ml SOB (TSS comp cell prep)

20/9/17

1:30 AM

  • Set everything into autoclave

3:00 AM
  • Took everything out of the autoclave
  • Plates dried for 1 hr, agar stored in oven

4:00 AM
  • Plates dried in hood for 1 hr

5:00 AM to 7:00 AM
  • Plates poured
  • Cmp stock conc = 35 mg/ml, working conc = 35 ug/ml

21:30
  • TSS Protocol for comp cell prep

22:10
  • Set secondary culture

21/9/17

Optimizing PCR (set 1)

  • Made reaction mixture of 200 ul per template , for splitting into 8 * 25 ul reactions
  • PCR reaction mixture
    • FP – 4ul
    • RP – 4ul
    • Stock template – 8 ul
    • 2x MM – 100 ul
    • ddH2O – 84 ul
  • Template stock – 100 ng/ul
  • Ran PCR 9:30 PM
  • Ran products on 1% agarose TAE gel (22/9, 12:20 AM)

    Week 1 - DD/MM/YYYYY to DD/MM/YYYY

    Week 2 - DD/MM/YYYYY to DD/MM/YYYY

    Week 3 - DD/MM/YYYYY to DD/MM/YYYY

    Week 4 - DD/MM/YYYYY to DD/MM/YYYY

    Week 5 - DD/MM/YYYYY to DD/MM/YYYY

    Week 6 - DD/MM/YYYYY to DD/MM/YYYY

    Week 7 - DD/MM/YYYYY to DD/MM/YYYY

    Week 8 - DD/MM/YYYYY to DD/MM/YYYY

    Week 9 - DD/MM/YYYYY to DD/MM/YYYY

    Week 10 - DD/MM/YYYYY to DD/MM/YYYY

    Notebook

    Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.

    What should this page have?
    • Chronological notes of what your team is doing.
    • Brief descriptions of daily important events.
    • Pictures of your progress.
    • Mention who participated in what task.
    Inspiration

    You can see what others teams have done to organize their notes: