Obviously, we are aiming to synthesize a protein that can be used for human treatments. For this to be achieved, it must be safe to use in humans. Therefore, we had to ensure that we had only conserved mutations, or mutations that had been previously done and shown to have no adverse reactions during clinical trials. Luckily, other than general allergic reactions, we came across no mutations that had been linked to any adverse reactions in Proinsulin. For Winsulin, we do have to trial whether the 12AA C Peptide is immunogenic or whether it works. However, we ensured that the A and B chains of insulin remained homologous to the native human insulin sequence.
Winsulin : Single Chain Insulin with a 12 AA linker peptide