Difference between revisions of "Team:GZHS-United/Notebook"

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1. Acquire Cry4Ba gene form genomic DNA of Bt by PCR.<br>
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2. Purification the PCR product of mtx1 .<br>
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3. Construction of cloning vector for direct ligation with mtx1’s purified product . <br>
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4. Transform the plasmid into E.coli.
 
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1. The Cry4Ba gene was successfully obtained.<br>
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<img src="https://static.igem.org/mediawiki/2017/7/75/T--GZHS-United--notebook3.png" class="img100" ><br>
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2. A series of Experiment were performed successfully.
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Revision as of 18:12, 28 October 2017

Notebook

Date objection result Analysis Solution
7-10 1. Extract the genomic DNA of Bt and Bs. 1. Only the genomic DNA of Bs were obtained. The Bt haven’t been actived. Culture Bt in LB to active it.
7-11 1. Extract the genomic DNA of Bt.
2. Acquire mtx gene form genomic DNA of Bs by PCR. 		1. DNA electrophoresis showed that the strip appeared at 150bp.
2. A peal DNA strip in agarose gel electrophoresis were observed. 		1. The fragment we extracted were RNA. 1. Use a more gentle method to broke the Gram-negative bacterium cell wall.
2. Optimize the PCR condition and try to use the diluted production as template.
7-12 1. Extract the genomic DNA of Bt.
2. Amplification of mtx1 gene. 		1. The genomic DNA of Bt were successfully obtained.

2. The mtx1 gene was successfully obtained. 		Boiling water-bath is a good method to broke the bacterium cell wall. /
7-13 1. Acquire Cry4Ba gene form genomic DNA of Bt by PCR.
2. Purification the PCR product of mtx1 .
3. Construction of cloning vector for direct ligation with mtx1’s purified product .
4. Transform the plasmid into E.coli. 		1. The Cry4Ba gene was successfully obtained.

2. A series of Experiment were performed successfully. 		qqq qqq
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