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</a> | </a> | ||
<p style="padding-top:10px;"></p> | <p style="padding-top:10px;"></p> | ||
− | <h4>NrtA | + | <h4>NrtA Expression</h4> |
<p> We successfully transformed NrtA gene from cyanobacteria <i>Synechocystis</i> sp. PCC 6803 to <i>E.coli</i>.</p> | <p> We successfully transformed NrtA gene from cyanobacteria <i>Synechocystis</i> sp. PCC 6803 to <i>E.coli</i>.</p> | ||
<center><img src="https://static.igem.org/mediawiki/2017/d/da/T--NYMU-Taipei--NS_NrtA_RE.png" style="width:60%;"></center> | <center><img src="https://static.igem.org/mediawiki/2017/d/da/T--NYMU-Taipei--NS_NrtA_RE.png" style="width:60%;"></center> | ||
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<h4></h4> | <h4></h4> | ||
− | <h4> | + | <h4>Endolysin Construct</h4> |
<p> We successfully constructed J23106-B0034-Endolysin-B0010-B0012. The endolysin in this part is from iGEM released part, BBa_K112806, which is endolysin from enterobacteria phage T4. Besides endolysin, in this composite part, we choose BBa_J23106 as a constitutive promoter, BBa_B0034 as ribosome binding site, BBa_B0010 and BBa_B0012 as double terminator, all of which are widely used parts in iGEM.</p> | <p> We successfully constructed J23106-B0034-Endolysin-B0010-B0012. The endolysin in this part is from iGEM released part, BBa_K112806, which is endolysin from enterobacteria phage T4. Besides endolysin, in this composite part, we choose BBa_J23106 as a constitutive promoter, BBa_B0034 as ribosome binding site, BBa_B0010 and BBa_B0012 as double terminator, all of which are widely used parts in iGEM.</p> | ||
<div style="width:55%;margin:auto;"> | <div style="width:55%;margin:auto;"> | ||
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<h4></h4> | <h4></h4> | ||
− | <h4> | + | <h4>Holin Construct</h4> |
<p> We successfully constructed R0010-B0034-Holin-B0010-B0012. To control the precise suicide timing, we choose a lactose-induced promoter, BBa_R0010, to regulate this suicide mechanism. Besides holin and inducible promoter, in this composite part, we also choose BBa_B0034 as ribosome binding site, and BBa_B0010 and BBa_B0012 as double terminator.</p> | <p> We successfully constructed R0010-B0034-Holin-B0010-B0012. To control the precise suicide timing, we choose a lactose-induced promoter, BBa_R0010, to regulate this suicide mechanism. Besides holin and inducible promoter, in this composite part, we also choose BBa_B0034 as ribosome binding site, and BBa_B0010 and BBa_B0012 as double terminator.</p> | ||
<div style="width:55%;margin:auto;"> | <div style="width:55%;margin:auto;"> | ||
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<h4></h4> | <h4></h4> | ||
− | <h4> | + | <h4>Endolysin-Holin Construct</h4> |
<p> We successfully constructed R0010-B0034-Holin-B0010-B0012-J23106-B0034-Endolysin-B0010-B0012. We combined holin and endolysin for suicide mechanism. In this composite part, holin functions as an important regulation role.</p> | <p> We successfully constructed R0010-B0034-Holin-B0010-B0012-J23106-B0034-Endolysin-B0010-B0012. We combined holin and endolysin for suicide mechanism. In this composite part, holin functions as an important regulation role.</p> | ||
<div style="width:55%;margin:auto;"> | <div style="width:55%;margin:auto;"> | ||
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<h4></h4> | <h4></h4> | ||
− | <h4> | + | <h4>Endolysin-Holin-NrtA Construct</h4> |
<p> We successfully constructed R0010-B0034-Holin-B0010-B0012-J23106-B0034-Endolysin-B0010-B0012-J23118-B0034-NrtA-B0015. This part combined holin, endolysin, and NrtA and had nitrate-capturing function and suicide function.</p> | <p> We successfully constructed R0010-B0034-Holin-B0010-B0012-J23106-B0034-Endolysin-B0010-B0012-J23118-B0034-NrtA-B0015. This part combined holin, endolysin, and NrtA and had nitrate-capturing function and suicide function.</p> | ||
<div style="width:55%;margin:auto;"> | <div style="width:55%;margin:auto;"> |
Revision as of 20:05, 28 October 2017