Team:UCLouvain/Composite Part
in the lab
Parts we created
pCMMCD 105
Plasmid consisting of ComR (BBa_K2341000), the p1655 promoter (containing the ComR box, BBa_K2341001), GusA and an erythromycin resistance.
GusA encodes the beta-glucuronidase gene from E. coli. This is a popular reporter gene that uses glucuronides as substrates, the most common of which are X-gluc (5-bromo-4-chloro-3-indolyl glucuronide) and para-nitrophenyl glucuronide.
Here, it is under the control of the ComRS pathway and uses the ComS peptide from S. thermophilius (LPYFAGGL).
Parts we used
piFinal (BBa_K577882)
iGEM plasmid consisting of araC, a pBAD/AraC promoter (I0500), a RBS(B0034), a RFP(E1010) and two terminators(B0010 and B0012) contained inside a same biobrick (B0015)
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