Modeling
We modelled the small single cell bioreactors on drawing programs before building many of them. We made sure that they were cost effective and are reasonable to build. A per unit cost is presented in full detail in the drop down list below where the final total cost is $7.43. This does not include a way to measure the current, which would be about another $30.00 depending on which arduino board (1,2,3) you purchase and how you set it up. This would bring the total cost up to $37.43, which is well within reason for a system that can be reused and will detect harmful contaminants in water. The cost of a drinking water test can range from $50-270. While these tests can detect more contaminants than our system currently can it would only take more genetically engineered Shewanella in order to match the range of tests at a significantly more affordable price.
Single-Chamber Bioreactors
Materials:
- $0.01-$0.04/jar (Bulk discount; MIN = 10,000 units)
- Per unit: $0.04
- Cost: $2.65/8 tubes
- Per unit: $0.33
- Cost: $43.70
- Makes 500 housings
- Per unit: $0.09
- $219.34/2.5 kg
- 1.3 g/housing
- Per unit: $0.01
- 15cm/wire
- Per unit: $0.81
- $52.18 per sheet
- 30 x 30 cm (6.35mm thick)
- 25 x 25 mm/unit
- 144 electrodes per sheet
- Per unit: $0.36
- $0.05 ea.
- x 2 per unit
- Per unit: $0.10
- Per unit: $0.04
- trade size #12 (2 1/2", tapered to 2 1/8")
- Per unit: $2.31
- trade size C (3/8" tapered to 7/32")
- Per unit: $0.71
- Per stir bar: $3.96
- Bulk unit via MSU chemistry store
- Per unit: $1.60
- $48.20
- Makes 75 eletrodes
- Per unit: $0.64
- Per 500ml: $0.01
- Cost: $39.26
- Package has 100 needles
- Per unit: $0.39
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200 mL mason jar
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Glass reference electrode housing
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Magnesia sticks (needed to complete housing)
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Potassium Chloride
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Titanium wire
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Carbon felt
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Sterile 18G needles
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3mL syringe
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Rubber stopper
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Rubber stopper
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Octogonal magnetic stir bars with pivot ring
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Leit-C for microscopy (carbon cement for electrodes)
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Xylene
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18G Hypodermic sampling needle (4")
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References
(1) Aristizabal, D. H.; Giraldo, D. A.; Sanchez, S.; Taborda, G.; Baeza, A. J. Phys. Conf. Ser. 2017, 365, 11001.
(2) Jannelli, N.; Anna Nastro, R.; Cigolotti, V.; Minutillo, M.; Falcucci, G. Appl. Energy 2017, 192, 543–550.
(3) Yang, Y.; Ren, H.; Ben-Tzvi, P.; Yang, X.; He, Z. Int. J. Hydrogen Energy 2017, 42 (31), 20260–20268.
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