Team:TecCEM/Demonstrate
Demonstrate
Math modeling with our results
We did a real time PCR, that demonstrates the mRNA expression vs the amounts of siRNA. The theoretical values are demonstrated as T-Xm and the values obtained in the experiment are represented as Xm. The d and θ were adjusted, .005 and 29.1 respectively, the obtained results prove that the transfection efficiency is of 42.9%. The maximum degradation rate observed as d resulted to be even lower than expected, the first value was .001.
The following table shows the values for each point using the equation.
| X | Xm | T-Xm | 0 | 1 | 1 |
|---|---|---|
| 20 | 0.9 | 0.97992 | 40 | 0.6 | 0.632184 | 60 | 0.57 | 0.285714 | 100 | 0.25 | 0.177328 |
