Team:UCopenhagen/Improve
Improved biobrick
We have improved the function of a previous Biobrick: Original biobrick; http://parts.igem.org/Part:BBa_K864100. Our improved biobrick: http://parts.igem.org/Part:BBa_K2455002. The Biobricks are well characterised on their respective pages, so we will not list all the same information on this page.
BBa_K2455002 is an improved version of the Super Yellow Fluorescent Protein 2 (BBa_K864100) carrying an N-terminal nona-arginine tag, and a C-terminal hexa-histidine tag. The R9-tag is a Cell-Penetrating Peptide giving it's associated protein the ability to pass through plasma membranes.
We made the improvement by inserting BBa_K864100 into the USER casette of our third biobrick, the Cell-Penetrating USER Cassette http://parts.igem.org/Part:BBa_K2455003.
This biobrick contains an AsiSI/Nb.BSMI USER Cassette with a N-terminal nona-arginine (R9) Cell-penetrating peptide.
The nona-arginine (R9) gives any cassette inserted proteins the ability to pass through plasma membranes.
In addition, the USER Cassette carries a N-terminal hexa-histidine tag which allows for easy purification.
For our study we demonstrated that proteins inserted into the cassette can be purified using the histidine-tag,
and subsequently used to stain E. coli and Pseudomonas putida cells, presumably through internalization caused by the R9 peptide.
See the results page on this wiki, or go to the parts-pages for more information.
