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Revision as of 16:32, 17 September 2017
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Bioreactor simulation
The yield of $154.9 \ \frac{\text{mg}}{\text{L}}$ Nootkatol was lower than the published results of Wriessnegger 2014. Our first guess was that the assumption of all enzymes being equally concentrated was probably false. We therefore varied the enzyme concentrations and found that overexpression of valencene synthase increased the yield dramatically by converting way more FPP than before, while overexpressing the other enzymes had little to no effect at all:
What can be seen in the plot above is that the system is mostly influenced by concentration changes of valencene synthase. Since the Nootkatone production did not seem to increase further after increasing the valencene synthase concentration by 20-fold, we stuck to that number and simulated our model under the changed conditions.
