Team:SDSZ-China/Parts

• Parts

Name	Type	Description	Designer	Length
BBa_K2239001	Generator	ChBD-7alphaHSDH	Ruohong Wang	1089
BBa_K2239002	Generator	ChBD-7betaHSDH	Ruohong Wang	1179
BBa_K2239003	Generator	ChBD-LDH	Ruohong Wang	1323
BBa_K2239004	Generator	ChBD-GDH	Ruohong Wang	1107
BBa_K2239005	Generator	ChBD-GFP	Ruohong Wang	1047
BBa_K2239006	Generator	CBD-7alphaHSDH	Ruohong Wang	1080
BBa_K2239007	Generator	CBD-7betaHSDH	Ruohong Wang	1170
BBa_K2239008	Generator	CBD-LDH	Ruohong Wang	1314
BBa_K2239009	Generator	CBD-GDH	Ruohong Wang	1098
BBa_K2239010	Generator	CBD-GFP	Ruohong Wang	1038
BBa_K2239011	Coding	7alpha-HSDH	Ruohong Wang	780
BBa_K2239012	Coding	7beta-HSDH	Ruohong Wang	870
BBa_K2239013	Coding	GFP	Ruohong Wang	738
BBa_K2239014	Coding	GDH (glucose dehydrogenas)	Ruohong Wang	798
BBa_K2239015	Coding	LDH ( lactate dehydrogenase)	Ruohong Wang	1014

7alpha-HSDH

7alpha-HSDH (7alpha-hydroxysteroid dehydrogenase) catalyzes the oxidation of hydroxysteroids at C-7 position and back, as it converts NAD+ to NADH and back. It catalyzes the oxidation of CDCA into intermediate product 7-oxo-LAC (7-ketolithocholic acid), where the hydroxyl at C-7 position becomes carbonyl.[1]

Sequence and Features

Subparts|Ruler|SS|DSLength: 780 bpGet part sequence.View plasmid

. .1 11 21 31 41 51 61 71 81 91

1 100 200 300 400 500 600 700 800

7alpha-HSDH

his-tag

catcatcatc atcatcactt taattctgac aacctgagac tcgacggaaa atgcgccatc atcacaggtg cgggtgcagg tattggtaaa gaaatcgcca

1

gtagtagtag tagtagtgaa attaagactg ttggactctg agctgccttt tacgcggtag tagtgtccac gcccacgtcc ataaccattt ctttagcggt

7alpha-HSDH

his-tag

ttacattcgc gacagctggc gcatctgtgg tggtcagtga tattaacgcc gacgcagcta accatgttgt agacgaaatt caacaactgg gtggtcaggc

101

aatgtaagcg ctgtcgaccg cgtagacacc accagtcact ataattgcgg ctgcgtcgat tggtacaaca tctgctttaa gttgttgacc caccagtccg

7alpha-HSDH

atttgcctgc cgttgtgata ttacttccga acaggaactc tctgcactgg cagactttgc cgtcagtaag ctgggtaaag ttgatatcct ggttaacaac

201

taaacggacg gcaacactat aatgaaggct tgtccttgag agacgtgacc gtctgaaacg gcagtcattc gacccatttc aactatagga ccaattgttg

7alpha-HSDH

gccggtggcg gtggtcctaa accgtttgat atgccaatgg cagattttcg ccgtgcttat gaactgaatg tgttttcttt tttccatctg tcacaacttg

301

cggccaccgc caccaggatt tggcaaacta tacggttacc gtctaaaagc ggcacgaata cttgacttac acaaaagaaa aaaggtagac agtgttgaac

7alpha-HSDH

ttgcgccaga aatggaaaaa aatggcggtg gcgttattct gaccatcact tctatggcgg cagaaaataa aaatataaac atgacttcct atgcatcatc

401

aacgcggtct ttaccttttt ttaccgccac cgcaataaga ctggtagtga agataccgcc gtcttttatt tttatatttg tactgaagga tacgtagtag

7alpha-HSDH

taaagctgcg gccagtcatc tggtcagaaa tatggcattt gacctgggtg aaaaaaatat tcgggtgaat ggcattgcgc cgggggcaat attaaccgat

501

atttcgacgc cggtcagtag accagtcttt ataccgtaaa ctggacccac tttttttata agcccactta ccgtaacgcg gcccccgtta taattggcta

7alpha-HSDH

gccctgaaat ccgttattac accagaaatt gaacaaaaaa tgttacagca cacgccgatc atacgtctgg gccaaccgca agatattgct aacgcggcgc

601

cgggacttta ggcaataatg tggtctttaa cttgtttttt acaatgtcgt gtgcggctag tatgcagacc cggttggcgt tctataacga ttgcgccgcg

7alpha-HSDH

tgttcctttg ctcgcctgct gccagctggg taagcggaca aattctcacc gtctccggtg gtggggtaca ggagctcaat

701

acaaggaaac gagcggacga cggtcgaccc attcgcctgt ttaagagtgg cagaggccac caccccatgt cctcgagtta

7alpha-HSDH

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]