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NrtA Functional Test | NrtA Functional Test | ||
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<p> To test whether NrtA protein could capture nitrate and nitrite, we used French Pressure Press to isolate NrtA protein, and used Cayman Nitrate/Nitrite Colorimetric Assay Kit to measure the nitrate and nitrite concentration in the medium with NrtA protein. (You can see the detail in our Notebook page: <a href="https://2017.igem.org/Team:NYMU-Taipei/Notebook">click</a>.)</p> | <p> To test whether NrtA protein could capture nitrate and nitrite, we used French Pressure Press to isolate NrtA protein, and used Cayman Nitrate/Nitrite Colorimetric Assay Kit to measure the nitrate and nitrite concentration in the medium with NrtA protein. (You can see the detail in our Notebook page: <a href="https://2017.igem.org/Team:NYMU-Taipei/Notebook">click</a>.)</p> | ||
<p> Here are our results.</p> | <p> Here are our results.</p> | ||
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<img src="https://static.igem.org/mediawiki/parts/8/8c/T--NYMU-Taipei--NS_NrtA_func_1.png" style="width:95%"> | <img src="https://static.igem.org/mediawiki/parts/8/8c/T--NYMU-Taipei--NS_NrtA_func_1.png" style="width:95%"> | ||
<p style="font-size:16px;">Figure1. Nitrate concentration of cell lysate. | <p style="font-size:16px;">Figure1. Nitrate concentration of cell lysate. | ||
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<p> Then we wanted to know whether the engineered <i>E.coli</i> could decrease nitrate and nitrite in the environment. We used Cayman Nitrate/Nitrite Colorimetric Assay Kit to measure the nitrate and nitrite concentration of the supernatant of the engineered <i>E.coli</i> liquid culture.</p> | <p> Then we wanted to know whether the engineered <i>E.coli</i> could decrease nitrate and nitrite in the environment. We used Cayman Nitrate/Nitrite Colorimetric Assay Kit to measure the nitrate and nitrite concentration of the supernatant of the engineered <i>E.coli</i> liquid culture.</p> | ||
<p>Here are our results.</p> | <p>Here are our results.</p> | ||
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<img src="https://static.igem.org/mediawiki/2017/0/0d/NYMU_2017_awards_basicpart_2.jpg" style="width:95%"> | <img src="https://static.igem.org/mediawiki/2017/0/0d/NYMU_2017_awards_basicpart_2.jpg" style="width:95%"> | ||
<p style="font-size:16px;">Figure 2. Nitrate concentration of cell. | <p style="font-size:16px;">Figure 2. Nitrate concentration of cell. | ||
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Revision as of 17:55, 28 October 2017