If you wish to store bacteria long term, you will need to create a Glycerol Stock after
inoculating an overnight liquid culture
- Once bacterial growth has been achieved, 500μL of the overnight liquid
culture needs to be added to 500μL of 50% glycerol in a 2mL tube where it
should be gently mixed
- The glycerol stock should then be frozen at -80 o C
- Successive freeze and thaw cycles will reduce the stocks shelf life