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<h4></h4> | <h4></h4> | ||
<h4>NrtA Protein</h4> | <h4>NrtA Protein</h4> | ||
− | <p> To test whether NrtA protein could capture nitrate and nitrite, we used French Pressure Press to isolate NrtA protein, and used Cayman Nitrate/Nitrite Colorimetric Assay Kit to measure the nitrate and nitrite concentration in the medium with NrtA protein. (You can see the detail in our Notebook page: <a href="https://2017.igem.org/Team:NYMU-Taipei/Notebook">click</a>.)</p> | + | <p> To test whether NrtA protein could capture nitrate and nitrite, we used <font class='mark_backbone'>French Pressure Press</font> to isolate NrtA protein, and used <font class='mark_backbone'>Cayman Nitrate/Nitrite Colorimetric Assay Kit</font> to measure the nitrate and nitrite concentration in the medium with NrtA protein. (You can see the detail in our Notebook page: <a href="https://2017.igem.org/Team:NYMU-Taipei/Notebook">click</a>.)</p> |
<p> Here are our results.</p> | <p> Here are our results.</p> | ||
<div style="width:60%;margin:auto"> | <div style="width:60%;margin:auto"> | ||
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</div> | </div> | ||
<br> | <br> | ||
− | <p> The nitrate and nitrite concentration of competent cell and NrtA was significant different. The results indicated that NrtA protein can capture nitrite and nitrate! This is a milestone of our project!</p> | + | <p> The nitrate and nitrite concentration of competent cell and NrtA was significant different. The results indicated that <font class='mark_backbone'>NrtA protein can capture nitrite and nitrate</font>! This is a milestone of our project!</p> |
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<br> | <br> | ||
− | <p> The nitrate and nitrite concentration of NrtA and competent cell had slight but not significant difference. The result implied that NrtA protein could capture nitrate and nitrite while the engineered <i>E.coli</i> with NrtA gene couldn’t. The engineered <i>E.coli</i> with NrtA gene could express NrtA protein, while NrtA protein might be inside the cell, so nitrate and nitrite concentration outside the cell didn’t change a lot. To make NrtA protein become a secreted protein, we are trying to construct signal peptide sequence, so that NrtA protein can be released to the medium.</p> | + | <p> The nitrate and nitrite concentration of NrtA and competent cell had slight but not significant difference. The result implied that NrtA protein could capture nitrate and nitrite while the engineered <i>E.coli</i> with NrtA gene couldn’t. The engineered <i>E.coli</i> with NrtA gene could express NrtA protein, while NrtA protein might be inside the cell, so nitrate and nitrite concentration outside the cell didn’t change a lot. To make NrtA protein become a secreted protein, we are trying to construct <font class='mark_backbone'>signal peptide sequence</font>, so that NrtA protein can be released to the medium.</p> |
Revision as of 18:04, 28 October 2017